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Analysis of the developmental stages, kinetics, and phenotypes exhibited by myeloid cells driven by GM-CSF in vitro
Get PDFThe developmental progression of conventional DC has been quite well defined, yet the developmental pathway of monocyte-derived, GM-CSF-driven DC is less well understood. We addressed this issue by establishing an isolation strategy that identifies five distinct GMCSF derived cell types. Expression of Ly6C and CD115 (Csf-1R) was used to identify and isolate four populations. One of the populations could be further separated based on CD11c expression, distinguishing five populations. We further defined these cells based on expression of transcription factors and markers of early and later stages of myeloid development. These discreet developmental stages corresponded well with previously defined populations: Common Myeloid Progenitors (CMP), Granulocyte/Macrophage Progenitors (GMP), Monocytes, as well as Monocyte-derived macrophages (moMac) and Monocyte-derived DC (moDC). Finally, within the moMac population we also identified moDC precursor activity (moDP) that could be distinguished from moMac and moDC based on their level of MHC class II expression and developmental plasticity.PublishedYe
RBD Library Symposium
Get PDFPanel presentation given on the occasion of the dedication of the Mell Classroom Building on September 1, 2017.N
Navigating the Open Access Landscape
No full textThis talk was given during Open Access Week to raise awareness of issues in OA. Open access became important initially due to a sharp rise in journal costs while library budgets remained flat, even as the Internet greatly reduced the marginal cost of providing access to information. The “green” and “gold” models of open access are summarized, and finally issues of quality in open access, including “predatory” journals, are explored
Guide for Dissecting the Dog, A
Get PDFA Guide for Dissecting the Dog has many distinctive features designed to aid students in their study of gross anatomy. In keeping with Dr. Fletcher’s original concept, descriptive material is minimal and most illustrations and photographs are designed to facilitate the dissection. Dependence on the excellent figures and descriptions in anatomy texts such as Miller’s Anatomy of the Dog by Dr. Howard Evans or Textbook of Veterinary Anatomy by Dyce, Sack and Wensing is by design. We have included several conceptual sketches that present ideas not available elsewhere in veterinary literature. When anatomical terms are introduced, they are underlined. Dissection instructions are presented in bold blue print and brief clinical comments are presented in italicized red print. Students dissect the male and female alternately so they can view and study structures on each sex before the specimens are altered or destroyed by subsequent dissection. The nomenclature throughout is that of Nomina Anatomica Veterinaria. The entire dissection sequence presented in A Guide for Dissecting the Dog can be completed in 96 hours. This book is a work in progress and will be updated periodically. With text by Thomas F. Fletcher, Paul F. Rumph, Phillip D. Garrett and Gerald R. Bratton; drawings by Phillip D. Garrett; and photos by Elizabeth A. Lantz, Elaine S. Coleman, D. Ray Wilhite and Paul F. Rumph. Compiled by Paul F. Rumph, DVM, MS
Scalings for the Alfven-cyclotron instability: Linear dispersion theory and hybrid particle-in-cell simulations
Get PDFThe Alfven-cyclotron instability is driven by an ion temperature anisotropy such that T-perpendicular to/T-|| >1 where and || denote directions perpendicular and parallel to a uniform background magnetic field B-o, respectively. The computations presented here consider a model of a magnetized, homogeneous, collisionless plasma. Two representations of the proton velocity distribution are considered: a single bi-Maxwellian and a magnetospheric-like configuration of two components, a more dense, relatively cool, isotropic component and a less dense, relatively hot, bi-Maxwellian component which drives the instability. Only wave propagation parallel to B-o is considered. Using numerical solutions of the full kinetic linear dispersion equation, concise analytic expressions for the scaling of the dimensionless maximum instability growth rate and the corresponding dimensionless real frequency are derived as functions of three dimensionless variables: the hot proton temperature anisotropy, the relative hot proton density, and the hot proton (||). Furthermore, using one-dimensional hybrid particle-in-cell simulations of this same instability, a third relation for the scaling of the maximum amplitude of the dimensionless fluctuating magnetic field energy density is derived.PublishedYe
Phylogenomic analyses of Crassiclitellata support major Northern and Southern Hemisphere clades and a Pangaean origin for earthworms
No full textBACKGROUND: Earthworms (Crassiclitellata) are a diverse group of annelids of substantial ecological and economic importance. Earthworms are primarily terrestrial infaunal animals, and as such are probably rather poor natural dispersers. Therefore, the near global distribution of earthworms reflects an old and likely complex evolutionary history. Despite a long-standing interest in Crassiclitellata, relationships among and within major clades remain unresolved. METHODS: In this study, we evaluate crassiclitellate phylogenetic relationships using 38 new transcriptomes in combination with publicly available transcriptome data. Our data include representatives of nearly all extant earthworm families and a representative of Moniligastridae, another terrestrial annelid group thought to be closely related to Crassiclitellata. We use a series of differentially filtered data matrices and analyses to examine the effects of data partitioning, missing data, compositional and branch-length heterogeneity, and outgroup inclusion. RESULTS AND DISCUSSION: We recover a consistent, strongly supported ingroup topology irrespective of differences in methodology. The topology supports two major earthworm clades, each of which consists of a Northern Hemisphere subclade and a Southern Hemisphere subclade. Divergence time analysis results are concordant with the hypothesis that these north-south splits are the result of the breakup of the supercontinent Pangaea. CONCLUSIONS: These results support several recently proposed revisions to the classical understanding of earthworm phylogeny, reveal two major clades that seem to reflect Pangaean distributions, and raise new questions about earthworm evolutionary relationships.PublishedYe
The NSF/DOE Partnership in Basic Plasma Science & Engineering at 20: Report on the Anniversary Workshop
Get PDFA workshop recognizing the 20th anniversary of the NSF/DOE Partnership in Basic Plasma Science and Engineering was held at NSF on January 9-11, 2017. The workshop demonstrated some of the remarkable research and broader impacts achieved with Partnership support. It provided an opportunity to reflect on the role of the Partnership in supporting plasma research and education, and as a model for interagency cooperation and agency responsiveness to community needs.N