Kenyon College
Kenyon College: Digital Kenyon - Research, Scholarship, and Creative ExchangeNot a member yet
135065 research outputs found
Sort by
Yeni Valide Mosque
No full texthttps://digital.kenyon.edu/arthistorystudycollection/2761/thumbnail.jp
Funerary complex of Sultan Qaytbay
No full texthttps://digital.kenyon.edu/arthistorystudycollection/2781/thumbnail.jp
Mosque-Madrasa of Sultan Hasan, Interior
No full texthttps://digital.kenyon.edu/arthistorystudycollection/2794/thumbnail.jp
Great Mosque of Diyarbakır
No full texthttps://digital.kenyon.edu/arthistorystudycollection/2803/thumbnail.jp
Mosque of Ibn Tulun
No full texthttps://digital.kenyon.edu/arthistorystudycollection/2823/thumbnail.jp
Temporal Frames and Linguistic Identity: Exploring Connections Between Lived Experience with Language and Grammatical Choices
No full textExposure to a second language is a common experience in the American education system, but the impacts of second language learning on one’s linguistic identity are often overlooked in studying the process of language acquisition. This study compared a focus group of six individuals who had studied French in the past with two case studies consisting of two current graduate students in French and one native speaker. All participants were from the central Ohio region. The participants were asked to tell a fictional narrative based on the book Frog, Where Are You? by Mercer Mayer, once in English and once in French. Afterwards, the participants were asked to describe their fluency subjectively and objectively by filling out a language history. In order to examine expression of linguistic identity, the narratives told by participants were analyzed for use of verb tense and the establishment of temporal frames throughout the story. Taken with the information provided by participants about their language learning, it was found that students employed a larger variety of verb tenses in their French narratives in order to establish background and temporal boundaries, while the adults tended to tell narratives in the present tense with less complexity. This is reflective of the difference between a population currently studying French, who would have more access to increased complexity of language, and a population receiving little to no formal instruction in the language during this time. Linguistic identity was expressed, however, in a contradictory manner, with students expressing less attachment to the French language and culture and adults experiencing a strong nostalgia about their time as a student of French
Generating CRISPR-Cas9 plasmids for targeted gene knockouts in Physcomitrium patens via Golden Gate cloning
No full textThe moss P. patens utilizes seasonal cues, such as daylength and temperature, to regulate the timing of its sexual reproduction. We are using a variety of forward and reverse genetic approaches to identify candidate genes that are involved in this regulatory pathway. To determine if a gene is necessary for seasonal reproduction in P. patens, we use CRISPRCas9 technology to induce deleterious mutations. In the past, we have created knockouts of candidate genes with a CRISPR-Cas9 system by transforming multiple bacterial plasmids into protoplasts, including at least three separate plasmids for expressing the endonuclease Cas9, a single guide RNA (sgRNA), and an antibiotic resistance gene. We’ve simplified this process by utilizing a destination vector containing Cas9 and selectable markers to then load an sgRNA onto the plasmid via Golden Gate Cloning (Mallett et al. 2019, Prigge and Estelle). This method possesses numerous advantages, including cheaper costs and greater simplicity for protoplast transformation protocols