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Expecting a child conceived by medically assisted reproduction in the context of infertility: A qualitative case study of the experience of pregnant women and their partners
No full textBackground: Infertility affects around one in six people worldwide. Technological advances and changes in legislation in medically assisted reproduction (MAR) are helping more people achieve parenthood after a journey marked by infertility. Yet the unique experience of women who become pregnant after MAR, and of their partners, remains little known. Aim: To describe the experience of pregnant women and their partners during a pregnancy resulting from infertility-related MAR by examining their emotions throughout the pregnancy, their parental identity construction, and transformations experienced in various spheres of their life. Methods: We conducted an integrated case study using a descriptive qualitative approach. Semi-structured interviews were conducted with 21 participants from Quebec (Canada), including 13 pregnant women and eight partners (five men, three women) who had conceived by infertility-related MAR. The data were analysed abductively. Findings: The pregnant women and their partners experienced intense emotions throughout the pregnancy, oscillating between joy, fear, and relief. Parental identity construction was marked by expectation, hope, and a transition centred on pregnancy and infertility. They also transformed their relationships, adapting as a couple, redefining family and social ties, and entering a new normal with healthcare professionals. Conclusion: For pregnant women and their partners, pregnancy after infertility-related MAR is fraught with paradoxical emotions and marked by challenging parental identity construction. Social and family recognition of their journey influences how they navigate the transitional period of pregnancy and project themselves into their parental role. Personalised, empathetic support from healthcare professionals is essential to support them during pregnancy and facilitate their transition to parenthood
Converging evidence of a specific vulnerability of young boys to parental childhood trauma
No full textAbstract
Objective
Childhood trauma is common among parents and can have intergenerational effects. Preliminary evidence suggests that young boys may be more vulnerable to maternal childhood trauma than girls. This finding needs to be replicated, and it remains to be determined whether it also applies to paternal childhood trauma. The current study aims to examine the associations between parental childhood trauma and 3 indicators of early functioning (general development, socioemotional development, and temperament) in offspring and to assess the moderating role of child sex.
Method
Childhood trauma was assessed during pregnancy in 3 community samples of women (N = 143; N = 195; N = 216) and postnatally in 1 community sample of fathers (N = 165). Child functioning was assessed using parental reports of child development and temperament between 6 and 38 months postnatal. Regression-based moderation analyses were conducted.
Results
Significant associations between parental childhood trauma and adverse child outcomes were observed in all samples. A moderating role of child sex was supported in 3 of the 4 samples, all indicating greater vulnerability to parental childhood trauma among boys.
Conclusion
These findings suggest that maternal and paternal childhood trauma are associated with poorer functioning in infants and toddlers, but only in boys. This has implications for personalized preventive interventions with parents exposed to childhood trauma. These results call for replication with large and diverse samples including biological measures and observational measures of child development
Les facteurs explicatifs du recours répété aux mesures contraignantes sous forme d’intervention disciplinaire en milieu scolaire
Full text linkLa seigneurie Massue : régime foncier et enrichissement capitaliste dans la région de Yamaska (1833-1854) à travers les archives notariales
Full text linkLe rôle médiateur des comportements parentaux dans l’association entre le stress parental et le développement de l’enfant à l’âge de 1 an
Full text linkUsing multilingual books in kindergarten classrooms with the ELODIL Multilingual Picture Books digital platform: Outcomes and critical perspectives
No full textIn this chapter, the creation of a digital platform featuring multilingual picturebooks and its implementation in ten kindergarten classrooms in a pluri-ethnic and multilingual environment, as part of an action-research project is first described. The aim of the project was to provide teachers with tools to encourage openness to linguistic diversity and support children’s language development. Secondly, the intervention is presented and its main benefits for children, their parents, and teachers are summarised. Thirdly, a critical examination of certain aspects of the research project and its digital platform is conducted to ensure a greater awareness of the issues surrounding the theoretical approach in which it is inscribed, the platform itself, and the context in which the platform is used. Shedding light on these issues is a necessary step toward improving the understanding and implementation of inclusive education principles. © 2025 selection and editorial matter, Esa Christine Hartmann and Áine McGillicuddy
Multifactorial interaction and influence of culture conditions on yellow fluorescent protein production in Phaeodactylum tricornutum
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Phaeodactylum tricornutum is a promising host for light-driven synthesis of heterologous proteins. However, the marine cold-water environment and alkaline-acidic pH shifts in the culture, necessitated by the diatom's growth requirements. In this study, we analyzed the influence of growth condition on maturation and dynamics of the yellow fluorescent protein (YFP) in episomal-transformant P. tricornutum. A mathematical model was developed to detect the parameters that affect biomass and YFP production. Optimized conditions increased YFP mean fluorescence intensity (MFI) per cell by 4.2-fold (3.6 ± 0.6 to 15.4 ± 1.1) and total protein levels in the culture by 1.8-fold (123 ± 4 to 219 ± 9 µg L−1), without affecting biomass. YFP stability studies in P. tricornutum showed that the ubiquitin–proteasome system contributes the degradation of the recombinant protein, whereas newly synthesized YFP remains stable for up to 12 h. This optimization provides insights into the fluorescent protein-based heterologous production in diatoms
Extrachromosomal expression of functional Cannabis sativa cannabidiolic acid synthase in Phaedodactylum tricornutum
Full text linkAbstract
Cannabis sativa's cannabidiolic acid (CBDA) offers significant therapeutic potential without inducing psychotropic effects but is typically found as part of a complex mixture of metabolites in plant extracts. Using a heterologous expression platform could allow the production of pure CBDA. Here, we propose to express CBDA synthase (CBDAS) in Phaeodactylum tricornutum. Episomes carrying CBDAS variants, incorporating the native signal peptide (CBDAS) or the highly abundant secreted protein 1 secretory signal peptide (SP:CBDAS) were constructed. CBDAS variants were tagged with the yellow fluorescent protein (YFP), introduced into the marine diatom, and screened by fluorescence. Confocal microscopy revealed that CBDAS and SP:CBDAS arranged in aggregated structures indicative of secretory pathway involvement. Western blot assays confirmed whole construct accumulation intracellularly, while soluble YFP was detected extracellularly. Finally, enzymatic assays showed CBDA production by both CBDAS and SP:CBDAS strains, confirming the potential of P. tricornutum as a platform for cannabinoid biosynthesis
