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ANTIOXIDANT ACTIVITY OF FRUITING BODY EXTRACTS FROM Pycnoporus sanguineus (L.: Fr.) Murrill MUSHROOM
No full textThe objective of the this study is to provide an in vitro evidence for the potential antioxidant activity of the ethanolic and aqueous extracts from fruiting bodies of Pycnoporus sanguineus mushroom via DPPH free radical scavenging and lipid peroxidation inhibition assays. DPPH free radical and lipid peroxidation inhibitory activities of ethanolic and aqueous extracts from fruiting bodies of P. sanguineus were examined in a dose-response manner. Ascorbic acid and trolox were was used as a positive control for these assays and the results showed that the ethanolic extract of P. sanguineus possessed strong inhibitory activity on DPPH free radical and lipid peroxidation with IC50 values of 196.68 and 975.84 μg/mL, respectively. The aqueous extract also exhibited moderate activity against DPPH free radical and lipid peroxidation with IC50 values of 322.03 and 1311.24 μg/mL, respectively. Which was significantly lower than that of ascorbic acid and trolox with IC50 values of 55.00 and 886.68 μg/mL, respectively. The degree of DPPH free radical and lipid peroxidation inhibition was correlated with the dose of inhibitors. Based on the obtained results, the P. sanguineus mushroom possesses high potential in antioxidant activity and could be a potential source of natural antioxidants in food and pharmaceutical applications
NGHIÊN CỨU MÂU THUẪN VÀ LỰA CHỌN ƯU TIÊN TRONG QUY HOẠCH TỔNG HỢP KHÔNG GIAN VEN BIỂN HUYỆN HẢI HẬU - NGHĨA HƯNG TỈNH NAM ĐỊNH
Full text linkHai Hau - Nghia Hung are two coastal districts in the Southeast of Nam Dinh province with total area of 35,652.29 km2. Located in the middle between the Southern provinces of the Red river delta and the North Central provinces, Hai Hau and Nghia Hung are about 100 km from Hanoi along National Route 1A and 80 km from Hai Phong, in an area directly affected by the Hanoi - Hai Phong - Quang Ninh growth triangle. The two districts have an approximately 47 km coastline accounting for over 65% of the coastline of the province. Along the coastline there are four rivers: Red river, So river, Ninh Co river and Day river emptying into the sea through the estuaries: Ba Lat, Ha Lan, Ninh Co and Day, which facilitate the development in industry, agriculture, sea ports, trade, tourism... This is also a region with high-speed economic development and a series of plannings. Thus, a number of conflicts in exploiting and using natural resources and environmental protection have been raised, leading to destroyed sceneries, lost ecological balance, and becoming an anxious problem. This paper presents experimental results in defining the conflicts and priorities between sectors and economic fields in integrated coastal space planning of Hai Hau - Nghia Hung districts to select the economic sectors that have the most potential and advantages. Therefore, it will be appropriate in space usage in exploiting and using natural resources and environmental protection, which will provide the basis for the development of a sustainable marine economy.Hải Hậu - Nghĩa Hưng là huyện ven biển nằm ở phía Đông Nam của tỉnh Nam Định có tổng diện tích tự nhiên 35.652,29km2; Nằm ở vị trí trung chuyển giữa các tỉnh phía Nam đồng bằng sông Hồng với các tỉnh Bắc Trung bộ và trong vùng ảnh hưởng trực tiếp của tam giác tăng trưởng Hà Nội-Hải Phòng-Quảng Ninh, Hải Hậu - Nghĩa Hưng chỉ cách Hà Nội theo quốc lộ 1A gần 100 km và cách Hải Phòng trên 80 km; với đường bờ biển dài khoảng 47km chiếm trên 65% chiều dài bờ biển toàn tỉnh; dọc chiều dài đường biển có 4 con sông: Sông Hồng, sông Sò, sông Ninh Cơ và sông Đáy đổ ra biển qua các cửa sông: Ba Lạt, Hà Lạn, Ninh Cơ và cửa Đáy thuận lợi cho phát triển công nghiệp, nông nghiệp, cảng biển, thương mại, du lịch,….đây cũng là khu vực có tốc độ phát triển kinh tế nhanh với hàng loạt quy hoạch nên khu vực này đang nảy sinh các xung đột gây nên những mâu thuẫu trong khai thác sử dụng tài nguyên thiên nhiên và bảo vệ môi trường dẫn đến cảnh quan bị phá vỡ, cân bằng sinh thái bị mất đi và đang trở thành vấn đề đáng quan ngại. Bài báo trình bày kết quả xác định thử nghiệm những vấn đề mâu thuẫn, ưu tiên giữa các nhóm ngành, lĩnh vực kinh tế trong quy hoạch tổng hợp không gian ven biển huyện Hải Hậu - Nghĩa Hưng nhằm lựa chọn các lĩnh vực kinh tế có tiềm năng, thế mạnh để tổ chức một cách hợp lý trong sử dụng không gian trong khai thác, sử dụng tài nguyên thiên nhiên và bảo vệ môi trường tạo cơ sở cho phát triển một nền kinh tế biển bền vững
Microfluidic Chip for Trapping Magnetic Nanoparticles and Heating in Terms of Biological Analysis
