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    Allele frequency for the SC x AB cross

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    Allele frequencies, following a sliding window averaging of 3000 consecutive SNPs, across the 12 chromosomes of F2 hybrids from crosses between the Santa Cruz (SC) and Abalone Cover (AB) populations of Tigriopus californicus. SA refers to the SCf x ABm cross, and AS is the ABf x SCm cross

    McFadden_suppl_170taxa_concatenated_alignment

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    Alignment of concatenated mtMutS + igr1 + COI + 28S rDNA barcodes for 170 taxa used to construct maximum likelihood tree. Taxon abbreviations can be found in Appendix S1, Table S1 of the publication

    Data from: Evolution of an insect immune barrier through horizontal gene transfer mediated by a parasitic wasp

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    Genome sequencing data have recently demonstrated that eukaryote evolution has been remarkably influenced by the acquisition of a large number of genes by horizontal gene transfer (HGT) across different kingdoms. However, in depth-studies on the physiological traits conferred by these accidental DNA acquisitions are largely lacking. Here we elucidate the functional role of Sl gasmin, a gene of a symbiotic virus of a parasitic wasp that has been transferred to an ancestor of the moth species Spodoptera littoralis and domesticated. This gene is highly expressed in circulating immune cells (haemocytes) of larval stages, where its transcription is rapidly boosted by injection of microorganisms into the body cavity. RNAi silencing of Sl gasmin generated a phenotype characterized by a precocious suppression of phagocytic activity by haemocytes, which was rescued when these immune cells were incubated in plasma samples of control larvae, containing high levels of the encoded protein. Proteomic analysis demonstrated that the protein Sl gasmin is released by haemocytes into the haemolymph, where it opsonizes the invading bacteria to promote their phagocytosis, both in vitro and in vivo. Our results show that important physiological traits do not necessarily originate from evolution of pre-existing genes, but can be acquired by HGT events, through unique pathways of symbiotic evolution. These findings indicate that insects can paradoxically acquire selective advantages with the help of their natural enemies

    3) Phenological data

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    3-a) Date: the date of data collection 3-b) Month: the month in number 3-c) Season: Long dry season, long rainy season, short dry season, short rainy season 3-e) Plant ID: the unique identifier of each individual plant 3-f) Species: the scientific name of the species 3-g) Family: Family names 3-h) DBH: the diameter at breast height of the individual plant (in centimetre) 3-i) Basal area (in centimetre square) 3-j) Fruit score: 0 (none), 1 (few), 2 (many

    AdehabitatHR R code

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    R code to install the 'AdehabitatHR' package, then run the code on the 'GAI_Home_range_overlap_Adehabitat_input.xls'. See 'README' text file for more detail

    data availability

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    WisAsp fam file

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    WisAsp fam file for PLINK analyses

    SPPA of Picea abies mortality

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    The file consists of four sheets which present SPPA of mortality (p11{r}; p12{r} and g1,1+2 - g2,1+2 {r}) in years 1993-2013, 1993-2003 and 2003-2013:(1) overall mortality; (2) deer-caused mortality; (3) mortality due to mechanical causes and (4) mortality of unknown causes

    Supplementary Appendix

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    This file explains the math described in the paper

    Supplementary Table 2

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    Mesowear data of late Pleistocene equid and bison samples studied

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