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Photoinduced Charge-Transfer Dynamics in Fluorescent Electron Donor-Acceptor Polymers
No full textData presented in: 'Photoinduced Charge-Transfer Dynamics in Fluorescent Electron Donor-Acceptor Polymers', E. Sucre-Rosales, S. Ye, Y. Bao and E. Vauthey, Chem. Sci
Loss of Fsr quorum sensing promotes biofilm formation and worsens outcomes in enterococcal infective endocarditis - GC-MS Dataset
No full textRaw GC-MS Data associated with the manuscript 'Loss of Fsr quorum sensing promotes biofilm formation and worsens outcomes in enterococcal infective endocarditis' by Antypas et al.
Infective endocarditis (IE) is a life-threatening biofilm-associated infection, yet the factors driving biofilm formation in this setting remain poorly understood. Here, we identified the Fsr quorum sensing (QS) system of Enterococcus faecalis as a potent negative regulator of IE pathogenesis. Using microfluidic and in vivo models, we show that Fsr is induced in late IE when bacteria become shielded from blood flow. Deleting Fsr promoted robust biofilm growth, partly through downregulation of gelE and sprE, reprogrammed metabolism by upregulating lrgAB to enhance pyruvate utilization, and increased gentamicin tolerance in vivo. Furthermore, the Fsr-regulated protease GelE cleaved human pro-IL-1β into a bioactive fragment, suggesting a potential species-specific mechanism for inflammation modulation by QS. Consistent with our pre-clinical findings, analysis of two IE patient cohorts linked naturally occurring Fsr-deficient E. faecalis to prolonged bacteremia and a higher disease severity score. Overall, our findings provide insights into the role of QS in biofilm growth and persistence in IE.
This dataset contains the raw data of the gas chromatography-mass spectrometry (GC-MS) analysis, quantifying lactic acid, pyruvic acid and L-alanine in cell pellets (CL) and supernatant (SN) of OG1RF WT, Δfsr, lrgA::Tn, and lrgB::Tn cultured under aerobic (AER) and anaerobic (ANA) conditions.
Details of the analyses including sample harvest, metabolite extraction, derivatization, GC-MS settings and metabolite quantification are described in the Material and Methods section of the manuscript.
The data was acquired with a 8890 GC System (Agilent Technologies) equipped with a DB5 capillary column (J&W Scientific, 30 m, 250 μm inner diameter, 0.25-μm film thickness, 10-m inert duraguard) connected to a 5977B GC/MSD in electron impact (EI) mode linked to a 7693A autosampler (Agilent Technologies). The files are .D format
Décimas nuevas de la sagrada pasión: Digital edition
No full textDigital edition of a Spanish pliego de cordel, published by the Andalusian printer José María Moreno (19th c.). It contains the XML-TEI file of the edition, as well as the files that led to this edition, i.e. the digital facsimiles (raw and retouched), and the transcription produced by the OCR tool Transkribus (PAGE-XML). This digital edition is a part of the research project Desenrollando el cordel / Untangling the cordel / Démêler le cordel (2020-2024), led by professor Constance Carta at the University of Geneva and supported by the philanthropic Sandoz-Monique de Meuron Family Foundation
El cantor de las hermosas: Digital Edition
No full textDigital edition of a Spanish pliego de cordel (19th c.). It contains the XML-TEI file of the edition, as well as the files that led to this edition, i.e. the digital facsimiles (raw and retouched), and the transcription produced by the OCR tool eScriptorium (ALTO-XML). This digital edition is a part of the research project Desenrollando el cordel / Untangling the cordel / Démêler le cordel (2020-2024), led by professor Constance Carta at the University of Geneva and supported by the philanthropic Sandoz-Monique de Meuron Family Foundation
Blas de leon: Digital Edition
No full textDigital edition of a Spanish pliego de cordel (19th c.). It contains the XML-TEI file of the edition, as well as the files that led to this edition, i.e. the digital facsimiles (raw and retouched), and the transcription produced by the OCR tool eScriptorium (ALTO-XML). This digital edition is a part of the research project Desenrollando el cordel / Untangling the cordel / Démêler le cordel (2020-2024), led by professor Constance Carta at the University of Geneva and supported by the philanthropic Sandoz-Monique de Meuron Family Foundation
La Enamorada de Cristo María Jesus de Gracia
No full textDigital edition of a Spanish pliego de cordel, published by the Andalusian printer José María Moreno (19th c.). It contains the XML-TEI file of the edition, as well as the files that led to this edition, i.e. the digital facsimiles (raw and retouched), and the transcription produced by the OCR tool Transkribus (PAGE-XML). This digital edition is a part of the research project Desenrollando el cordel / Untangling the cordel / Démêler le cordel (2020-2024), led by professor Constance Carta at the University of Geneva and supported by the philanthropic Sandoz-Monique de Meuron Family Foundation
Relación en la que declara el chiste mus alegre y divertido que pasó entre un viejo llamado Tio Facundo y un caballero llamado D. Claudio: Digital Edition
No full textDigital edition of a Spanish pliego de cordel (19th c.). It contains the XML-TEI file of the edition, as well as the files that led to this edition, i.e. the digital facsimiles (raw and retouched), and the transcription produced by the OCR tool eScriptorium (ALTO-XML). This digital edition is a part of the research project Desenrollando el cordel / Untangling the cordel / Démêler le cordel (2020-2024), led by professor Constance Carta at the University of Geneva and supported by the philanthropic Sandoz-Monique de Meuron Family Foundation
Hechos gloriosos de nuestro ejército en los campos de África: Digital Edition
No full textDigital edition of a Spanish pliego de cordel (19th c.). It contains the XML-TEI file of the edition, as well as the files that led to this edition, i.e. the digital facsimiles (raw and retouched), and the transcription produced by the OCR tool eScriptorium (ALTO-XML). This digital edition is a part of the research project Desenrollando el cordel / Untangling the cordel / Démêler le cordel (2020-2024), led by professor Constance Carta at the University of Geneva and supported by the philanthropic Sandoz-Monique de Meuron Family Foundation
Villancicos alegres para cantar en seguidillas: Digital Edition
No full textDigital edition of a Spanish pliego de cordel (19th c.). It contains the XML-TEI file of the edition, as well as the files that led to this edition, i.e. the digital facsimiles (raw and retouched), and the transcription produced by the OCR tool eScriptorium (ALTO-XML). This digital edition is a part of the research project Desenrollando el cordel / Untangling the cordel / Démêler le cordel (2020-2024), led by professor Constance Carta at the University of Geneva and supported by the philanthropic Sandoz-Monique de Meuron Family Foundation
Las ligas de mi morena: Digital Edition
No full textDigital edition of a Spanish pliego de cordel (19th c.). It contains the XML-TEI file of the edition, as well as the files that led to this edition, i.e. the digital facsimiles (raw and retouched), and the transcription produced by the OCR tool eScriptorium (ALTO-XML). This digital edition is a part of the research project Desenrollando el cordel / Untangling the cordel / Démêler le cordel (2020-2024), led by professor Constance Carta at the University of Geneva and supported by the philanthropic Sandoz-Monique de Meuron Family Foundation