Jurnal FKIP Universitas Mataram (Fakultas Keguruan Dan Ilmu Pendidikan)
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Primer Construction of Transporter Genes Based on Almt1 for The Analysis of Cmp-Sialic Acid Transporter Gene in Derendan
Get PDFDerendan (Lansium sp.) is an underutilized endemic fruit tree species from Bengkalis Island that grows in acidic peatland soils with high concentrations of toxic aluminum ions (Al³⁺). This study aimed to construct gene-specific primers to detect stress tolerance genes in derendan, with an initial focus on the ALMT1 (Aluminum-activated malate transporter) gene, which is known to mediate aluminum detoxification through organic acid exudation. Primers were designed based on ALMT1 sequences from model species and tested using PCR amplification and sequencing of derendan genomic DNA. Surprisingly, the amplified DNA fragment showed high similarity with CMP-sialic acid transporter 1 genes in several plant species, based on BLASTn analysis. The CMP-sialic acid transporter gene encodes a protein involved in the transport of sialic acid, an organic acid known to play roles in abiotic stress responses. These findings suggest that the constructed primers may target conserved transporter domains related to stress adaptation. The resulting primers have potential applications in molecular studies and breeding programs aimed at enhancing peatland stress tolerance in derendan and related species
The Effect of Basil Essential Oil Addition on the Functional Properties of Gelatin-Based Edible Film
Get PDFEdible film is a thin film that can be consumed immediately and can be used as a food packaging material. The incorporation of basil essential oil is anticipated to enhance the functional characteristics of edible film. The objective of this study is to evaluate the impact of incorporating basil essential oil on the water vapor transmission rate, gelation time, and the chemical structure of gelatin-based edible films. The research method employed a completely randomized design, incorporating three distinct treatments and three replications to ensure the robustness of the findings. The treatments comprised K1, which did not include the addition of basil essential oil; K2, which incorporated 4% basil essential oil; and K3, which incorporated 8% basil essential oil. The results demonstrated that the incorporation of basil essential oil led to a significant (P<0.05) increase in the WVTR value, with elevated concentrations resulting in increased WVTR. However, the incorporation of basil essential oil did not demonstrate a statistically significant impact on the gelation duration (P>0.05). FTIR analysis revealed an interaction between essential oil and gelatin that affected the film structure, thereby increasing the flexibility and antibacterial potential of the film. In conclusion, the incorporation of basil essential oil has been demonstrated to enhance the functional properties of gelatin-based edible films
Physical Stability Evaluation of Topical Formulation Containing Turmeric (Curcuma longa L.) Leaf Extract as a Potential Natural Repellent
Get PDFTurmeric (Curcuma longa L.) leaves, rich in bioactive compounds like tannins, terpenoids, and essential oils, have potential as a mosquito repellent for preventing vector-borne diseases such as dengue, filariasis, and malaria. This study aimed to evaluate the physical stability of repellent lotion formulations containing ethanol extract of turmeric leaves. The research was experimental in nature. Two formulations were prepared using extract concentrations of 10% (F1) and 20% (F2). The turmeric leaves were extracted using the maceration method with 96% ethanol, yielding 4.069%. The physical stability of the repellent lotion was tested using the freeze-thaw method over 12 days, evaluating organoleptic properties, homogeneity, pH, adhesion, and spreadability. Organoleptic testing showed color differences between the formulas. After stability testing, the consistency of the lotions became slightly thinner. Both formulations exhibited good homogeneity. The pH values were within the safe range for skin application. Changes in adhesion and spreadability were influenced by the testing process. In conclusion, the repellent lotion formulations containing turmeric leaf ethanol extract demonstrated good physical stability and met required quality standards
Analysis Relationship of The Current Velocity and Primary Productivity on Coral Reef in Bama Beach
