Vietnam Academy of Science and Technology: Journals Online
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Studying of cyp2c19*2, *3 and *17 polymorphism in Vietnamese patients with coronary artery disease
No full textCoronary artery disease is the most common type of cardiovascular disease, due to the accumulation of atherosclerotic plaque inside the arterial wall which leads to block blood supply to the heart muscle. A number of clinical trials have demonstrated that Clopidogrel is able to inhibit platelet aggregation in patients with acute coronary syndromes, reduce mortality and cardiovascular events. However, the antiplatelet effectiveness of Clopidogrel significantly depends on CYP2C19 genotypes. Therefore, the aim of this study was to identify the CYP2C19*2, *3 and allele frequencies in Vietnamese coronary artery patients by using PCR-RFLP method. Total genomic DNA were extracted from peripheral blood of 96 patients diagnosed with coronary artery disease. Thereafter, single nucleotide polymorphism sites in the CYP2C19 gene were identified by PCR with specific primers. The amplified products were then digested by restriction enzymes SmaI, BamHI, and MnlI, respectively. The results showed that the proportion of heterozygous individuals for CYP2C19*2 (c. 681GA, rs4244285), CYP2C19*3 (c. 636GA, rs4986893), and CYP2C19*17 (g. -3402CT, rs11188072) accounted for 39.58%, 6.25%, and 2.08%, respectively. Among 96 subjects, 41.67% of patients were predicted for intermediate metabolic phenotype CYP2C19*1/*2 (37.50%) and CYP2C19*1/*3 (4.17%). Approximately 10.42% of total patients represented poor metabolizers in which 8.34% had two copies of the same allele *2/*2 and 2.08% had *2/*3 genotype. Particularly, two individuals (2.08%) detected with CYP2C19*1/*17 genotype were able to increase CYP2C19 activity (ultrarapid metabolizers). The results of this study generate a foundation for introducing individualized antiplatelet therapy in Vietnam based on genetic testing
Promising activity against NTERA-2 cancer stem cells of malloapelta B compound isolated from the Bum-bup plant in Vietnam
No full textRecent studies have revealed that cancer stem cells (CSCs) drive tumor growth, metastasis, therapy resistant and recurrence. Therefore, CSCs are considered as new targets for screening of more effective drugs. The compound malloapelta B, which was isolated from the Bum-bup plant (Mallotus apelta) (Lour.) Muel.-Arg, Euphorbiaceae) in Vietnam, presented very potent and strong anticancer invitro, especially against the activation ofNF-kB (nuclear factor- kappa B). In our research, malloapelta B inhibited the growth of NTERA-2 line, a CSC line, at the value of IC50 = 12.71 ± 0.76 µM. In this study, malloapelta B also had effects on cell cycle of NTERA-2 by significantly reducing the cell number at G0/G1 phase (37.48%), while raising this number of phase G2/M to 31.12% in comparison with those of control (56.81% and 18.6%, respectively). Besides, clonogenic and tumorspheroidal formations are acknowledged as typically renewable characteristics of CSCs. Then, in our studies, malloapelta B significantly prevented NTERA-2 to form clonogens and reduced tumorspheres’ sizes at treatments with 20 and 100 µg/mL. However, malloapelta B did not exhibit any obvious effects on the expression level of CD44+/CD24+, the two typical cell surface markers of CSCs, at 2.5 and 5 µM treatments
Evaluation of growth and carcass performance of crossbred F1 between wild boar from Central Highland with Mong Cai and Soc pigs
No full textThis study aimed to evaluate the growth and meat productivity of F1 crossbreds between Central Highland wild boards with Mong Cai and Soc pigs. The experiment was conducted on 2 groups of F1 pigs including F1 crossbred between native wild boars and Mong Cai pigs (RxM), F1 crossbred between of native wild boars and Soc (RxS) pigs, and 2 groups of Mong Cai pigs (M) and Soc pigs (S). The results demonstrated that the growth of F1 pig of RxM group was higher than RxS group after 3 months and 8 months of breeding. The carcass analysis showed that there was no difference in the slaughter weight of pigs between the RxM and M groups, as well as between the RxS and S groups. Pigs from the S group showed the lowest hanging weight (17.60 ± 0.52 kg) and lowest dressed weight (14.92 ± 0.49 kg) among the groups. There was no difference in the hanging weight and dressed weight among the RxM and M groups. However, the ratio of hanging weight (75.95 ± 0.23%) and dressed weight ratio (65.00 ± 0.18%) of RxM group is higher than M group (72.53 ± 0.44% and 62.24 ± 0.51%, respectively). The RxS group also had a higher percentage of hanging weight (74.28 ± 0.57%) and dressed weight (63.73 ± 0.48%) than the S group (71.10 ± 0.40% and 60.25 ± 0.50%, respectively). In this study, we also found that F1 hybrid pigs exhibited a lower backfat thickness than the M and S groups. In contrast, the backskin thickness increased in F1 hybrid groups. The above results showed that the hybridization of with Mong Cai and Soc pigs with Central Highland wild boars could improve the meat productivity of their F1 generation
