Vietnam Academy of Science and Technology: Journals Online
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Genetic testing identifies the potential risk of multiple endocrine neoplasia in a Vietnamese family
No full textMultiple endocrine neoplasia type 2A (MEN2A) is a rare syndrome characterized by the presence of medullary thyroid carcinoma, pheochromocytoma, hyperparathyroidism and sometimes cutaneous lichen amyloidosis. This syndrome is caused by a germline activation mutation in the rearranged during transfection (RET) proto-oncogene transmitted by an autosomal dominant inheritance. In this study, we reported a rare case of a 44-year-old man from Vietnam with medullary thyroid carcinoma and pheochromocytoma as the symptom of MEN2A. Genetic testing indicated a nucleotide substitution located in exon 11 of the RET proto-oncogene (c.1900TC, p.C634R), which was reported as a known pathogenic mutation of MEN2A. Further genetic tests on the other family members found the same mutation in his daughter (currently 14 years-old) and his son (currently 8 years-old). Although these 2 children do not yet have any manifestations of MEN2A, this data emphasizes their high risks of this disease. Therefore, this case draws attention to the importance of genetic counselling in C634R carriers, as well as rigorous follow-up appointments to reduce incidence and mortality since the mutation is classified as a high-risk group within the medullary thyroid carcinoma guidelines
Evaluation of the potential SNPs for breeding selection of white spot syndrome virus resistance in Litopenaeus vannamei
No full textWhite spot syndrome virus (WSSV) is a dangerous virus causing great damage to shrimp farming. Many genes related to disease resistance mechanisms have been identified and studied. In this study, we amplified and sequenced nine fragments of DNA harboring SNPs in the genes involved in WSSV resistance of white-legged shrimp, Litopenaeous vannamei. Allele frequencies at the SNP loci were recorded and calculated by SPSS statistical software (version 22) in the study groups: the disease shrimp (were infected with WSSV and died) and the resistance shrimp (were infected with WSSV but survived). Six SNPs (in AIF, ALF1, HAE, P53, Rab5B, and TRAF6 genes) were in accordance with Hardy–Weinberg Equilibrium (HWE) (p 0.05) while three SNPs (in ALF2, BGB, and CAL) were not (p 0.05). For AIF and ALF1 genes, the frequencies of GG and CC genotypes were significantly different between two groups and were associated with an decreased resistance with WSSV compared to the AA and TT genotypes (p = 0.021 and p = 0.017, respectively). The G and C alleles were associated with a decreased resistance with WSSV (p = 0.000 and p = 0.001, respectively). For HAE gene, the frequency of TT genotype was significantly different between two groups and was associated with a increased resistance with WSSV compared to the TC+CC genotype (OR = 68.750; 95% CI: 11.462–412.381; p = 0.000). For Rab5B gene, the frequency of CC genotype was significantly different between two groups and was associated with an increased resistance with WSSV compared to the TT genotype in all three tested models (p0.05). The C allele was associated with an increased resistance with WSSV (OR = 3.974; 95% CI: 1.754–9.003; p = 0.001). The above evaluation results suggested that the potential SNPs of these AIF, ALF1, HAE, and Rab5B genes can be used as the molecular markers for breeding selection the resistance to white spot disease in white-legged shrimp L. vannamei.