Get PDFCoral reefs are one of the ecosystems with the highest biodiversity. The purpose of this study was to determine the relationship of current velocity has on primary productivity in coral reef ecosystems. Current velocity measurements using a current meter and primary productivity measurements using the Winkler method by ƒincubated for 5 hours. The results obtained from this study are that current velocity has an effect on primary productivity in coral reef ecosystems, R value 0.895 and R Square shows 80.1%. The significance value obtained Sig = 0,04, which means less than 0.05, it can be interpreted that the effect of current velocity on primary productivity is strong category. Based on the study results obtained, more research is necessary to find out how much the velocity of the current affects the primary productivity of the coral reef
Marketing Strategy of Mushroom Baglog in UMKM Agro Jamur Lombok, Gunungsari Sub-District, West Lombok
Get PDFWhite oyster mushroom is one of the most widely cultivated mushrooms in Indonesia because it has a high economic value. Umkm Agro Mushroom Lombok is one of the oyster mushroom and mushroom baglog cultivation businesses in West Lombok district. Apart from oyster mushrooms, in Umkm, mushroom baglogs also have a fairly high economic value with the right marketing and marketing strategy. One of the analytical tools that can be used in designing a marketing strategy is SWOT analysis. This analysis is able to identify strengths, weaknesses, opportunities and threats. The method in this study uses descriptive methods with data sources that come from direct sources or primary data and come from other sources or secondary data. The results of the analysis using SWOT show that quality and service are the strengths of Agro Mushroom Lombok, while the lack of promotion and baglogs that are vulnerable to weather are weaknesses, the potential that can be utilized is customer loyalty and a fairly wide market reach. The threats faced are the emergence of new competitors and limited raw materials. The strategies that can be applied are by prioritizing product quality so that the level of customer loyalty increases, besides that it is necessary to intensify promotion through social media to increase market reach
Mangrove Diversity in Pare Mas Jerowaru, East Lombok
Get PDFThe purpose of study is to determine the diversity of mangrove species in Pare Mas Jerowaru East Lombok. Data collection in this study was carried out using the drone check method and a combination of line and square transect methods measuring 2 x 2 m (seedling category), 5 x 5 m (pile category), 10 m x 10 (pole category), and 20 m x 20 m (tree category). Data analysis was carried out by calculating frequency, density, closure area, important value index, Shanon-Wiener diversity index (H'), uniformity index and dominance index. Mangrove species found in the mangrove area in Pare Mas are 233 individuals, consisting of 3 species belonging to 3 families. The value of mangrove species diversity index in mangrove area in Pare Mas, Jerowaru, East Lombok is 0.743, uniformity index value is 0.676, dominance index value is 0.507, of 3 research transects of Rhizophora Apiculata and Sonneratia Alba species have highest frequency value of 0.8 ind/m². The highest density is owned by seedling category of 1,708 ind/m2, followed by sapling category of 1,187 ind/m2. Conclusion that the value of the mangrove species diversity index in Pare Mas Village is in the low category of 0.743. The highest species diversity index was found in transect 2 with a diversity index value of 0.797 and lowest in transect 3, which was with a value of 0.642
The Influence of PSB Application on the Growth of Aloe vera in a Verticulture Cultivation System
Get PDFAloe vera is a tropical plant with significant potential to develop into a promising agricultural sector in Indonesia. To achieve high-quality Aloe vera harvests, it is important to pay attention to several growth conditions, especially plant care such as fertilization. This study aims to determine the effect of Photosynthetic Bacteria (PSB) fertilizer application and the effective dosage of PSB fertilizer for the growth of Aloe vera using vertical farming techniques. This research used a Non-Factorial Randomized Block Design (RBD), with treatment factors including: no application of PSB fertilizer (P0), PSB 5 ml/l (P1), PSB 15 ml/l (P2), and PSB 30 ml/l (P3). The research involved several stages of observation, including plant height, number of leaves, leaf width, leaf thickness, and root volume. The observed data were analyzed using ANOVA with the help of SPSS 26. If the ANOVA test showed a significant effect, further analysis was carried out using Duncan's Multiple Range Test (DMRT). The results showed that the application of PSB fertilizer had an effect on plant height (22.8 cm), leaf width (2.76 cm), number of leaves (7 leaves), leaf thickness (8.91 cm), and root volume (4 cm³). The best average treatment result for Aloe vera growth was obtained at a dosage of 15 ml/l (P2), indicating that Photosynthetic Bacteria (PSB) fertilizer at a dosage of 15 ml/l (P2) was sufficient to supply the necessary nutrients for the growth of Aloe vera