Full text linkIn this study, we reported the results of the design and the fabrication a planar coil in copper (square, a = 10 mm, 15mm high, 90 turns), these planar coils were integrated in a microfluidic chip for trapping magnetic nanoparticles and local heating applications. A small thermocouple (type K, 1 mm tip size) was put directly on top of the micro-channel in poly(dimethyl-siloxane) in order to measure the temperature inside the channel during applying current. The design of planar coils was based on optimizing the results of the magnetic calculation. The most suitable value of the magnetic field generated by the coil was calculated by ANSYS® software corresponded to the different distances from the coil surface to the micro-channel bottom (magnetic field strength Hmax = 825 A/m). The magnetic filed and heating relationship was balanced in order to manipulating the trapping magnetic nanoparticles and heating process. This design of the microfluidic chip can be used to develop a complex microfluidic chip using magnetic nanoparticles
Current status of asian elephant population Elephas maximus in Pumat National Park Region, Nghe An Province
Full text link Asian elephants are under high threats of extinction in Vietnam and worldwide. Population assessment of Asian elephants in Pumat National Park (NP) region, carried out in 2018−2019, showed that the wild Asian elephants population this region consisted of 3 groups with a total of 13−14 individuals. In Tuong Duong District, a group of 4-5 individuals lived in an area near Canh Ta stream (Tam Dinh commune, Vietnam) and Nam Xi stream (Muong Long commune, Laos), across the border between Vietnam and Laos. The group in Con Cuong district, with only one adult female, lived in forest compartments 780, 781,789 and 786 of Con Cuong Forestry Company, within the buffer zone of Pumat NP. The group in Anh Son district consisted of 8 individuals (5 adult females, 1 adult male, 1 sub-adult male and an juvenile born in 2016). They live in Cao Veu sector of Pumat NP core zone and buffer zone areas consisting of protected forests, rubber plantation forests and agricultural lands of households in Phuc Son Commune (Anh Son District) and Thanh Duc Commune (Thanh Chuong District).Wild Asian elephants in Pumat NP region are facing dangers of extinction due to their small number as well as direct and indirect threats from human. The elephant group in Anh Son district, with the largest number of individuals consisting of breeding males, breeding females and a calf, is the most important for the maintainance and future development of elephant population within Puman NP. However, they are facing a number of serious threats such as: un-controlled wildlife hunting and trade; rapid decrease and degradation of natural forest outside of Pumat National Park; and increasingly serious human-elephant conflicts.
An updated list of monogenoidea from marine fishes of Vietnam
Full text linkIn this paper, we updated the list of monogenean species from marine fishes of Vietnam. Taxonomic position of monogenean species were arranged according to the current classification system. A total of 220 monogenean species from 152 marine fish species were listed. Distribution, hosts and references of each species were given. In addition, amendations of taxonomic status of taxa were also updated.
Investigating the genetic variability of 12 Vietnamese rice accessions (Oryza sativa L.) in response to phosphorus deficiency
Full text linkPhosphate is the second major factor limiting on crop productivity and leads to various physiological disorders that would consequently affect plant development. In response to phosphate starvation, plants have to improve their root systems for efficiently acquired phosphate. In Vietnam, rice is one of the most important agricultural crops in which 60% of the rain-fed lowland rice is cultured on soil types, which are low in phosphorus or phosphate fixing. This fact along with the scenario in which phosphate resources are running out in fast pace, highly provoked an indispensible need for developing new rice varieties with high productivity under low phosphate condition. An emerging approach in order to accomplish this goal is through genetic improvement of local rice resources. Taking advantage of that, in this study, out of 182 sequenced Vietnamese rice accessions, 12 representatives from 3 distinguishing rice groups: Indica, Japonica and admix Indica subgroup were chosen. The variation in number of crown root, root length, shoot length, root mass, shoot mass, chlorophyll content and root anatomy were examined as parameters to analyze the effect of phosphorus deficiency (1 µM) compare to full phosphate medium (300 µM). The minimum number of plantlets used for each condition was 15. Each genotype was grown in Yoshida hydroponic medium (changed every 4 days, harvesting was made after 14 days). The experimental results showed a remarkable variation of different rice accessions in response to phosphate deficiency. The starvation of phosphate significantly affected almost all examined traits in which several accessions such as G38, G93, G165, G223, G62 accessions were potential hypersensitive and G11, G177 accessions were potential tolerant to P deficiency. These results are interesting and encouraging to further research for screening our Vietnamese rice collection in order to identify the set of potential genes responsible for tolerance in phosphorus starvation condition
Stimulation of shoot regeneration through leaf thin cell layer culture of Passiflora edulis Sims.
