Bioscience Journal
Not a member yet
3143 research outputs found
Sort by
Synthesis and evaluation of toxicity and antimicrobial activity of rifampicin associated with iron oxide nanoparticles
Full text linkRifampicin has broad-spectrum antimicrobial activity, but it can cause nephrotoxic and hepatotoxic damage because high doses are required. Nanosystems emerge as a perspective to improve the transport systems of this drug. In this work, iron oxide nanoparticles were synthesised, functionalized with lauric acid, and rifampicin was incorporated into the nanosystem. The samples were characterized by spectroscopic techniques: electronics in the visible ultraviolet region (UV-vis), vibrational absorption in the infrared region (IR), X-ray diffractometry (XRD), and dynamic light scattering (DSL). The toxicity of the nanocompounds and the antimicrobial activity against Staphylococcus aureus ATCC 25923 were studied by the Artemia salina lethality and disc diffusion techniques, respectively. As a result, IR analysis showed characteristic vibrations of laurate and rifampicin on the surface of the nanosystem. The presence of magnetic iron oxide was confirmed by XRD and the mean diameter of the crystallites was 8.37 nm. The hydrodynamic diameter of rifampicin associated with the nanosystem was 402 nm and that of the nanosystem without rifampicin was 57 nm. The compounds did not show toxicity to Artemia salina and the in vitro antimicrobial activity against Staphylococcus aureus was slightly decreased when rifampicin was associated with the nanosystem. In general terms, the results showed that iron oxide nanoparticles showed no toxicity and reduced the toxicity of rifampicin by 41.54% when carried compared to free rifampicin. Therefore, magnetic iron oxide nanoparticles may have the potential to act as a platform for associated drugs
Complications after percutaneous transluminal coronary angioplasty and associated factors
Full text linkThis study aimed to analyze the incidence of vascular complications and associated factors in patients undergoing elective percutaneous transluminal coronary angioplasty. This study is observational, quantitative, and longitudinal, and followed 50 patients undergoing elective percutaneous transluminal coronary angioplasty. An instrument for the sociodemographic, clinical, procedure, and vascular complications characterization was used for data collection. And descriptive statistics, bivariate analysis, and multiple binomial logistic regression were used for data analysis. The level of statistical significance considered was 95%. It was detected the prevalence of male patients (70%), elderly (54%), and diagnosed with systemic arterial hypertension (72%). As for the percutaneous access route prevailed the radial approach (64%). Age and body mass index were identified as possible risk factors for vascular complications. In the 50 procedures performed, there was a prevalence of hematomas (20%) and bleeding (10%). Among the complications prevailed radial Early Discharge After Transradial Stenting of Coronary (60%), large femoral hematoma (20%), small femoral hematoma (20%), and bleeding (Bleeding Academic Research Consortium 2) (100%). The results concluded an elevated incidence of vascular complications in the first 24 hours after elective percutaneous transluminal coronary angioplasty. This study contributes to research, assistance, and training in health and nursing by identifying post-PTCA vascular complications, minimizing their progression, handling their management, and developing health care safety protocols
Agronomic characteristics and optimized sweet potato root production in monoculture under green manuring
Full text linkOne of the challenges of the scientific research on sweet potatoes in semi-arid environments is to increase biomass amounts of spontaneous species from the Caatinga biome, such as hairy woodrose (Merremia aegyptia L.) and roostertree (Calotropis procera Ait.), for use as green fertilizers during cultivation. Therefore, this study aimed to agronomically and economically optimize the agronomic characteristics of sweet potato root production in a monoculture, fertilized with equal amounts of biomass mixture of these spontaneous species, over two years of cultivation. The experimental design was complete randomized blocks with five treatments and five replications. The treatments consisted of equal amounts of hairy woodrose and roostertree biomass at 16, 29, 42, 55, and 68 t ha-1 on a dry basis. An