Acta Fytotechnica et Zootechnica Online (Faculty of Agrobiology and Food Sciences, Slovak University of Agriculture in Nitra)
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IRAP MARKERS OF PLUM TRIM RETROTRANSPOSON
Full text linkThe efficiency comparison of two IRAP markers of plum (Prunus domestica L.) genome was realized on nine plum genotypes. PrimerIRAP1 was derived from LTR´s (Long Terminal Repeat) 3´end of plum retrotransposon Cassandra and primer IRAP2 was designed based onLTR´s 5´end of the same retrotransposon. Orientation and length of primers were designed as the same. PCR products were amplified inoptimized conditions. By the study of electrophoreograms it have been revealed that IRAP1 primer provided 4 polymorphic levels from 10total levels and IRAP2 primer created 10 polymorphic levels from 18 total levels. In the case of IRAP2 primer there were registered uniquebands, too. Percentage content of polymorphic fragments constitutes 23% for IRAP1 and 41% for IRAP2 primer. Four plum genotypescollected from Slovak locations of their natural occurrence (Pečovská N. Ves, Torysa, Podolínec and Lipany) were evaluated based ongenetic similarity Nei, Li coefficients as the most similar by both primers
EFFECT OF BIO-ORGANIC FERTILIZERS ON SEASONAL CHANGES OF INORGANIC NITROGEN FORMS IN THE SOIL UNDER WINTER WHEAT
No full textIn a field stationary experiment was during growing seasons 2007/2008 and 2008/2009 investigated the effect of bio-organic fertilizers Condit Mineral and Condit Eco on the dynamics of inorganic nitrogen in the soil under winter wheat. The results of our experiment confirmed the greater dependence on the dynamics of inorganic nitrogen in the soil had climate conditions during the growing season than the applied fertilizers. Differences between fertilized variants were statistically no-significant. The highest contents of inorganic nitrogen were in the autumn Condit Mineral fertilizer application