Transgenic soybean overexpressing GmNAC085 enhanced expression of important genes under salinity stress condition
No full textClimate change has made abiotic stresses such as drought and salinity bigger threats to ecosystem and global food security. In response to unfavorable conditions, physiological, biochemical and molecular activities in plants are altered. Particularly, various members of NAC (NAM, ATAF1/2, CUC2) transcriptional factor family have been reported to be the key regulators in modulating multiple biological processes of plant responses to osmotic stress conditions caused by drought and salinity. Previously, transgenic studies have shown the positive regulatory role of GmNAC085, a NAC transcription factor from soybean (Glycine max), in plant resistance against drought stress. Therefore, in this study, we extended the investigation on its contribution to salinity stress. According to our RT-qPCR analyses, expression of several important stress-related genes was significantly induced in the transgenic soybean plants in comparison with the wild-type plants, including antioxidant enzyme-encoding genes (GmSOD, GmAPX and GmCAT), sodium-proton antiporter encoding gene (GmNHX1) and proline metabolic gene (GmP5CS). Furthermore, biochemical results were also in agreement with the molecular data, with enhanced antioxidant enzyme activities of peroxidase and catalase, and in couple with lower cellular content of the reactive oxygen species hydrogen peroxide in the transgenic plants. Taken these altogether, the transgenic plants might acquire advantages in dealing with oxidative stress using enzymes and proline, as well as cellular Na+ removal under salinity stress conditions. Therefore, underlying mechanisms of GmNAC085 associated with salinity stress should be fully elaborated to find out its potential utility in crop improvement
Whole-genome sequencing and de novo assembly of a 2019 novel coronavirus (SARS-CoV-2) strain isolated in Vietnam
No full textThe pandemic COVID-19 caused by the virus SARS-CoV-2 has devastated countries worldwide, infecting more than 4.5 million people and leading to more than 300,000 deaths as of May 16th, 2020. Whole-genome sequencing (WGS) is an effective tool to monitor emerging strains and provide information for intervention, thus help to inform outbreak control decisions. Here, we reported the first effort to sequence and de novo assemble the whole genome of SARS-CoV-2 using PacBio’s SMRT sequencing technology in Vietnam. We also presented the annotation results and a brief analysis of the variants found in our SARS-CoV-2 strain, which was isolated from a Vietnamese patient. The sequencing was successfully completed and de novo assembled in less than 30 hours, resulting in one contig with no gap and a length of 29,766 bp. All detected variants as compared to the NCBI reference were highly accurate, as confirmed by Sanger sequencing. The results have shown the potential of long read sequencing to provide high quality WGS data to support public health responses and advance understanding of this and future pandemics
Inhibited growth of mesenchymal stem cells under simulated microgravity
No full textThis study aimed to estimate the effects of simulated microgravity (SMG) on mesenchymal stem cells (MSCs). The 3D clinostat was applied to induce to simulated microgravity on MSC. The results showed that the MSC density in control group was higher than the SMG group. The cell cycle analysis demonstrated that the MSC ratio in G0/G1 phase in SMG group was higher than that of the control group, while the MSC ratio in S phase and G2/M phase in SMG group was lower than those of the control group. The real time quantitative RT-PCR was used to evaluate the expression of cell cycle-related genes, including Cyclin-dependent kinase 2 (Cdk2), Cyclin-dependent kinase 6 (Cdk6), and Cyclin A. The results showed that the transcript expression of Cdk2, Cdk6, and Cyclin A was down-regulated in MSC from SMG group comparing to that of the control group. The flow cytometry was performed to determine the ratio of apoptotic MSCs. There was no significant difference in viability ratio and apoptotic ratio of MSCs between SMG group and control group. The MSCs from SMG group and control group showed similar in Bcl2 and Bax transcript expression
Interactionsbetween of Orius sp. (Heteroptera: Anthocoridae) and Amblyseius (Typhlodromips) Swirskii (Athias-Henriot) (Acari: Phytoseiidae) for biological control of Thirps palmi karny (Thysanoptera: Thripidae) on muskmelon
No full textIn this study, we examined biological control Thirps palmi Karny of a predatory mite Amblyseius (Typhlodromips) swirskii (AthiasHenriot), the anthocorid bug Orius sp. and combination of Amblyseius swirskii and Orius sp. The experiments in laboratory condition showed that combination of these two species have the ability to prey consumtion is higher than using only predator (Orius sp. or Amblyseius swirskii). The fecundity of Orius sp. in the absence of Amblyseius swirskii (the total number of eggs/ females = 45.5 ± 2.25 eggs, the oviposition period = 16.49 ± 0.5 days) were higher than in the presence of Amblyseius swirskii (the total number of eggs/ females = 35.6 ± 2.33 eggs, the oviposition period = 13.1 ± 0.63 days). Similar with Amblyseius swirskii, the fecundity of Amblyseius swirskii in the absence of Orius sp. (the total number of eggs/females = 35.57 ± 3.62 eggs, the oviposition period = 21.33 ± 0.7 days) were higher than in the presence of Amblyseius swirskii (the total number of eggs/ females = 24.1 ± 1.67 eggs, the oviposition period = 13 ± 1.43 days). In the greenhouse with area of 300m2, the experiments were conducted when used two-predator were average aduts (Orius sp. were 3.47 adult/ leaf, A. swirskii were 6.96 adult/ leaf) were aqual as release of only Orius sp. (average adult = 3.81 adult/ leaf) or only Amblyseius swirskii (average adult = 6.49 adult/ leaf). This study also provides further evidence that intraguild predation does not necessarily have negative effects on biological control