additional sweet potato treatment was planted in each experiment, one without fertilizers (control) and another with mineral fertilizer, to compare with the treatment of maximum physical or economic efficiency. Sweet potato fertilization obtained the maximum optimized productive efficiency by incorporating 46.97 t ha-1 of dry biomass of M. aegyptia and C. procera into the soil. The maximum optimized agroeconomic efficiency (based on net income) of sweet potato cultivation occurred by adding 41.55 t ha-1 of dry biomass of M. aegyptia and C. procera to the soil. Using biomass from the green fertilizers M. aegyptia and C. procera is a viable technology for producers who practice sweet potato monocropping in semi-arid environments
Relationships between body mass index, ultrasound measurements and, internal body fat depots in pelibuey sheep
Full text linkThe objective of this study was to evaluate the relationship between body mass index (BMI), ultrasound measurements (USM), and internal fat (IF) in hair sheep. The BMI and USM were determined 24 hours before laughter in 35 animals finished in intensive fattening. At slaughter, the IF (inner adipose tissue) was dissected, weighed, and grouped as mesenteric, omental, or perirenal fat; these three depots were summed to obtain their total weight. The relationship of the variables was evaluated through Pearson\u27s correlation coefficient (r) using the CORR procedure and by regression models using the REG procedure of SAS ver. 9.3. The IF and BMI presented r= 0.75 (P <.0001), while the IF and USM presented an r that varied from 0.70 to 0.80 (P <.0001). Among the internal fat deposits, the perirenal was the one that presented minor correlation coefficients with BMI (r = 0.55), subcutaneous fat thickness (0.55), and thoracic Longissimus dorsi area) (r = 0.33), and for this, the last correlation was not significant (P>0.05). The regression equation between IF and BMI had a coefficient of determination (r2) of 0.67 (P <.0001). The inclusion of the subcutaneous fat thickness together with the BMI improved the r2 with a range of 0.77. The BMI and the subcutaneous fat thickness could be used to predict the weight of the internal fat in hair sheep
What is the optimal fertigation start time and frequency in lettuce seedlings?
Full text linkAlthough lettuce is one of the most important vegetable crops cultivated in Brazil, producers conduct seedling production empirically, as there are no published reports on the optimal start time and management strategy for seedling fertigation. The present aimed to assess the influence of fertigation management on the growth, physiological aspects and nutritional status of lettuce seedlings and to determine the optimal fertigation start time and frequency. Two experiments were conducted, each with a randomized block design and six repetitions. The first consisted of six treatments, namely six fertigation start times at 0, 3, 6, 9, 12, and 15 d after emergence (DAE), and the second consisted of five treatments, representing different application frequencies at 3, 4, 5, 6, and 7 d intervals. The assessment of nutrient accumulation levels and biometric and physiological characteristics of the seedlings were performed after transplanting. Fertigation start times significantly affected 14 of the 18 variables assessed, particularly the number of leaves, shoot dry weight, leaf area, initial chlorophyll fluorescence, and P, K, Ca, Mg, and S accumulation. The best results for ten variables were obtained when fertigation began at emergence, with values 17.77 - 35.63% higher than those at fertigation onset at 15 DAE. Application frequency only influenced chlorophyll content and N, P, K, and S accumulation, with optimal results obtained at 3 - 6 d intervals. Beginning fertigation at plant emergence favors dry weight production, nutrition and photosynthesis and shortens the production time of lettuce seedlings. The optimal start time for lettuce seedling fertigation is at emergence, with application performed every 6 d
QUALIDADE DO LEITE CRU REFRIGERADO PRODUZIDO NO NORDESTE DO BRASIL
