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MPS raw data
No full textBarcodes and primer sequences are available in the mapping file formatted for QIIME
Data from: Nitrogen addition pulse has minimal effect in big sagebrush (Artemisia tridentata) communities on the Pinedale Anticline, Wyoming (USA)
No full textNitrogen additions are known to elicit variable responses in semi-arid ecosystems, with responses increasing with precipitation. The response of semi-arid ecosystems to nitrogen are important to understand due to their large spatial extent worldwide and the global trend of increasingly available nitrogen. In this study, we evaluated the impact of a single nitrogen addition pulse on a semi-arid big sagebrush (Artemisia tridentata) ecosystem in western Wyoming. This is important given that sagebrush ecosystems are poorly understood, despite their prevalence in the western US. In addition, large-scale nitrogen additions have begun on sagebrush landscapes in Wyoming in order to mitigate population declines in mule deer (Odocoileus hemionus). The study objectives were (1) to evaluate the effectiveness of a nitrogen fertilization pulse in increasing sagebrush biomass and forage quality, and (2) to assess effects of nitrogen addition on soil biogeochemistry and vegetation community structure. We fertilized 15 plots across 5 locations in western Wyoming using a single pulse of urea (5.47g N m-2). In addition, we immobilized available nitrogen through surface hay treatments (254g hay/m2). Nitrogen additions failed to increase growth of sagebrush, alter nitrogen content of sagebrush leaders, or alter greenhouse gas efflux from soils. The plant community also remained unchanged; total cover, species richness, and community composition were all unaffected by our treatment application. Over the two years of this study, we did not find indications of nitrogen limitation of ecosystem processes, despite a wet growing season in 2014. Thus, we have found a general lack of response to nitrogen in sagebrush ecosystems and no treatment effect of a single pulse of N to sagebrush biomass or forage quality
Supplementary material S3
No full textWhole set of images used for landmark capture for the geometric morphometrics analyses
teak_repr_hc_models_HiC_con_sorted_modiGeneID.gff
No full textGFF of representative high-confidence gene model
teak_working_gene_fpkm_matrix_con_sorted.txt
No full textExpression abundances of the working gene set were estimated using cufflinks RNAseq experiment atlas from NCBI SRA BioProject PRJNA28760
Baden_VvNesting_Total_nest_locations
No full textUTM locations for all nest and park sites included in this study, along with the identity for who constructed nest sites, whether nest/park sites were used, and if used, whether sites were used singly or communally, and the number and identities of users
Metabarcoding data
No full textotu.fa: a FASTA format file containing the sequences of all of the OTUs generated by the study; map.csv: a table reporting the number of reads of each OTU in each sequenced library (well); summary_metadata.csv: a table reporting the sample data for each wel
Data from the stickleback experiment (Kim et al. 2018)
No full textAn Excel file with the experimental data. Each parameter is explained as a comment in the column title