Centro de Investigación y Tecnología Agroalimentaria de Aragón
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Variabilidad genotípica y anual de la composición química del aceite de nuez de plantones de nogal del Alto Atlas
Full text linkProtein and oil content, fatty acid composition and tocopherol concentration were determined for two years in the kernel of ten candidate walnut selections from the high Atlas Mountains in Morocco. Considerable variation between genotypes was found for all parameters. The ranges of protein content (11.58-14.5% of kernel dry weight, DW), oil content (54.4-67.48% of kernel DW), oleic (12.47-22.01% of total oil), linoleic (55.03-60.01%), linolenic (9.3-15.87%), palmitic (6.84-9.12%), and stearic (1.7-2.92%) acid percentages, gamma-tocopherol (188.1-230.7 mg.kg(-1) of oil), delta-tocopherol (23.3-43.4 mg.kg(-1)), and alpha-tocopherol (8.9-16.57 mg.kg(-1)) contents agreed with previous results obtained from other commercial walnut cultivars. The effect of year was significant for all the chemical components, except for oil content and palmitic acid percentage. Some genotypes showed high oil contents and consistently high values of gamma-tocopherol in both years of study. The introduction of these genotypes as new cultivars by vegetative propagation may result in a an increase in quality of the walnuts from the high Atlas Mountains of Morocco, and as a seed source for forest walnut propagation in the same region.Se determinó durante dos años el contenido en proteína y aceite, la composición en ácidos grasos y la concentración en tocoferoles en la pepita de diez plantones de nogal seleccionados en el Alto Atlas de
Marruecos, encontrándose una considerable variación entre genotipos para todos los parámetros. Los rangos
del contenido en proteína (11.58–14.5 % del peso seco, PS), contenido en aceite (54.4–67.48 % PS), porcentaje
de ácido oleico (12.47–22.01% del aceite total), linoleico (55.03–60.01 %), linolénico (9.3–15.87 %), palmítico
(6.84–9.12 %), y esteárico (1.7–2.92 %), contenido en γ-tocoferol (188.1–230.7 mg·kg−1de aceite), δ-tocoferol
(23.3–43.4 mg·kg−1) y α-tocoferol (8.9–16.57 mg·kg−1), coincidieron con resultados previos de otros cultivares
comerciales de nogal. El efecto del año fue significativo para todos los componentes químicos, excepto para el
contenido en aceite y el porcentaje de ácido palmítico. Algunos genotipos destacaron por un elevado contenido
en aceite y valores consistentes de γ-tocoferol en ambos años del estudio. Estos genotipos muestran interés para
su propagación vegetativa como nuevos cultivares para aumentar la calidad de las nueces del Alto Atlas, así
como fuente de semillas para la propagación forestal del nogal en la misma regiónThis research was funded by the grants of “Healthy Food for life” program Marie Curie IRSES, and the Research Group A12 of Aragón.Fatty acid profileGenetic resourcesOil contentProtein contentTocopherol contentWalnutContenido en aceiteContenido en proteínaContenido en tocoferolesNogalPerfil de ácidos grasosRecursos genéticosPublishe
Pilowred®, a Nematode-resistant and Low-vigor Rootstock for Prunus
Full text linkThe demands of the fruit sector in the Mediterranean Basin have shifted in recent years, largely as a result of edaphoclimatic limitations resulting from climate change. These limitations include water scarcity during extreme drought periods, warmer cold seasons, the prevalence of soil diseases such as those related to the presence of root-knot nematodes (RKNs), and iron chlorosis. To address these issues, the Prunus spp. rootstock breeding program at the Agrifood Research and Technology Centre of Aragon (CITA) developed red-leaf hybrids from the ‘Garfi’ × ‘Nemared’ series (G × N): Garnem®, Felinem®, and Monegro® (Felipe 2009). In addition, CITA selected the plum ‘Montizo’ as a clonal rootstock, which adapts to wet and heavy soils, reducing tree size compared with other rootstocks (Felipe et al. 1997). In recent years, the adoption of more intensified and technologically advanced cultivation systems has increased the need for low-vigor rootstocks to facilitate sustainable orchard management. In response to these demands, Pilowred® has been released as a valuable rootstock for several stone fruit species, particularly almond. One of its more notable features is its low vigor, which enhances the productivity of the grafted cultivar, leading to greater yields in the orchard. In addition, it exhibits easy vegetative propagation and good graft compatibility. It requires a shorter winter dormancy period, facilitating a rapid entry into production, and it shows greater water use efficiency compared with other G × N hybrids.almond × peach hybridin vitro propagationMeloidogyne sppnematode resistancered leafPublishe
