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Pyrosequencing of mcrA and archaeal 16S rRNA genes reveals diversity and substrate preferences of methanogen communities in anaerobic digesters
No full textMethanogenic archaea play a key role in biogas-producing anaerobic digestion and yet remain poorly taxonomically characterized. This is in part due to the limitations of low-throughput Sanger sequencing of a single (16S rRNA) gene, which in the past may have undersampled methanogen diversity. In this study, archaeal communities from three sludge digesters in Hong Kong and one wastewater digester in China were examined using high-throughput pyrosequencing of the methyl coenzyme M reductase (mcrA) and 16S rRNA genes. Methanobacteriales, Methanomicrobiales, and Methanosarcinales were detected in each digester, indicating that both hydrogenotrophic and acetoclastic methanogenesis was occurring. Two sludge digesters had similar community structures, likely due to their similar design and feedstock. Taxonomic classification of the mcrA genes suggested that these digesters were dominated by acetoclastic methanogens, particularly Methanosarcinales, while the other digesters were dominated by hydrogenotrophic Methanomicrobiales. The proposed euryarchaeotal order Methanomassiliicoccales and the uncultured WSA2 group were detected with the 16S rRNA gene, and potential mcrA genes for these groups were identified. 16S rRNA gene sequencing also recovered several crenarchaeotal groups potentially involved in the initial anaerobic digestion processes. Overall, the two genes produced different taxonomic profiles for the digesters, while greater methanogen richness was detected using the mcrA gene, supporting the use of this functional gene as a complement to the 16S rRNA gene to better assess methanogen diversity. A significant positive correlation was detected between methane production and the abundance of mcrA transcripts in digesters treating sludge and wastewater samples, supporting the mcrA gene as a biomarker for methane yield
Neutral luminescent bis(bipyridyl) osmium(II) complexes with improved phosphorescent properties
No full textAn exploratory study on the antecedents of occupational commitment among hospitality educators in Hong Kong
No full textIn recent years, the growth in hospitality management programmes and the demand for hospitality educators in Hong Kong has increased dramatically. The hospitality industry, educational institutions and hospitality students also have high expectations of hospitality educators. Due to an increased need to recruit and retain qualified hospitality educators, this research seeks to understand the occupational commitment of hospitality educators in Hong Kong. Occupational commitment is an important indicator of a number of factors. It is found that occupational commitment is closely related to work-related stress, job satisfaction and occupational retention (Jamal, 1990; Borg et al., 1991; Jepson and Forrest, 2006; Klassen and Chiu, 2010). However, there is lack of research investigating the occupational commitment or its antecedents of hospitality educators. Therefore the study aims to gain an insight into the occupational commitment of hospitality educators in Hong Kong. This research identifies the key antecedents affecting occupational commitment and investigates which of these antecedents influence the occupational commitment of hospitality educators in Hong Kong. It also explores the reasons why hospitality educators are committed to hospitality education profession in Hong Kong. The research presented in this paper is one element of wider study. For this part, a qualitative research method and purposive sampling is adopted. Four focus groups comprised of twenty hospitality educators currently working in Hong Kong have been conducted. The results found that the flexibility of hospitality education work, non-shift work pattern, the self-actualization needs in education, career opportunities and occupational image are the key antecedents of occupational commitment of hospitality educators in Hong Kong
Effects of high-intensity intermittent running exercise in overweight children