The Effect of Adding Kaliandra Leaf Flour on Broiler Chicken Performance
Get PDFSince broiler production is one of the sources of animal protein that the community consumes in large quantities, efforts are being made to improve its quality and efficiency through the addition of alternative feed ingredients. One potential feed item that can be used is kalandra flour, which is known to have bioactive compounds that can impact the performance and body composition of broiler chickens. The purpose of this study was to determine the effect of adding calliandra leaf flour on the performance of broiler chickens. This study used a completely randomized design (CRD) with four treatments and four replications. There were four broiler chicks in each replication, for a total of 64 chicks. The study treatments were: 100% control feed was used at P0, 100% control feed plus 1% calliandra leaf meal was used at P1, 100% control feed plus 3% calliandra leaf meal was used at P2, and 100% control feed plus 5% calliandra leaf meal was used at P3. Feed conversion, average daily weight gain, and feed consumption were the observed variables. There was no visible variation in the study findings for feed consumption, average daily weight gain, and feed conversion (P>0.05). According to the study findings, broiler performance was not significantly affected by the addition of calliandra meal up to 5%
Gastropod Community Structure as a Bioindicator of Water Quality n The Seagrass Ecosystem at Samuh Beach, Bali
Get PDFCoastal ecosystems, such as the seagrass meadows at Samuh Beach, Bali, are vulnerable to anthropogenic pressures from high-intensity tourism. This degradation necessitates effective monitoring tools, and gastropod communities are potential bioindicators due to their sensitivity to environmental change. The objective of this study was to evaluate the gastropod community structure as a bioindicator of water quality within this ecosystem. The study was conducted from November to December 2024 at two stations representing different levels of human activity, using line transects and quadrat sampling. A total of 10 gastropod species were recorded. The community structure was characterized by low species diversity (H' = 1.25), moderate evenness (E = 0.52), and low dominance (C = 0.35). In contrast, all measured water quality parameters were found to be within optimal ranges for marine biota. The dissonance between the low biodiversity and favorable water chemistry suggests that the gastropod community is responding to unmeasured stressors, likely physical disturbances or specific pollutants associated with tourism. This study validates the efficacy of using gastropod assemblages as sensitive bioindicators of ecosystem health, capable of detecting impacts that standard water quality tests may miss. Continuous monitoring is recommended to safeguard the area
The Role of Manuka Honey in Protecting Against Cochlear Hair Cell Damage Caused by Diabetes Mellitus in Rats, Assessed Through Otoacoustic Emission
Get PDFDiabetes mellitus is an escalating public health concern worldwide, with Indonesia being one of the countries heavily affected and facing a projected increase in cases. This condition is known to cause a range of complications, including hearing loss, which is frequently observed in diabetic individuals. This study aims to investigate the potential protective effect of honey against cochlear damage induced by diabetes mellitus. This ex-vivo experimental study applied a pretest-posttest control group design. Conducted over five months at the FMIPA USU Animal Laboratory, the study involved 25 healthy adult male Rattus norvegicus Wistar rats (150–250 grams, aged 2–3 months). The rats were divided into three treatment groups and assessed before and after honey administration on days 0, 3, 6, 9, and 12. SNR value differences were analyzed using Repeated Measures ANOVA or the Friedman test. Administration of honey at a dose of 1 g/kg body weight was more effective in preventing the decline of SNR values in diabetic rat models compared to the 2 g/kg dose. Honey demonstrates a protective effect against cochlear hair cell damage in diabetic Rattus norvegicus, as evidenced by SNR values from OAE assessments