Full text linkPassiflora edulis Sims. belonged to the genus Passiflora, is one of the important economic crops of the world as well as Vietnam. Nowadays, the commercial P. edulis is mainly propagated by seeds, cuttings and grafting; however, these methods still have some limitations such as genetic degradation and heterogeneity and the spread of pathogenic viruses. Micro-propagation has been used for clonal breeding and disease-free plant breeding, as well as providing a source of materials for Passiflora breeding. In this study, leaf explants of P. edulis Sims. (2.0-month-old) excised from the in vitro culture of ex vitro axillary buds cut longitudinally and transversally into thin cell layers (lTCL and tTCL) were used as plant materials to evaluate the shoot regeneration. In addition, the effects of explant age and lighting condition on shoot regeneration were also investigated. After 8 weeks of culture, the results showed that shoot regeneration rate (100%) and shoot multiplication coefficient (13.33) of the in vitro leaf-tTCL-4 were higher than those of other treatments and control. The shoot regeneration rate of P. edulis Sims. also varied with the change of explant age. The highest shoot regeneration rate (100%) was obtained from leaf explants of 1.5-month-old shoots after 8 weeks of culture. Moreover, the light (fluorescent lamps with photoperiod of 16 hours/day and lighting intensity of 40 - 45 μmol.m-2.s-1) improved not only morphogenesis rate, but also shoot regeneration rate (100%) of leaf explants after 8 weeks of culture. This study provided a novel method for rapid micro-propagation of P. edulis Sims
Complete mitochondrial genome of Dong Tao chicken breed (Gallus gallus domesticus) of Vietnam
Full text linkThe complete mitochondrial genome of Dong Tao chicken breed (Gallus gallus domesticus, Dong Tao) was obtained by PCR and sequencing. The complete mitogenome was 16,783 bp in length, with the nucleotide composition for A, T, C, G was 30.29%, 23.75%, 32.48%, and 13.48%, respectively. The mitogenome of Dong Tao chicken contained a non-coding control region (D-loop), 2 rRNA genes, 13 protein-coding genes, and 22 tRNA genes. Phylogenetic analysis revealed that breed Dong Tao chicken breed was sister-close to G. gallus breed Guangxi, and paraphyletic to G. spadiceus, S. jabouillei, and a range of Chinese indigenous, ie. breeds Rugao and Taoyuan
Vitamin C inhibited FasL-induced apoptotic death of mouse dendritic cells through c-FLIP expression
Full text linkVitamin C (VitC) is a potent antioxidant and contributes as an apoptosis inhibitor by preventing death receptor-triggered caspase 8 activity. Fas ligand (FasL) induces the apoptotic cell death via activation of Fas signaling, which is dependent on the expression level of anti-apoptotic molecule c-FLIP (FADD-like IL-1beta-converting enzyme-inhibitory proteins). The present study addressed the effects of VitC on survival of dendritic cells (DCs), a regulator of innate and adaptive immunity. To this end, mouse bone marrow cells were isolated and cultured to attain bone marrow-derived DCs (BMDCs). The cells were treated with FasL in the presence or absence of VitC. Real time RT-PCR, Western blotting and FACS analysis were performed to determine different hallmarks of DC apoptosis. As a result, FasL treatment resulted in activation of caspase 8 and stimulation of cell membrane scrambling, the effects were supressed when VitC was present in the cell culture or the cells were transfected with FLIP siRNA. In conclusion, VitC prevented FasL-triggered DC apoptosis mediated through the expression of c-FLIP
Using ITS-rDNA and matK gene nucleotide sequences for identification ginseng species in Panax in Phu Xai Lai Leng, Ky Son, Nghe An
No full textDNA barcoding is a useful tool for species identification using standardized genomic DNA fragments. We used DNA barcodes (ITS-rDNA and matK gene) to explore Panax (32 samples collected from Phu Xai Lai Leng mountain and 19 samples collected from medicinal nursery of TH), and to investigate the phylogenetic taxonomy of Panax. In this study, the PCR success rate for ITS-rDNA and matK region was 100%. The success rate of bidirectional sequencing of PCR product was 100% of ITS-rDNA and matK region with length of 616 bp, 1433 bp, respectively. All 32 samples (Panax TB) of Phu Xai Lai Leng have a close relationship with P. stipuleanatus (MLBS = 99%, BPP = 100%). All 19 samples (Panax TH) of medicinal nursery have a close relationship with P. notoginseng (MLBS = 100%, BPP = 100%). Interspecific genetic distances within and among Panax species was varied from 0.2% to 7.9%, average (4%) (ITS-rDNA gene) and 0.1 to 2.9%, average (1.2%) (matK gene). The genetic relationship of species/gender belonging to the Panax genus showed that they have the same evolutionary origin and discovered that new distributed of P. stipuleanatus in Phu Xai Lai Leng mountain in Vietnam