No full textThis study examined the effects of a 6-week intermittent exercise training, at different intensities, on body composition, functional walking and aerobic endurance in overweight children. Forty-eight overweight children (age: 10.4 ± 0.9 years) were randomly assigned to either intervention or control group. Lower and higher intensity intermittent exercise groups (LIIE and HIIE) performed intermittent running three times a week. LIIE performed more intervals at a lower intensity [16 intervals at 100% of individual maximal aerobic speed (MAS), 8 minutes in total], and HIIE performed fewer intervals at a higher intensity (12 intervals at 120% of MAS, 6 minutes in total). Each interval consisted of a 15-second run at the required speed, followed by a 15-second passive recovery. After 6 weeks, HIIE had a significantly (p \u3c 0.05) higher percentage reduction in sum of skinfolds (i.e. calf and triceps), and significantly (p \u3c 0.05) fewer steps during the functional obstacle performance, as compared with LIIE and control group. Significant improvement (p \u3c 0.05) was found in intermittent aerobic endurance for HIIE as compared to the control group. Higher intensity intermittent training is an effective and time-efficient intervention for improving body composition, functional walking and aerobic endurance in overweight children
Baicalein exhibits inhibitory effect on the energy-dependent efflux pump activity in non-albicans Candida fungi
No full textUrbanization effects on spatial-temporal differentiation of tree communities in high-density residential areas
No full textThe changing mode of urban development through time can bring a varied landscape mosaic accompanied by spatial-temporal differentiation of urban vegetation. Hong Kong as an ultra-compact city generates intense interactions between trees and urban fabric to highlight urbanization effects on tree communities. The study areas cover public housing estates which accommodate about half of the 7.26 million population. Thirteen site factors related to estate, landform and habitat traits were measured or computed as surrogate urbanization effects. Species composition and diversity of tree communities in 102 estates were assessed by field surveys, including four estate groups: older or newer ones situated respectively in urban core or new towns. They contain 48,823 trees belonging to 232 species with heavy exotic representation. Total tree density and native tree density in newer estates were significantly higher than older ones. Differences in species richness and diversity and native species richness between older and newer estates were not significant, expressing to a certain extent the floristic-homogenization phenomenon. Multi-response Permutation Procedures (MRPP) results showed significant difference in species composition between older and newer estates, which could be explained by variations in development age, density, town plan and pre-urbanization land cover. Nonmetric Multidimensional Scaling (NMDS) results found tree distribution patterns in four estate groups strongly associated with estate area, open space area, estate population, estate age, Shannon index (H′) at planting strip and tree pit, and tree site quality index. Urban-forest management could be improved by adopting more native species and providing high-quality and spacious planting sites to accommodate more and larger trees. The research methods and findings could be used by policy makers and planners in similar large and developing cities to evaluate, design, maintain and enhance urban biodiversity
Childhood asthma and spirometric indices are associated with polymorphic markers of two vitamin D 25-hydroxylase genes
No full textBackground: Polymorphic markers of vitamin D pathway genes have been associated with asthma traits in different White populations. This study investigated the relationship between asthma phenotypes and single nucleotide polymorphisms (SNPs) of vitamin D receptor (VDR), vitamin D binding protein (GC), two 25-hydroxylases (CYP2R1 and CYP27A1), and 1α-hydroxylase (CYP27B1) in Hong Kong Chinese children.
Methods: 23 SNPs of the five vitamin D pathway genes were successfully genotyped in 914 asthmatic children and 1231 non-allergic controls. Genotypic and haplotypic associations with asthma phenotypes (diagnosis, spirometric indices, total IgE, and eosinophil percentage) were analyzed by multivariate regression. Generalized multifactor dimensionality reduction was used to detect epistatic interactions between SNPs for asthma phenotypes.
Results: Several SNPs of CYP27A1, CYP27B1, GC, and CYP2R1 were associated with asthma or spirometric indices, although only the association between FEV1 and CYP2R1 rs7935792 passed Bonferroni correction (p = 2.73 × 10(-4) ). Patients with CC genotype of rs7935792 had higher FEV1 than those with the other two genotypes. Asthma was also associated with TT haplotype of CYP27A1 and AGGATA haplotype of CYP2R1 (p = 0.021 and 0.024, respectively). Besides, strong association was found between FEV1 and GATAG of CYP2R1 (β = 13.37, p = 4.83 × 10(-4) ). GMDR failed to identify any 2-locus to 4-locus interaction that modulated asthma or spirometric indices.
Conclusions: Several SNPs and haplotypes of CYP2R1 are associated with asthma diagnosis and FEV1 in children. Asthma is also modestly associated with a CYP27A1 haplotype. These two 25-hydroxylase genes may be genetic determinants for asthma phenotypes in children