Role of egg white protein gelling capacity on the processability and properties of compression-moulded films
Full text linkThis study aimed to assess the impact of the gelling capacity of powdered egg white protein (EWP) on the processability and properties of compressed-moulded films. Three commercial grades of EWP with varying gelling capacities and three protein-to-plasticiser ratios were selected for film preparation. The EWP structure was characterised by FTIR and XRD, and the relative abundance of proteins was estimated using LC-ESI-MS/MS. The rheological properties of the film-forming solutions (FFS), along with the mechanical, barrier, and optical properties of resulting films, were also evaluated. The EWP samples exhibited differences in secondary structure, soluble protein content, and protein profile, which were reflected in the viscosity, consistency, and elastic modulus of the FFS, all of which increased with gelling capacity. The processability and stability of the FFS were found to depend on the appropriate combination of gel strength and protein-to-plasticiser ratio. In addition to the protein-to-plasticiser ratio, the ?-turn abundance in the secondary structure of the EWP film was the parameter most strongly correlated with Young's modulus (p<0.01). However, the barrier properties of the films were significantly influenced solely by the protein-to-plasticiser ratio (p<0.01), with higher plasticiser content resulting in increased oxygen and water vapour permeability.Edible filmmolecular structurefood packagingegg white proteinglycerolcompression mouldingbarrier propertiesPublishe
Influence of Summer Drought on Resprouting and Leaf Senescence in a Common Garden of Prunus spinosa L.
Full text linkUnderstanding how woody plants cope with severe water shortages is critical, especially
for regions where droughts are becoming more frequent and intense. We studied the effects of
drought intensity, focusing on post-drought resprouting, autumn leaf senescence and the subsequent
spring bud burst. Furthermore, we aimed to study population differentiation in the drought and postdrought responses. We performed a summer dry-out experiment in a common garden of potted
Prunus spinosa L. (Rosaceae) saplings. We looked at the responses in different visual stress symptom
categories and at provenance differentiation between a local (Western Europe), a more southern and
a more northern provenance. The chance on post-drought resprouting was larger for the more
severely affected plants, and the timing of resprouting was earlier than for the less severely affected
ones. The plants that displayed wilting of the leaves during the drought had a leaf senescence 2.7
days earlier than the controls, whereas plants with 25 to 75% and more than 75% of desiccated leaves
were 7 and 15 days later, respectively. During the drought, the local provenance was the first to
develop visual symptoms. Still, for the plants with no to mild symptoms due to the drought, this
provenance displayed a larger chance for post-drought resprouting. Among the control plants, the
northern provenance was earlier, and the southern was later in leaf senescence compared to the local
provenance. This differentiation disappeared for the plants with more than 25% of desiccated leaves
due to the drought. Whereas leaf senescence could be earlier or later depending on the developed
drought symptoms, the timing of bud burst was only delayed. Results indicate that resprouting and
timing of leaf senescence are responsive to the severity of the experienced drought in a provenance
dependent waywater limitationdry-out experimentleaf discolorationblack thornrecoveryphenologybud burstprovenance trialPublishe
Presentación del proyecto RECONECTA
No full textRECONECTA cuenta con el apoyo de la Fundación Biodiversidad del Ministerio para la Transición Ecológica y Reto Demográfico (MITECO) en el Marco del Plan de Recuperación, Transformación y Resilencia (PRTR), financiado por la Unión Europea- NextGenerationU