No full textThe objective of this study was to evaluate the seasonal influence on the chemical composition, somatic cell count (SCC), and total bacterial count (TBC) of raw bulk-tank milk in northeastern Brazilian states. Data were obtained from milk samples from tanks collected monthly by industries registered with the Federal Inspection Service. According to normative instruction #62 (IN-62), two validity periods were considered. The highest recorded averages for chemical composition were between May and July. The mean fat content varied from 3.51% to 3.69%, and the protein content ranged from 3.07% to 3.17%. The averages of SCC ranged from 4.66 to 4.90 × 1,000 cells/ml, with the highest being recorded in July. At the same time, the TBC ranged from 2.34 to 2.53 cfu/ml. The highest TBC was recorded in March. The mean values of fat, protein, defatted dry extract, SCCs, and TBC were influenced by the months of the year. The means for these variables decreased in periods when Brazilian legislation was more severe. However, the SCC and TBC averages found in this study were still high, considering the quality of raw milk production. SCC and TBC presence still did not comply with the limits established by the legislation.O objetivo deste estudo foi avaliar a influência sazonal na composição química, contagem de células somáticas (CCS) e contagem bacteriana total (CBT) do leite cru de tanques a granel em estados do Nordeste brasileiro. Os dados foram obtidos em amostras de leite de tanques coletados mensalmente pelas indústrias cadastradas no serviço de inspeção federal. Foram considerados dois períodos (1 e 2) de validade de acordo com a Instrução Normativa-62 (IN-62). As maiores médias registradas para composição química, foram entre maio e julho. O teor médio de gordura variou de 3,51 a 3,69% e o teor de proteína de 3,07 a 3,17%. As médias do escore de células somáticas variaram de 4,66 a 4,90 mil células/ml com a maior sendo registrada em julho, enquanto a contagem bacteriana transformada variou de 2,34 a 2,53 ufc/ml e a maior foi registrada no mês de março. Os valores médios de gordura, proteína, extrato seco desengordurado, contagens de células somáticas e contagens bacterianas totais foram influenciados pelos meses do ano e tiveram suas médias decrescentes para períodos mais rígidos da legislação brasileira; entretanto, as médias de CCS e CBT encontradas neste estudo ainda são elevadas considerando a produção de leite cru de qualidade e ainda apresentam não atendimento aos limites estabelecidos pela legislação
Application of limestone-molasses blends in Nile tilapia rearing tanks
Full text linkThe work aimed at determining the effects of applying different blends between liquid molasses and agricultural limestone on water quality and growth performance of Nile tilapia juveniles, Oreochromis niloticus. The study employed 24 indoor tanks of 100 L and 24 outdoor tanks of 250 L. Four control groups were set up (no product applied, only molasses, only limestone, molasses-limestone blend with 48% limestone but no fish) and two experimental groups (molasses-limestone blends with 32% and 48% molasses), with four replicates each. Over 11 weeks, the water quality was monitored systematically. The 24-h cycle monitoring of temperature, pH, specific conductance, dissolved O2 and total alkalinity were carried out at the 8th week. The blends between molasses and limestone accelerated the dissolution rate of the agricultural limestone in water. The blend containing 48% molasses led to greater increases in water alkalinity and pH in relation to tanks with only limestone applications. However, the molasses-limestone blend applications impaired the Nile tilapia growth performance, especially in the outdoor tanks. While the fish yield was 25.1 g m-3 day-1 in the molasses outdoor tanks, it was equal to 22.8 g m-3 day-1 in the molasses (32%)-limestone units (P<0.05). It has been concluded that the blending between limestone and molasses brings no clear benefits to Nile tilapia’s rearing tanks when compared to the limestone-only tanks.O trabalho teve por objetivo determinar os efeitos da aplicação de diferentes misturas entre o melaço líquido e o calcário agrícola, na qualidade da água e no desempenho zootécnico de juvenis de tilápia do Nilo, Oreochromis niloticus. O estudo utilizou 24 tanques de 100 L, em sistema de cultivo indoor, e 24 tanques de 250 L, em sistema de cultivo outdoor. Foram constituídos quatro grupos-controle (nenhum produto aplicado na água, somente melaço, somente calcário, mistura melaço-calcário com 48% de melaço, mas aplicada em tanques sem peixes) e dois grupos-experimentais (mistura melaço-calcário com 32% e 48% de melaço), com quatro repetições cada. Ao longo de onze semanas, realizou-se o monitoramento da qualidade da água dos tanques. O monitoramento nictimeral da temperatura, pH, condutância específica e das concentrações de O2 e alcalinidade total da água foi realizado ao final da 8ª semana. A mistura melaço-calcário, contendo 48% de melaço, acelerou a taxa de dissolução do calcário na água, levando a um maior aumento na alcalinidade e pH, em relação aos tanques que receberam somente o calcário. Apesar disso, a aplicação da mistura melaço-calcário prejudicou o desempenho zootécnico dos juvenis de tilápia, principalmente nos tanques outdoor. Concluiu-se que práticas de calagem que levem a mais rápidas elevações na alcalinidade da água não são apropriadas para tanques de criação da tilápia do Nilo
