Indonesian Journal of Obstetrics and Gynecology (INAJOG)
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p53 Gene Codon 72 Polymorphisms among Cervical Carcinoma Patients
Get PDFObjective: To identify the association between p53 gene codon 72
polymorphism and cervical carcinoma.
Method: An analytic observational study with case-control design,
from November 2013 until March 2014 in the Department of Obstetrics
and Gynecology and Microbiology Laboratory Faculty of Medicine,
Sriwijaya University, Dr. Moh. Hoesin Hospital Palembang.
Result: In total there are 100 samples analyzed consisting of 50 subjects
and 50 control groups. Genotype distribution in subject group
are 54% Arg/Arg, 42% Pro/Arg and 4% Pro/Pro, and in control
group are 36% Arg/Arg, 46% Pro/Arg and 18% Pro/Pro. Arg/Arg
genotype is at risk of cervical carcinoma 6.7 times higher compared
with Pro/Pro genotype (p=0.013; OR 6.75; 95% CI 1.34-34.94). Arg
allele in the p53 gene codon 72 increase the risk of cervical carcinoma
2.6 times more than Pro allele.
Conclusion: Proline mutation to Arginine in gene p53 P72R is one of
the risk factor for cervical carcinoma.
Keywords: arginine, cervical carcinoma, gene p53 codon 72, polymorphism,
prolin
Pereira Suture: an Alternative Compression Suture to Treat Uterine Atony
Get PDFObjective: To evaluate the use of Pereira suture as a conservative surgical
management in postpartum hemorrhage due to uterine atony.
Methods: The study was performed in the Department of Obstetrics
and Gynecology, Temanggung General Hospital, Central Java, Indonesia.
Data was retrieved retrospectively from medical records,
from January 2011 until December 2013.
Results: Pereira suture was done in four cases of uterine atony with
failed conservative management, and the procedure was found to be
successful in all cases.
Conclusion: Pereira suture is an alternative surgical procedure for the
treatment of uterine atony after failed conservative management.
[Indones J Obstet Gynecol 2015; 3: 177-182]
Keywords: pereira suture, postpartum hemorrhage, uterine aton
Effectiveness of Pelvic Organ Prolapse Surgery in Women with Depressive Symptoms and Decreased Quality of Life
Get PDFObjective: To know the effectiveness of pelvic organ prolapse surgery
in decreasing depressive symptoms (based on PHQ9) and improving
quality of life in women with pelvic organ prolapse.
Methods: This experimental study without control is conducted at
the Obstetrics and Gynecology Department of Dr. Mohammad
Hoesin Hospital Palembang/Faculty of Medicine Sriwijaya University,
from October 03 2012 until May 31, 2014. Data containing selfadministrated
questionnaire about depressive symptoms (PHQ9)
and quality of life (PFIQ and PFDI) were recorded. Questionnaire
was performed before and six months after surgery. Sample included
26 women with pelvic organ prolapse seeking pelvic organ
prolapse surgery, which qualified the inclusion criteria. Data were
analyzed using Chi Square and Fisher Exact test. Data analysis was
done using SPSS 18.0.
Results: According to paired T test there is a significant difference
between mean PHQ9 score before (6.69±3.80) and 6 months after
surgery (1.96±1.75)(p=0.001). Total PFIQ score decreased from
17.15±9.39 to 2.88±4.01 with 14.27±5.38 reduction. PFDI score before
surgery were 29.85±15.73 and decreased to 11.50±10.99, with
a reduction of 18.35±4.74.
Conclusion: There was significant reduction in depressive symptoms
and improved quality of life in women with prolapse after surgery,
compared to before surgery.
[Indones J Obstet Gynecol 2015; 3-4: 196-199]
Keywords: depression, quality of life, uterine prolaps
PPARy Expression in Eutopic and Ectopic Endometrium of Reproductive Age Women with Endometriosis
Get PDFObjective: To evaluate the expression of PPARy receptor and to
compare its expression in eutopic and ectopic endometrium in
women with endometriosis.
