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How CXCR4 Knockdown Affects Tumorigenic Properties in ES8 Cells
No full textC-X-C chemokine receptor type 4 (CXCR4) is a seven-transmembrane G-protein–coupled receptor (GPCR). When activated by its ligand SDF-1 (CXCL12), CXCR4 triggers signaling pathways that promote cell survival, proliferation, angiogenesis, and chemotaxis. Many cancers exploit the CXCR4/SDF-1 axis to support tumor growth and metastasis. Ewing sarcoma (ES8) is an aggressive pediatric bone and soft-tissue cancer, and elevated CXCR4 signaling has been linked to increased migration and metastatic behavior. ES8 cells, a well-established ES8 cell line, provided a model to study how CXCR4contributes to tumor progression. CRISPR-Cas9 genome editing was used to knock down CXCR4 in ES8 cells, delivered through lipid-based transfection. Lipofection allows Cas9 and guide RNA to enter the cell and introduce targeted gene disruption. This project evaluated how CXCR4 loss affects tumorigenic properties, including proliferation, colony formation, migration, and survival. We hypothesized that reducing CXCR4 expression would decrease these behaviors, given its established role in promoting aggressive and metastatic phenotypes in Ewing sarcoma.https://commons.stmarytx.edu/mecofdis/1001/thumbnail.jp
Syntax and Grammar
No full textSentence examples written in Ietic and translated to English. Descriptions of how transformations, nouns, conjunctions and verbs are used in our language.https://commons.stmarytx.edu/docsinvlang2501/1004/thumbnail.jp
Theshrahk Thwath Legend
No full textThis document contains the origin myth of Theshrahk Thwath’s vegetarianism and relationship with nature. It is presented line-by-line in Theshalshe, the orthography, the romanized form, a transliteration, and an English translation.https://commons.stmarytx.edu/docsinvlang2502/1004/thumbnail.jp
Muzibogun the Bells of St. Mary’s
No full textThis is a portion of our school song translated to Muzibogunhttps://commons.stmarytx.edu/docsinvlang2503/1000/thumbnail.jp
