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Aspects of a steel-framed building’s life cycle that influence its environmental sustainability
No full textThe assessment of the environmental impact caused throughout the life cycle of a structure is a major step towards optimizing its sustainability. Based on methodologies such as life cycle assessment, these calculations can be used to determine the life cycle stages -as well as the very processes or materials-that are responsible for the largest environmental impacts. It is then in the hands of those carrying out the construction works to incorporate this knowledge into standard project delivery with the aim to minimize environmental impacts and ultimately increase the sustainability of the construction sector. This research aims to materialize this prospect by investigating the environmental impact caused by the life cycle of a steel-framed building in order to determine the aspects that significantly influence its sustainability. An existing building is selected as the basis of the calculations and the influence of several factors is assessed through the results that are obtained. The conclusions drawn highlight the issues that affect the environmental sustainability of steel construction, while also furthering knowledge concerning the determination of factors and criteria that can be used as the basis for recommendations that can be applied to similar projects is produced
A comparison study in the processing procedure of data from untargeted metabolomic approaches by UPLC-ESI(-)-HRMS
Full text linkMDR-involved human glutathione transferases (hGSTs) are targets for inhibition by 2,2'-dihydroxybenzophenones and N-carbonyl analogues
Full text linkOver expression of human GSTA1-1 in tumour cells is part of MDR mechanisms. Substituted 2-hydroxybenzophenones are ubiquitous in naturally occurring and synthetic compounds, exhibiting important biological activities. 2,2’-Dihydroxybenzophenones and N-carbonyl analogues, structurally, are ringopened forms of xanthone analogues which we reported recently as hGSTA1-1 inhibitors. The present study combined GST inhibition screening, in silico molecular docking and enzyme inhibition kinetics, revealing four analogues with strong inhibitory potency (IC50 = 0.18-1.8 μM) and modest cytotoxic activity for Caco2 cell line (LC50 = 35 to > 400 μM), thus being useful as lead structures for the design of new inhibitors against hGSTs
Επίδραση της διατροφικής χορήγησης εσπεριδίνης και ναρινγίνης στο επίπεδο του οξειδωτικού stress σε δείγματα πλάσματος αίματος αμνών
No full textEffects of flavonoids dietary supplementation on strained yoghurt antioxidant capacity
Full text linkCombinatorial design, selection and synthesis of peptide inhibitors against human glutathione transferase p1-1
Full text linkCertain glutathione S-transferase (GST) isoenzymes detoxify the cell from xenobiotics, thus becoming inhibition targets when overexpressed in various tumours leading to MDR. We developed a combinatorial strategy aiming at designing peptide inhibitors against the hGSTP1-1 isoenzyme involved in MDR. We employed a combinatorial peptide library featuring engineered E. coli cells harboring a plasmid able to express a fusion protein containing random 12 peptides which were inserted into the active loop of thioredoxin, which itself was inserted into the dispensable region of the flagellin gene. After five selection rounds, clones were screened for hGSTP1-1 binding and those with the strongest signal were selected and sequenced. Sequence alignments showed a core binding sequence which, along with selected peptide fragments, were synthesized using the solid phase methodology and Fmoc/tBu chemistry on 2-chlorotrityl chloride solid support. The four peptides were studied for their inhibition potency against hGSTP1-1 allozymes A, B and C
Μελέτη της ικανότητας του βακτηρίου Salmonella Typhimurium να σχηματίζει βιο- υμένιο σε επιφάνεια πολυστυρενίου παρουσία μορίων-σημάτων διακυτταρικής επικοινωνίας
Full text linkΑυτή η μελέτη είχε σκοπό την αξιολόγηση της επίδρασης των AHLs μικροβιακής προέλευσης στην ικανότητα σχηματισμού βιο-υμενίου από το βακτήριο S. enterica ορότυπος Typhimurium (SeT) σε αβιοτική επιφάνεια. Το SeT αφέθηκε να σχηματίσει βιο-υμένιο σε μικροπλακίδιο πολυστυρενίου 96-βοθρίων στους 20˚C για συνολικό χρονικό διάστημα 120 ωρών, με τη χρήση δύο μέσων ανάπτυξης (TSB και αραιωμένο TSB, αTSB). Αμφότερα τα μέσα ανάπτυξης εμπλουτίστηκαν με AHLs, τα οποία λήφθησαν μετά την εκχύλιση με οξικό αιθυλεστέρα καλλιέργειας Hafnia alvei, την εξάτμιση του οργανικού διαλύτη και την επαναιώρηση στα προαναφερθέντα μέσα ανάπτυξης (50% ν/ν AHLs).
This study aimed to evaluate the effect of microbial AHL molecules on the biofilm-formation ability of S. enterica serovar Typhimurium (SeT) on an abiotic substratum. SeT was left to form biofilms on 96-well polystyrene microplates at 20 ˚C for a total period of 120h under two growth conditions (TSB and diluted TSB, dTSB). Both growth media were supplemented by evaporated ethyl acetate AHL extracts of Hafnia alvei culture re-diluted in the aforementioned media (50% v/v AHLs)
Τεχνική αναφορά μεθοδολογίας επεξεργασίας δειγμάτων σταθερών ισοτόπων, ολικού οργανικού άνθρακα και πετρελαϊκού δυναμικού
Full text linkMonitoring the growth of Salmonella enterica serovar typhimurium in silico and in situ with a view in gene expression
Full text linkIn the present study, the ability of S. Typhimurium to develop a biofilm community on rocket tissue was investigated at 20°C. The differences on expression of genes associated with several functional roles during growth of S. Typhimurium on rocket extract and rocket tissue regarding a laboratory growth medium (Luria – Bertani broth, LB) was also monitored. The findings of the present study could show that Salmonella reacts as exposed to different types of stress when inoculated to a heat sterile plant extract and plant tissue. However, further studies are needed to better determine the survival and / or growth of these as “real” biofilm cells on plant tissues