Method: This is a cross sectional study. Ten female subjects with endometriosis
that underwent laparoscopy or laparotomy who fulfilled
the inclusion criteria were recruited by consecutive sampling.
Two samples were taken, eutopic endometrium and ectopic endometrium
from endometriosis cyst wall during surgery of each
subject. PPARy expression was examined by two-step RT-qPCR. Our
data was statistically examined using the paired t-test and Pearson’s
correlation test.
Result: PPARy was found to be expressed in eutopic and ectopic endometrium
of women with endometriosis using the RT-qPCR method.
The expression of PPARy was not statistically different in
eutopic and ectopic endometrium (1.16 relative fold vs 1.25 relative
fold; p=0.26). By Pearson’s correlation there was a weak positive
correlation between PPARy expression of eutopic and ectopic endometrium
(r=0.16).
Conclusion: PPARy was detected by two-step RT-qPCR in eutopic
and ectopic endometrium of women with endometriosis. Semiquantification
of PPARy expression showed that there was no significant
difference between PPARy expression in eutopic and ectopic endometrium
of women with endometriosis. There was a weak positive
correlation between PPARy expression in eutopic and ectopic endometrium
of women with endometriosis.
[Indones J Obstet Gynecol 2015; 3-4: 200-205]
Keywords: endometriosis, PPARy, two-step RT-qPC
Effect of Smoking on Advanced Stage Cervical Cancer Patient Survival
Get PDFObjective: The researchers aim to investigate the relationship between
smoking habit and other factors as prognostic factors of cervical
cancer.
Method: We performed a retrospective and prospective cohort study
with subjects that are stage IIB-IVB cervical cancer patients in Dr.
Cipto Mangunkusumo Hospital followed up from August 2009 to
April 2014. The subjects’medical records were reviewed, and patients
were interviewed about their current condition by telephone. Questions
asked include smoking habit, spouse’s smoking habit, and mortality
status. Patients that could not be contacted by phone were excluded
from the study. Statistical analysis was done using Stata 10.
Result: Out of 390 cervical cancer patients stage IIB-IVB in 2009,
there were 270 patients (69.2%) that were included in the inclusion
criteria. Most of the patients are 40-59 years old (82.2%) and are nonsmokers
(91.8%). The most frequent clinicopathological characteristic
is IIIB (63.3%) and squamous cell carcinoma (71.9%). The 5-
year survival rate is 22.6%. There is no statistical significance between
advanced stage cervical cancer survival with the patients’ or patients’
husbands’ smoking habit.
Conclusion: In our study, smoking habits do not aggravate survival
rate of advanced stage cervical cancer patients but further research
must be done with more sample. Stage, and tumor size both by physical
examination and ultrasound can be used as the prognostic factor.
[Indones J Obstet Gynecol 2015; 3: 170-176]
Keywords: Brigmann Index, cervical cancer, smoking, surviva
Factors Affecting Selection of Contraceptive Methods and Its Length of Use
Get PDFObjective: To evaluate the factors related to the selection of contraceptive
methods and length of use on acceptors in Raden Saleh Clinic
and Fatmawati General Hospital.
Method: The study was a prospective observational study designs.
The factors that we observed include the family planning factor (wife
age, number of desired children and infertility), subjective factors
(side effect experience of contraception, support from the husband/
family, and religion), objective factors (medical disorders, person
helping to select contraception, family planning service centre
and availability of contraceptives) and the level of motivation (level
of education). All the clients who will receive contraception and meet
the criteria for the research were interviewed and given questionnaires
until the desired sample size is achieved. The study was conducted
at the Raden Saleh Clinic and Fatmawati General Hospital. We
then conducted follow-up at 3 and 6 months after the use of the contraceptive
methods to assess the length of use.
Result: The total number of subjects was 151 people. The average
age of respondents was 31 years old, with the contraception options
being IUD (67.1%), implants (17.8%), sterilization (7.2%), injectable
contraception (3.9%), and oral contraception (3.9%). From all
the factors studied, only the number of desired children were found
to affect the selection of contraceptive methods significantly
(p=0.008) in Fatmawati General Hospital, while in Raden Saleh Clinic
all of the factors did not affect the selection of contraceptive method
(p>0.05). We also found that all of these factors do not have a significant
relationship to the length of contraceptive use (p>0.05). There
were 6.6% of subjects (n=10) who switched the type of contraception.
