Publicatii USAMV Cluj-Napoca (University of Agricultural Sciences and Veterinary Medicine / Universitatea de Ştiinţe Agricole şi Medicină Veterinară din Cluj-Napoca)
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THE IMPORTANCE OF THE LONG-EARED OWL ASIO OTUS OTUS (L.) IN RODENT CONTROL
Get PDFThe trophic spectrum of the long-eared owl was studied in two localities of Someshul Mic basin: Cluj-Napoca city and Cojocna village. 2131 pellets were collected: 1403 pellets from Cluj-Napoca Botanical Garden and 728 from Cojocna. 6161 micromammal and bird skulls and bones were separated: 4238 from the pellets collected in Cluj-Napoca and 1923 from those collected in Cojocna. The rodents percent was 84,52% in Cluj-Napoca and 99,12% Cojocna pellets. 4 insectivore species, 12 rodent species and 10 bird species were found in pellets. The most abundant and the dominant species is the common vole (Microtus arvalis) with 64,08% in Cluj-Napoca and with 79,43% in Cojocna. Among birds the most abundant are the house sparrow and the tree sparrow, which constitute almost 70% from all the birds
EPIGALLOCATECHIN GALLATE ANTIOXIDANT EFFECT IN RETINAL PIGMENT EPITHELIAL CELLS
Get PDFCathechins are members of the large family of polyphenols and major compounds of tea leaves. Epigallocatechin-3-gallate accounts for more than 50 % of tea catechins. Their beneficial effect for human health is well documented from epidemiological, interventional and in vitro studies as well. Most of the health benefits of catechins are related to their antioxidant properties. Retina is a tissue with high oxygen consumption and subject to the aggression of reactive oxygen species (ROS). Retinal pigment epithelium (RPE) is a monolayer of cells with crucial role in the functioning of retina. Damages of the RPE and photoreceptors are leading causes of age related macular degeneration (AMD). It has been suggested that oxidative stress play an important role in the aetiology of AMD.
In this study we investigated the effect of EGCG on RPE cells viability and on cell antioxidant defence system. EGCG was delivered to the cells in different concentrations at sub confluence and incubated for 24 h. The oxidative stress was induced with 500 ?M hydrogen peroxide. The cells viability was determined by MTT assay, the inhibition of ROS generation by DCF-DA assay and the activity of glutathione peroxidise and superoxide dismutase by using commercial kits.
EGCG (50-100 ?M) treatment of human RPE cells in vitro has not detrimental effects on cells viability and determined an increase of viability when oxidative stress was induced by addition of hydrogen peroxide, showing a protective effect for mithocondrial enzymes. GPx activity was decreased by EGCG but SOD activity increased, both for cells with EGCG alone and cells treated with EGCG and H2O2. EGCG has an inhibitory effect on reactive oxygen species generation in the RPE cells by inhibiting the fluorescence of DCF. A diet rich in EGCG could contribute to the antioxidant protection and could have beneficial effects in the prevention of retinal degenerative diseases