Evaluation of the sperm separability of blanc-blue-belge bull by swim-up method and in vitro embryo production with hybrid Zebu bovine oocyte in Vietnam
No full textThe objective of this study was to determine the right conditions for the separation of Blanc-Blue-Belge bovine sperm (BBB) by swim-up mothed; determine the maturity of hybrid Zebu bovine eggs; and culture of embryos after in vitro fertilization. After 60-80 minutes of swim-up in CAP-05, BBB bovine sperms were healthy, straight movement and separated with a concentration of 106 sperm/ml. Hybrid Zebu bovine eggs developed and matured in the maturation medium with the basic medium TCM-199 supplemented with 10% calf serum, FSH (0.75 µg / ml), LH (0.15 µg / ml) and Estradiol (2.5 µg / ml), the results showed that the IVM-08 medium had significantly higher maturation rates than IVM-03, the proportion of mature eggs reached 71,11% compared to 51.69%, respectively (P 0.01). In vitro fertilization of hybrid Zebu bovine egg in IVF-08 medium. In vitro fertilized embryos (BBB x hybrid Zebu) developed from bovine sperms separated by the swim-up method achieved a better rate of morula-blastocysts in IVC-09 than IVC-06 medium, 21.68% compared to 8.56%, respectively (P 0.01). The conclusion was that the suitable conditions for BBB bovine sperm separation and in vitro embryo production (BBB x hybrid Zebu) were determined. This is the premise to create bovine semen, BBB bovine embryos with defined gender
STRUCTURAL, MAGNETIC AND ELECTRICAL PROPERTIES OF Ho AND Ni CO-DOPED BiFeO3 MATERIALS
Get PDFPure BiFeO3 (BFO) and Bi1-xHoxFe0.97Ni0.03O3 (x = 0.00, 0.025, 0.05, 0.075, and 0.10) materials were synthesised by sol-gel method. Effects of (Ho, Ni) co-doping on structural, magnetic and electrical properties of BFO materials were investigated by different techniques as X-ray diffraction (XRD), energy dispersion X-ray (EDX), Raman scattering, magnetic hysteresis (M-H) loops, and complex impedance spectra measurement. Analysis results of XRD measurement shows that all samples crystallize in the rhombohedral structure with R3C space group. BFO in the rhombohedral structure has crystal lattice parameters a = 5.584 Å, c = 13.867 Å and average crystal size LXRD = 60 nm. The x = 0.00 sample (BFNO) has a = 5.589 Å, c = 13.875 Å and LXRD = 60 nm. However, the crystal lattice parameters a, c and average crystal size LXRD of (Ho, Ni) co-doped samples decrease when concentration of Ho increases. Results of Raman scattering spectra shows that the peaks position characteristic for Fe-O bonds of x = 0.00 sample shifts toward lower frequency compare to that of BFO. For the (Ho, Ni) co-doped samples (BHFNO), the peaks position characteristic for Bi-O convalent bonds shifts toward higher frequency when concentration of Ho increases. Which confirmed that Ho3+ and Ni2+ ions substituted into Bi-sites and Fe-sites, respectively. From data of magnetic hysteresis loops measurement indicates that all samples present weak ferromagnetic. BFO sample presents weak ferromagnetic properties with saturation magnetization Ms = 0.047 emu/g and remnant magnetization Mr = 0.008 emu/g. Ferromagnetic properties of (Ho, Ni) co-doped samples enhance compare to that of BFO. In this report, we will discuss the origin of ferromagnetism of materials
FREE VIBRATION ANALYSIS OF POROUS NANOPLATES USING NURBS FORMULATIONS
Get PDFThis paper presents free vibration analysis of functionally graded (FG) porous nanoplates based on isogeometric approach. Material properties of the nanoplates are given by using a modified power-law function. The Eringen’s nonlocal elasticity is used to capture the size effects. Based on a combination of the Hamilton’s principle and the higher order shear deformation theory, the governing equations of the porous nanoplates are derived. The obtained results demonstrate the significance of nonlocal parameter, porosity volume fraction, porosity distributions and volume fraction exponent on free vibration analysis of the nanoplates with porosities
Chemical composition and antibacterial activities of essential oils from fruits of Melicope pteleifolia (Champ. Ex Benth.) T.G. Hartley grown in Lam Dong Province, Vietnam
Get PDFIn the present study, chemical composition and antibacterial properties of essential oil obtained from the aerial parts of the Melicope pteleifolia (Champ. ex Benth.) T.G Hartley in Dalat were evaluated. Essential oil was isolated through hydro-distillation. Twenty-nine constituents comprising 100% of the essential oil were characterized by gas chromatography/mass spectrometry (GC-MS) techniques. The major compounds in the essential oil were (+)-Sabinene (34.73%), Cis-α-bergamotene (13.15%), Z-α-trans-bergamotol (5.28%), β-mycrene (4.98%), and 1,3,6-octatriene, 3,7-dimethyl-(4.71%). Antibacterial activities of Melicope pteleifolia essential oil were investigated against Gram-positive and-negative bacteria. Results showed significant activities against Streptococcus pyogenes and Escherichia coli using an agar well diffusion method. The application of this essential oil in preventing and eliminating bacteria could be useful in fields as medicine and cosmetics.
