Kenyon College
Kenyon College: Digital Kenyon - Research, Scholarship, and Creative ExchangeNot a member yet
135065 research outputs found
Sort by
Human Proximity Has No Significant Influence on Pika (Ochotona princeps) Gut Microbiome Diversity In Glacier National Park
No full text
Regarding Chinese Conceptual Art (Essay)
No full textThis is an essay, titled On Conceptual Art in China, written by the Paris-based art critic about the origin and state of Chinese Conceptual Art. The corresponding document is an abstract of the essay written by Zhou Yan. (Nicole Wang \u2726).https://digital.kenyon.edu/zhoudocs/1508/thumbnail.jp
Blue Mosque, Interior
No full texthttps://digital.kenyon.edu/arthistorystudycollection/2738/thumbnail.jp
Miliaresion of Romanos III
No full textThe obverse of this coin depicts a full-length, standing portrait of the Virgin turned slightly to the right. She is nimbate (haloed) and wearing a tunic and maphorion (veil). The Virgin also stands on a dais. In her left arm she is holding the infant Christ, who raises his right hand in the benediction. The infant holds a scroll in his left hand. The Virgin’s right hand rests on her breast. The figures are surrounded by an inscription of Greek letters and a decorative border.
The reverse of this coin depicts a full-length portrait of the emperor Romanos III standing on a round cushion, bearded, wearing a crown with a cross and pendilia (gemmed pendants). He also wears a modified loros (long decorated scarf). In his right hand, Romanos holds a long patriarchal cross, and in his left hand, a globe surmounted by a cross. The same decorative border as on the obverse encircles him.
Sonia Suben 2025https://digital.kenyon.edu/arthistorystudycollection/2711/thumbnail.jp
Characterization of functionally important residues in the transport mechanism of Aedes aegypti cation chloride cotransporter 2 (aeCCC2)
No full textAedes aegypti cation chloride cotransporter 2 (aeCCC2) belongs to a family of Na+-dependent cation chloride cotransporters which includes Na+-K+-2Cl- (NKCC) cotransporters (Duong et al. 2022). Despite high sequence similarity to the electroneutral NKCCs, aeCCC2 transports ions electrogenically, suggesting a transport mechanism that has differentiated from the NKCCs (Kalsi et al. 2019). In this thesis, we use site-directed mutagenesis and a Xenopus laevis expression system to explore residue-specific contributions to the transport properties of aeCCC2 in order to further investigate its transport mechanism. We found that aeCCC2-mediated transport saturated at low extracellular ion concentrations. Furthermore, single mutations at ion-binding residues L155 or W158 decreased ion affinity three to four-fold, while constructs carrying mutations at both L155 and W158 had a ten-fold decrease in ion affinity. aeCCC2 mutants had decreased Li+ uptake rates and increased Rb+ uptake rates in comparison to aeCCC2 WT. In fact, the Li+:Rb+ uptake rate ratio was roughly two to four-fold lower than aeCCC2 WT in aeCCC2 mutants. To explore the effects of hypotonic activation on aeCCC2-mediated transport, we measured the ion affinity of aeCCC2 in response to hypotonic stimulation, as well as the Li+ transport rate of aeCCC2 constructs carrying mutations at the predicted SPS/Ste20 proline-alanine-rich kinase (SPAK)-binding site. Hypotonic activation does not alter the ion affinity of aeCCC2, and aeCCC2-mediated Li+ uptake is abolished in kinase-binding mutants. Altogether, these results suggest a carrier-like mechanism of aeCCC2-mediated transport that is dependent on basal activation by the with no lysine (WNK)/SPAK kinase complex, where L155 and W158 both contribute to ion binding and may contribute to ion selectivity