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Manufacturing and Evaluation of Mechanical Properties for Rice Husk ParticleBoard Using IoT
Get PDFIn recent times, different types of particleboards are being preferred in the construction of houses, partitions, furnitureetc. The production of such materials can be manufactured using rice husk, which has been obtained as waste produced inrice millers. Adhesive such as formaldehyde, when exposed to fire, causes toxic flames which are fatal in nature. The basiccondition for production of particleboards is to check the temperature and humidity content in the rice husk which has beendone by using DHT 11 sensors i.e., application of Internet of Thing (IoT) erected method. This identification helps infinding the suitable temperature through which bio-based adhesives have been prepared. In present study, two differenttypes of bio-adhesives namely tamarind with formalin and tamarind with boric acid has been used in manufacturing process.The application of IoT erected method follows a complex preparation method but will partially fulfil the job and reduceshuman involvement. Finally, when the proper temperature and moisture level has been measured, the preparation becomeseasy. After manufacturing the particleboard, the strength has been tested by a three-point bend test and have been comparedwith commercially available boards with formaldehyde base adhesive
Differential gene expression analysis in germinating and dormant teliospores of Tilletia indica using RNA seq approach
Get PDFKarnal bunt of wheat is an important quarantine disease that interrupts India’s wheat trade in the international market. The whole transcriptome of germinating and dormant teliospores of Tilletia indica was performed using the RNA Seq approach to identify germination-related genes. Approximately 63 million reads were generated using the RNA sequencing by the Illumina NextSeq500 platform. The high-quality reads were deposited in NCBI SRA database (accession: PRJNA522347). The unigenes from the pooled teliospores were 16,575 having unigenes length of 28,998,753 bases. The high-quality reads of germinating teliospores mapped on to 21,505 predicted CDSs. 9,680 CDSs were common between dormant and germinating teliospores of T. indica. 11,825 CDSs were found to be in germinating teliospores while only 91 were unique in dormant spores of pathogen. The pathway analysis showed the highest number of pathways was found in germinating spores than dormant spores. The highest numbers of CDSs were found to be associated with translation (431 in number), transport and catabolism (340), signal transduction (326), and carbohydrate metabolism (283). The differential expression analysis (DESeq) of germinating and dormant teliospores showed that 686 CDS were up-regulated and 114 CDS were down-regulated in the germinating teliospores. Significant germination-related genes in the spores were validated using qPCR analysis. Ten genes viz. Ti3931, Ti6828, Ti7098, Ti7462, Ti7522, Ti 9289, Ti 8670, Ti 7959, Ti 7809,and Ti10095 were highly up-regulated in germinated teliospores which may have role in germination of spores.Further, these differentially expressed genes provide insights into the molecular events. This first study of transcriptome will be helpful to devise better management strategies to manage Karnal bunt disease
Re-engineering of bicistronic plasmid pGPD/IFN to construct fusion gene co-expressing Glyceraldehyde 3-phosphate dehydrogenase gene (GAPDH) of Edwardsiella tarda and Interferon-gamma (IFN-γ) gene of Labeo rohita (Hamilton) and its in vitro functional analysis
Get PDFEdwardsiella septicemia disease in the cultured Indian major carps is caused by the fish pathogen Edwardsiella tarda and it is preventable by DNA vaccination. Here, we tried to develop a bicistronic DNA vaccine pGPD/IFN expressing the Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene of Edwardsiella tarda and Interferon-gamma (IFN-γ) gene of Labeo rohita. The vaccine showed high protective efficiency in our previous studies; however as a limitation of bicistronic construct the expression of gene cloned in second frame (B) is poor. To overcome this limitation we re-engineered the construct and designed a fusion gene co-expressing the GAPDH and IFN-γ genes as one frame with an aim to get the optimum expression of both the genes. For this purpose, a fusion insert comprising GAPDH and IFN-γ coding sequences was cloned in to pcDNA3.1(+) plasmid vector. The fusion genes' in vitro expression was confirmed in the striped snakehead fish cell line (SSN-1). Successful expression of the re-engineered fusion gene DNA vaccine in the cell line was achieved at 48h post-transfection, which was confirmed by amplifying the expression transcripts of GAPDH and IFN-γ genes. Thus, the study concludes that the re-engineered fusion vaccine pcGPD/IFN (pcDNA3.1(+) plasmid having fusion GPD/IFN) is functional and can be effectively utilized to vaccinate rohu (Labeo rohita) as it contains the species-specific immune gene (IFN-γ) as an adjuvant