Personalized medicine for effective treatment of non-small-cell lung cancer with targeted therapies
No full textLung cancer is the most common cause of cancer death worldwide, with most deaths having distant metastases. It has become increasingly complex to get effective treatment for lung cancer patients. While generalized medicine with traditional therapy resulted in comparatively poor response, personalized medicine has been well known to be an important strategy for effective treatment of lung cancer, with current focus on significant detection of clinical oncogenic drivers responsible for tumor initiation and maintenance and development of drug resistance. In lung cancer, especially in non-small-cell lung cancer (NSCLC), EGFR, ALK, RET, ROS1, BRAF, KRAS, NRAS, PIK3CA, DDR2, MET, ERBB2 have been reported to be key oncogenic drivers, which are targeted in the development and application of targeted therapeutic drugs. Personalized medicine based on these oncogenic drivers is highly recommended for treatment of advanced NSCLC patients. In this article, the significant application of personalized medicine based on the key oncogenic drivers for effective treatment of NSCLC with targeted therapeutic drugs is reviewed.
Mutations in NPHS1 and PLCE1 (NPHS3) in two Vietnamese patients with conginetal nephrotic syndrome
No full textCongenital nephrotic syndrome (CNS), a genetic disease caused by the mutations in genes on autosomes,is usually occurs in the first three months after birth. The mutations in genes that encode for the structural andfunctional proteins of podocytes lead to loss of the function of glomerular filtration. So far, a number of genesrelated to the disease have been identified such as: NPHS1, NPHS2, PLCE1 (NPHS3), ACTN4, CD2AP, INF2and WT1. In this article, we amplified all of exons in NPHS1 and PLCE1 genes of two Vietnamese patientswith CNS and members of patients’ family. PCR products were purified and sequenced directly on automaticsequencer ABI 3500 Bio System (USA). The sequencing results were compared with the sequences of NPHS1and PLCE1 genes published in the Ensembl database (ENSG00000161270 and ENSG00000138193,respectively) by using BioEdit software to detect mutations. We identified two mutations: c.2398CT(p.Arg800Cys, exon 18), c.3315AG (p.Ser1105Ser, exon 26) in NPHS1 gene and two mutations: c.5330 CT(p.Thr1777Ile, exon 23), c.5780AG (p.His1927Arg, exon 25) in PLCE1 gene in study patients. These twopatients carried simultaneously the mutations in the NPHS1 and PLCE1 genes with serious phenotype. Theresults of our study might be evidences for the role of mutations in NPHS1 and PLCE1 genes in thedevelopment of disease in patients. These are useful information in identifying the cause of disease and providethe genetic counseling to the patients’ family
Effects of salinity and shaking condition on the growth and virulence of Vibrio parahaemolyticus
No full textVibrio parahaemolyticus is a Gram-negative halophilic bacterium that is found in estuarine, marine and coastal environment. This organism is the major causative agent of Early Mortality Syndrome (EMS) or Acute Hepatopancreatic Necrosis Disease (AHPND) which resulted in serious damages to cultured shrimp industry. Understanding the effect of environmental factors on the growth and virulence of this potential pathogen would be beneficial for preventing its outbreak. In this study, the growth and virulence of V. parahaemolyticus was examined under different salinity and shaking condition. V. parahaemolyticus XN9 was cultured in Brain Heart Infusion (BHI) medium with different sodium chloride concentrations (2.0, 2.5 and 3.0%) and different shaking conditions (0, 120 and 240 rpm). The growth of the bacterium was recorded over 8h and six extracellular enzymes of V. parahaemolyticus XN9 including caseinase, hemolysin, lecithinase, lipase, gelatinase, chitinase were investigated using agar-based method. The growth of V. parahaemolyticus was varied among different salinity and shaking conditions. It showed the best growth at 2.0% NaCl and 240 rpm. No change in the enzymatic activity (EA) of the tested extracellular enzymes was observed while changing salinity except the significant decline of gelatinase from 3.49±0.19 to 2.77±0.17 mm following salinity increase (p 0.05). On the other hand, regarding shaking condition, lipase was the one to increase its activity significantly following the increase of shaking speed (p 0.05). While caseinase, lecithinase, gelatinase and lipase were well expressed in V. parahaemolyticus, no hemolytic and chitinase activity was observed in any tested conditions. In summary, our study showed that 2.0 % NaCl and 240 rpm shaking promoted the best growth of V. parahaemolyticus and resulted in highest activity of gelatinase and lipase in this bacterium