From all of them, there was a trend of switching from oral contraceptive
to injectable method (33.3%) and from IUD to injectable
method (66.7%).
Conclusion: Only the number of desired children has an effect on the
selection of contraceptive methods in Fatmawati General Hospital,
while in Raden Saleh Clinic all of the factors studied do not affect in
selection of contraceptive methods. Family planning factors, subjective
factors, objective factors and motivation levels have no effect on
the length of contraceptive use by clients at Raden Saleh Clinic and
Fatmawati General Hospital.
Keywords: contraception methods, factors, length of use, selectio
Maternal Serum Interleukin6 Level in Correlation with Preterm Delivery
Get PDFObjective: To determine the correlation between maternal serum
Interleukin-6 (IL-6) serum level with preterm delivery.
Methods: We performed an observational cross-sectional study of 30 pregnant women between 21 to 36 week gestational age with and wthout labour (delivery) in Prof. Dr. RD. Kandou Hospital Manado. Samples were collected with consecutive sampling method. Data was analyzed using Mann-Whitney test with significance level of
BRCA1 Gene Q356R (1186A=G) Polymorphism and Epithelial Ovarian Cancer Incidence
Get PDFObjective: To determine the association between BRCA1 gene
Q356R (1186A=G) polymorphism and epithelial ovarian cancer incidence.
Methods: This study is an observational analytic study with casecontrol
study design. All patients diagnosed with epithelial ovarian
cancer that were treated in the outpatient clinic and inpatient ward
of the Department of Obstetrics and Gynecology, Dr. Mohammad
Hoesin Hospital, Palembang who met the inclusion criteria were included
in this study. DNA extraction was performed on blood samples,
followed by PCR-RFLP process.
Results: We obtained the genotype distribution of BRCA1 Q356R
(1186A=G) polymorphisms to be QQ genotype (wild-type) on all 50
subjects in the case group (100%) and 50 control subjects (100%).
Similarly, all BRCA1 alleles have the Q allele. The results of this study
found no polymorphism of the BRCA1 Q356R (1186A=G) in the
ovarian cancer and control groups.
Conclusion: Polymorphism of BRCA1 gene Q356R (1186A=G) was
not significantly associated with epithelial ovarian cancer incidence.
[Indones J Obstet Gynecol 2015; 3-4: 206-211]
Keywords: BRCA1 gene, epithelial ovarian cancer, polymorphis
Fascin Expression as Prognostic Factor for Survival in Advanced Epithelial Ovarian Carcinoma
Get PDFObjective: To evaluate fascin expression as a prognostic factor and
its correlation with survival and clinicopathologic factors (degree of
differentiation and stage) in epithelial ovarian carcinoma.
Methods: This study is prognostic study with historical cohort design.
Fascin was analyzed in paraffin block sections of 33 advanced
stage ovarian carcinoma patients using immunohistochemistry. Fascin
expression was tested for its correlation with overall survival as
well as with grade and stage of the cancer.
Results: In this study, fascin expression has no correlation with survival.
In the period of 17-22 months, samples with high fascin expression
had a HR of 1.59 (95% CI=0.38-6.67, p=0.449), but in the
period of 17-23 months, both groups had comparable HR. In the period
of more than 23 months, samples with high expression of fascin
had a better HR of 0.40 (95% CI=0.04-4.38, p=0.449). No significant
correlation was found between fascin expression with grade
(OR=2.08, 95% CI=0.44-9.84, p=0.442) and stage (OR=2.70, 95%
CI=0.39-18.96, p=0.360).
Conclusion: In this study, there was no correlation between fascin
expression and survival, and also no correlation between fascin,
grade and stage. Further study with a larger, more homogenous
sample, analyzing confounding factors is needed.
[Indones J Obstet Gynecol 2015; 3-4: 222-229]
Keywords: advanced stage ovarian carcinoma, fascin, surviva