Object Sub-Categorization and Common Framework Method using Iterative AdaBoost for Rapid Detection of Multiple Objects
Get PDFObject detection and tracking in real time has numerous applications and benefits in various fields like survey, crime detection etc. The idea of gaining useful information from real time scenes on the roads is called as Traffic Scene Perception (TSP). TSP actually consists of three subtasks namely, detecting things of interest, recognizing the discovered objects and tracking of the moving objects. Normally the results obtained could be of value in object recognition and tracking, however the detection of a particular object of interest is of higher value in any real time scenario. The prevalent systems focus on developing unique detectors for each of the above-mentioned subtasks and they work upon utilizing different features. This obviously is time consuming and involves multiple redundant operations. Hence in this paper a common framework using the enhanced AdaBoost algorithm is proposed which will examine all dense characteristics only once thereby increasing the detection speed substantially. An object sub-categorization strategy is proposed to capture the intra-class variance of objects in order to boost generalisation performance even more. We use three detection applications to demonstrate the efficiency of the proposed framework: traffic sign detection, car detection, and bike detection. On numerous benchmark data sets, the proposed framework delivers competitive performance using state-of-the-art techniques
Network Path Optimization Strategy using Collaborative Cache for Delay Tolerant Networks
Get PDFThe data transmissions over Delay Tolerant Networks (DTN) and Social-based Opportunistic networks have increased in the last few years due to a higher demand for remote transmissions. The wide applications of DTN have significantly motivated the researchers to focus on finding optimized routing strategies by optimizing various parameters such as energy, cost and congestion. Nonetheless, these parallel research outcomes have reported few further bottlenecks to improve the strategies. Henceforth, this work proposes a novel approach to optimize the routing paths using the cache collaboration method. This proposed method identifies the data-sharing strategies and subsequently identifies the sub-set of the paths between the source and destinations. Further optimizes the path using standard measures such as cost, transmission speed, and the network traffic conditions; lastly Data-Centric and Cost Optimized Routing Path is Identification. This work results in a nearly 20% reduction in the distance between the nodes, a 15% reduction of time in path identification and a nearly 50% reduction in cache allocation demand over multiple iterations compared to the existing models
Anticancer potential of Solanum lycopersicum L. extract in human lung epithelial cancer cells A549
Get PDFThe study aimed to reveal the phytochemical profile, free radical scavenging potential, and anticancer activity of Solanum lycopersicum L. leaf extract (SLLE). According to the study, SLLE contains plant secondary metabolites that are beneficial for health, like phenolics, flavonoids, ascorbic acid, alkaloids, and terpenoids. The SLLE has shown potential free radical scavenging potential in DPPH and ABTS free radical scavenging analysis and its EC50 values (concentration required to inhibit 50% of free radicals) were determined as 481.29 ± 33.82 and 527.56 ± 20.34 µg/mL, respectively. The SLLE has the ability to scavenge free radicals and could be used to treat illnesses brought on by oxidative stress. The anticancer activity of SLLE was assessed by MTT, LDH, micro-morphological, live/dead dual staining, and caspase-3 analysis. In the MTT assay, the IC50 value (concentration required to inhibit 50% of cell viability) of SLLE was determined as 190.41 ± 4.77 µg/mL. Furthermore, SLLE has shown potential anticancer activity by adversely affecting the plasma membrane integrity and escalating the caspase-3 levels. In the biomedical field, SLLE could be highly useful to treat cancer
Multiphysics Experimental Approaches for Insight into the Hydrogen Bonded Structures of Ethylene Glycol and Glycerol Mixtures toward Green Solvent Technology