Screening of antihyperglycaemic activity of medicinal plant extracts instreptozocin-induced type 2 diabetic mice
No full textTraditional plant used for treatment of diabetes has shown a surging interest in the last few decades. The purpose of this study is to evaluate the effect of 70% (v/v) ethanolic extracts of several untested herbal sources, such as papaya leaves, papaya seeds, fig fruits, fruits of Xanthium strumarium, and leaves of Gomphrena celosioides on blood glucose levels of streptozocin (STZ)-induced type 2 diabetic mice. The results proved that diabetic mice treated with the extracts of papaya seeds, papaya leaves and fig fruits showed significant reduction of the blood glucose levels at a dose of 500 mg/kg body weight when compared to control. Antihyperglycemic activity of the papaya leaves (52.38%) and papaya seeds extracts (53.16%) were significantly higher than the extract of fig fruits (27.90%). Furthermore, the α-amylase inhibition assay showed that the extracts of papaya seeds (58,63 ± 0,06 μg/ml), papaya leaves (34,28 ± 0,06 μg/mL) and fig fruits (79,24 ± 0,83 μg/mL) exhibited 50% α-amylase inhibition activity at the mentioned concentrations. The α-glucosidase IC50 of the extracts of the papaya seeds, papaya leaves and fig fruits were found to be 24.36 ± 0,29, 27,60 ± 0,43 and 106,44 ± 1,54 respectively. The results of the study indicate the potential of these extracts to manage hyperglycemia. The histopathological study of the papaya seeds extract has shown to ameliorate the streptozocin-induced histological damage of islets of Langerhans in the pancreas
The high-throughput screening system for inhibitor Mycobacterium tuberculosis compounds based on ATP hydrolysis activity of recombinant protein ClpC1
No full textThe global Tuberculosis (TB) rate continues to increase by 1% per year with the widespread of drug-resistant TB. Therefore, the development and research to find new anti-TB drugs are becoming an extremely urgent mission. To be able to screen lead anti-tuberculosis drugs, currently, researchers have to carry out directly on the cells of Mycobacterium tuberculosis and to be performed in bio-security facilities level 3 or 4, to prevent infection from pathogens. However, our results demonstrated that the screening of anti-TB drug candidates can be implemented in bio-security facilities level 1 laboratory with the Escherichia coli cell extraction and recombinant ClpC1 protein - an integral part of the Mycobacterium tuberculosis genome. We focused on the ATP hydrolysis activity of ClpC1 to create a specific research direction for the high-throughput anti-tuberculosis drug screening system. ClpC1 protein was overexpressed and purified with functionally characterized (93.5 kDa). The steady-state growth of recombinant ClpC1 protein in Luria-Bertani (LB) broth High Salt medium was maintained and stabilized after extraction. The determined ATPase activity of ClpC1 was performed by measuring the released phosphate from the reaction. Ecumicin was chosen to be a control compound with expected ATP hydrolysis activities (Hill coefficient = 1,19 ± 0,217; Kd value = 0,52 ± 0,275). We tested this high throughput screening system with ten anti-TB drugs to evaluate the effectiveness of our screening system. Based on these results, we built a complete high-throughput screening system anti-tuberculosis drug safety and quickly
WATER QUALITY ASSESSMENT OF SAIGON RIVER FOR PUBLIC WATER SUPPLY BASED ON WATER QUALITY INDEX
No full textWater Quality Index (WQI) is a dimensional number that aggregates information from many water quality parameters according to a defined method. WQI is accepted as an efficient tool for water quality management. In this study, WQI of Saigon river for public water supply was calculated from nine water quality parameters including pH, suspended solids (SS), dissolved oxygen (DO), chemical oxygen demand (COD), nitrite, ammonia, phosphate, total dissolved iron and total coliform based on water quality data obtained monthly from January 2016 to December 2019 at three sampling sites. The results showed that most of WQI values belonged to class III (medium water quality with the WQIs of 35 – 64) and class IV (poor water quality with the WQIs of 11 – 34) and a deteriorating trend was observed from upstream to downstream of Saigon river. The river water quality could not be used for public water supply
PTP1B INHIBITORS FROM ISODON TERNIFOLIUS COLLECTED IN VIETNAM
No full textFrom the whole plant of Isodon ternifolius collected in Vietnam, four triterpens including ursaldehyde (1), ursolic acid (2), b-sitosterol (3) and b-sitosteryl ferulate (4) were purified. Their chemical structures were determined by interpretation of NMR and MS data and comparison with the literatures. Compounds 1-4 were evaluated for their inhibitory activity against PTP1B enzyme activity using in vitro assay. Compounds 1 and 2 displayed potential activities with IC50 values of 16.92 ± 0.12 and 3.42 ± 0.45 μM, respectively. This is the first time that compounds 1 and 4 have been isolated from the Isodon genus and I. ternifolius has been evaluated for the PTP1B inhibitory activity