Efecto del manejo agronómico y el uso de cubiertas vegetales sobre indicadores de calidad del suelo en cultivos de especial relevancia en Aragón
Full text linkEl estudio forma parte del programa AGROALNEXT, promovido por MCIN
y financiado por la Unión Europea NextGenerationEU (PRTR-C17.I1)
Consumer preferences for nutritional claims: An exploration of attention and choice based on an eye-tracking choice experiment
Full text linkNutritional claim (NC) requirements on food packages are among the most important and influential EU policy measures related to diet and have the capacity to promote healthy eating. This study combines a discrete choice experiment (DCE) method with eye-tracking (ET) technology to assess consumer preferences for multiple NCs in yogurt selection and explores the relationships between the NC preferences and the visual attention paid to these claims and the visual attention and choice decisions. The results indicate that the low-sugar NC was the least-preferred claim in all the models. Overall, the presence of NCs generally increases visual attention in terms of fixation count, which may be linked to an increased likelihood of affecting the final decision to purchase yogurts with NCs.This work was funded by the Spanish National Institute of Agriculture, Food Research, and Technology: INIA RTA 2013-0092-00-00 “Comportamiento del consumidor en la compra de alimentos con alegaciones nutricionales y/o de salud.” The authors thank the editor, Anderson de Souza Sant'Ana,and two anonymous journal reviewers for their valuable comments and
suggestions, which have helped us to improve the quality of the paper significantly. We also thank Kessels Roselinde for her valuable opinions and suggestions related to the choice design.Nutritional claimeye trackingchoiceyogurtconsumerPublishe
SNP rs403212791 in exon 2 of the MTNR1A gene is associated with reproductive seasonality in the Rasa aragonesa sheep breed.
Full text linkThe aim of this study was to characterize and identify causative SNPs in the MTNR1A gene responsible for the reproductive seasonality traits in the Rasa aragonesa sheep breed. A total of 290 ewes (155, 84 and 51 mature, young and ewe lambs, respectively) from one flock were controlled from January to August. The following three reproductive seasonality traits were considered: the total days of anoestrus (TDA) and the progesterone cycling months (P4CM); both ovarian function seasonality traits based on blood progesterone levels; and the oestrus cycling months (OCM) based on oestrous detection, which indicate behavioural signs of oestrous. We have sequenced the total coding region plus 733 and 251 bp from the promoter and 3'-UTR regions, respectively, from the gene in 268 ewes. We found 9 and 4 SNPs associated with seasonality traits in the promoter (for TDA and P4CM) and exon 2 (for the three traits), respectively. The SNPs located in the gene promoter modify the putative binding sites for various trans-acting factors. In exon 2, two synonymous SNPs affect RFLP sites, rs406779174/RsaI (for the three traits) and rs430181568/MnlI (for OCM), and they have been related with seasonal reproductive activity in previous association studies with other breeds. SNP rs400830807, which is located in the 3'-UTR, was associated with the three traits, but this did not modify the putative target sites for ovine miRNAs according to in silico predictions. Finally, the SNP rs403212791 (NW_014639035.1: g.15099004G > A), which is also associated with the three seasonality phenotypes, was the most significant SNP detected in this study and was a non-synonymous polymorphism, leading a change from an Arginine to a Cysteine (R336C). Haplotype analyses confirmed the association results and showed that the effects found for the seasonality traits were caused by the SNPs located in exon 2. We have demonstrated that the T allele in the SNP rs403212791 in the MNTR1A gene is associated with a lower TDA and higher P4CM and OCM values in the Rasa Aragonesa breed.Copyright © 2018 Elsevier Inc. All rights reserved.This study was partially financed by the Ministry of Economy and Competitiveness research projects INNPACTO IPT-010000-2010-33 and INIA-RTA2013-00041 and the European Union Regional Development funds (and the Research Group Funds of the Aragón Government, A13 and A49). We would like to thank Grupo Pastores for providing the experimental animals and facilities for the study.sheepoestrous behaviourovulatory activityMTNR1ASNPPublishe