Get PDFAlcohols and their mixtures are credited most important polar solvents for advances in pharmaceutical, chemical,biological, thermal, and material technologies. A rigorous study for the characterization of hydrogen bonded heterogeneousintermolecular structures that formed in a mixed solvent based on alcohols containing two and three hydroxyl groupsmolecules is crucial to specific technological and industrial applications. Hence, in this work, the multiphysics experimentalapproaches including the measurements of dielectric, electrical, viscous, acoustic, thermal, and optical properties are appliedand analyzed to confirm the behaviour of hydrogen bonded molecular structures of ethylene glycol (EG; dihydric alcohol)with glycerol (Gl; trihydric alcohol) over the entire concentration range of EG+Gl mixtures at 298.15 K. The static dielectricpermittivity, direct current electrical conductivity, low frequency relaxation time, and refractive index values of the EG+Glmixtures are reported. Additionally, dynamic viscosity, density, ultrasound velocity, adiabatic compressibility,intermolecular free length, acoustic impedance, free volume, Rao’s constant, Wada constant, and viscoacoustic relaxationtime of the EG+Gl mixtures are determined, and also explored their significance to these alcohols molecular interactions.Ultraviolet-visible range absorbance behaviour of the alcohol mixtures is characterized in detail and confirmed theelectronic transitions at higher energy ultraviolet radiations. The detailed analysis of all the experimental results along withthe consideration of excess properties evidenced the formation of heterogeneous intermolecular hydrogen bonded structuresin these Newtonian-type alcohols mixtures. A small to adequate variation in the thermodynamical and other investigatedproperties with the concentration variation showed that the EG+Gl mixture can be optimized as a green solvent according tothe prerequisite properties for huge advances in soft condensed matter technologies
Carboxymethylated gum tragacanth crosslinked poly(sodium acrylate)hydrogel: Fabrication, characterization, rheology and drug-delivery application
Get PDF This study aimed to synthesize Carboxymethylated Gum Tragacanth (CMGT) from the Gum Tragacanth (GT). Modified gum’s potential as a drug delivery carrier has been explored by synthesizing CMGT based hydrogels. The viscoelastic characteristics of the fabricated hydrogel have been examined by Rheological analysis. SEM studies have been conducted to examine the surface morphology and TGA is used to perform the thermal analysis. XRD, FTIR, and solid state 13C NMR have been used to examine the structural features of the GT, CMGT, sodium acrylate based hydrogel (SAH) and carboxymethylated gum tragacanth co-polymerized with sodium acrylate based hydrogel (CMGT-co-SAH). The anti-inflammatory drug, Aceclofenac Sodium(AFS) loaded onto the hydrogel has been used as a model drug. The AFS follows the Fickian mechanism of diffusion and the results are best fitted in Higuchi and Korsmeyer–Peppas models.
Problems of Enforcement of Patent Law in China and Its Ongoing Fourth Amendment
Get PDFWith respect to the enforcement of the patent law in China, the United States and the EU both contend that the principal issue is more related to the weakness in enforcement than the legislations. Chinese experts believe that major problems in China’s patent law implementation features “difficulty to prove, long cycle length, high cost, low compensation, and poor result”. To solve those problems, China started the fourth amendment to its Patent Law in November 2011. Up until now, the draft of the revised Patent Law is still in review. If this amendment is approved, the enforcement of the Patent Law in China is expected to be enhanced significantly
Mayo v. Prometheus: The Eternal Conundrum of Patentability v. Patent Eligibility
Get PDFIn 2012 the Supreme Court adjudicated a profound medical claim in Mayo Collaborative Services v. Prometheus Laboratories Inc., which has the potential of radically altering patent law and § 101 jurisprudence. The judgment came as a shock to the patent community; however, judgments of the lower courts therewith have echoed similar decisions to show the judgment in Prometheus simply cited Supreme Court jurisprudence. This paper talks about the judgment and its effects on the rulings of the Federal Circuit as well as District Courts, and its effect on medical claims. It gives an insight into patent laws in the United States and where it is headed. While the Federal Circuit remains split as to how narrowly it would construe Prometheus so as to apply it to other cases, its forthcoming opinion en banc on CLS Bank Int’l v. Alice Corp. Pvt. Ltd. is likely to unify its interpretation. What is yet to be seen is how broadly the District Courts will see the judgment so as to judge similar medical claims. The process has already been started, as will be explained in this paper, the effects of which however are too early to be discussed