Epidemiological analysis of Paramphistomum species in Ruminants in selected Districts of Khyber Pakhtunkhwa (KPK), Pakistan
Full text linkThis epidemiological study was conducted to compare the climatic conditions of four different districts of Khyber Pakhtunkhwa, Pakistan, in terms of the prevalence of parasitic infection Paramphistomum in cows and buffaloes. A total of 2400/cows and buffaloes (200/per month and 50/per district) fecal samples were collected. The samples were examined for Paramphistomum egg contamination by direct microscopic examination and sedimentation methods. The infectivity ratio was 17.5% in cows and 17% in buffaloes; the higher rate of infection was recorded in autumn with 41% in cows and 36% in buffaloes. Whereas the lowest ratio was 6.5% in cows and 7% in buffaloes recorded during spring. It was also noted that a high rate of prevalence was recorded in older animals (higher than 2 years of age) than younger (less than 2 years of age), while gender-wise, it was observed that male animals were more affected than females. Prevalence was higher in cows in Mardan district (19.30%) followed by Nowshera (19.10%) and Swabi (17.80%) districts while lowest in Charsadda district (13.80%) where in buffaloes the higher prevalence was recorded in Swabi (18.80%) followed by Mardan (18.60%) then Charsadda (16.80%) while lowest in Nowshera (13.80%)
Bioclimatic zoning for quails in the rain season in the state of Paraíba, Brazil
Full text linkThis work aimed to perform bioclimatic zoning based on the temperature and humidity index (THI) for quails from the 3rd to the 5th week of life during the rainy season in the state of Paraíba, Brazil, in order to identify the most suitable mesoregions among the Zona da Mata, Agreste, Borborema and Sertão for the breeding of these birds and to propose mitigating measures for the thermal comfort of the animals. The monthly air temperature and relative humidity data for calculating the THI were obtained from conventional meteorological stations of the National Meteorological Institute of the Brazilian federal government from 1961 to 2015, in turn making maps with the spatial distribution of the index. The index ranged from 71 to 76 in the various mesoregions of Paraíba, with Agreste being the best mesoregion for breeding quails from the 3rd to the 5th week of life, thus providing a thermal comfort zone for birds and only requiring a few corrective measures in their facilities, when necessary, followed by the Sertão, Zona da Mata and Borborema mesoregions
Poultry litter and cover crops influence the agronomic performance and grain yield of wheat
Full text linkThe use of poultry litter as a source of nitrogen (N) and the decomposition of ground cover plants can be an alternative and N management strategy in the wheat crop, in succession. Therefore, the objective of this study was to evaluate the effect of isolated and/or intercropped cultivation of ground cover plants in autumn/winter and the use of poultry litter on some plant parameters and on the final yield of wheat grains. The experimental design was randomized blocks, in split plots, with three replications. The treatments in the plots were composed of isolated coverings Avena strigosa Schreb., Raphanus sativus L. and intercropping of A. strigosa + R. sativus and fallow as a control. In the subplots, the N managements in the wheat crop with 100% of the N recommendation via poultry litter, 100% of the N via mineral (urea), 50% via poultry litter + 50% mineral and the control, without N application. The management of nitrogen fertilization and the cover crops altered the yield components of the wheat crop. The use of poultry litter increased the final grain yield, being an alternative as a partial replacement, when associated with urea, or total N. Isolated crops R. sativus and A. strigosa + R. sativus intercropping significantly influenced agronomic performance and final grain yield