Annals of Spiru Haret University
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THE ELECTROCARDIOGRAPHIC PARAMETERS EVALUATION OF THE SPORT HORSE
A group of 24 sport horses was surveyed for the characteristic elements of the electrocardiograms done before and after a period of 5 months of physical exercises specific to the competition trainings. The sportive performance was positively correlated with the morphology of the ECG waves: the biphasic aspect of wave P, the sS-type aspect of the QRS complex, the monophasic aspect of the T wave. After the training, the average amplitude of P2 biphasic wave increased by 14.7%, the average duration of the QRS complex increased by 16%, of the S wave increased by 21.8% and of the monophasic T wave increased by 22.2%. Key words: sport horse, ECG parameters, adaptative capacit
INFLUENCE OF THE PHYSIOLOGICAL STATE ON THE MINERAL STATUS OF CATTLE HAIR
The mineral status of the cattle hair was assayed in 20 hair samples from pregnant cows and in 20 hair samples from lactating cows from the same farm. Calcium was determined with the complexonometric method; phosphorus was determined with the colorimetric method with ammonium molibdovanadate; magnesium was determined with the colorimetric method with titan yellow. The following values were determined: calcium 177.64± 19.44 mg⁄100g in pregnant cows and 151.86±13.06 mg⁄ 100g in lactating cows; phosphorus 15.92±3.84 mg⁄100g in pregnant cows and 10.70± 4.24 mg⁄100g in lactating cows; magnesium 47.89±6.32 mg⁄100g in pregnant cows and 40.21±2.08 mg⁄100g in lactating cows. The values for the three minerals were significantly lower in the hair samples from lactating cows than in the hair samples from the pregnant cows, which shows that these minerals are used intensely during lactation, sometimes exceeding the homeostatic capacity for retention in the skeleton and, implicitly, in the hair. Key words: cattle, hair, physiological stat
ASPECTS REFERRING TO RECIDIVIST AGGRESSIVE BEHAVIOUR IN ROTTWEILERS
Eleven dogs of the Rottweiler breed were studied in order to notice the evolution of the submission-aggressiveness ambivalent behaviour conditioned by the master (temperament, time allotted to the dog, etc.) and by the environment they live in. All the dogs were trained in similar conditions for six months by specialists. Watchdogs were finally tested and proved to have the qualities for these activities in point of psycho-motive aspects. Eight months after the training came to an end, they checked the behaviour of the dogs and noticed that four dogs had the characteristic features acquired during the training session (watching something and attacking somebody), three had a delated reaction to orders and four had major behavioural deviations, which were conditioned in time, consciously or unconsciously, by their masters’ physiological and nervous features. The behavioural changes were more obvious in the dogs trained for watching a building (three subjects) than in the ones for entertainment (one subject). Taking this into account, the owners or temporary holders of watchdogs must have minimum knowledge of the ambivalent behaviour brought about by the submission-aggressiveness motivational conflict. Key words: Rottweiler, aggressive behaviour
THE BREEDING OF DAIRY SHEEP BY GENETIC MARKERS ASSESTED
The investigation involved 66 Teleorman Black Head Tsigai sheep. The animals were monitored throughout their twelve controls determining the total amount of milk. The genetic markers considered by the investigation were the haemoglobin and transferrin. They were identified two genotypes (HbA/HbB and HbB/HbB) at the haemoglobin locus and eight genotypes at the transferrin locus. The simultaneous analysis of the two studied markers reveals the superiority of the heterozygous sheep HbA HbB/TfMTfE, which displayed the highest productive performance for the studied character. Key words: genetic marker, sheep, haemoblobin, transferri
ASSESSMENT OF BLOOD PRESSURE IN HUMAN-DOG INTERRELATION
Humans with their own dogs, between whom a companion bond had been established, were used as an experimental group (n=5). The control group was represented by dog loving humans with a friendly, but unfamiliar dog, between whom no bond existed (n=5). The cardiovascular response of human respondents (n=17) were also evaluated during quiet reading. The neurochemical and cortisol analyses fall beyond the purpose of this paper. In individuals with their own dogs there was a significant (p<0.0052) percentage of decrease in the MAP, SP, and DP. The MAP of 5 out of 8 individuals decreased by more than 5% during the experiment and remained decreased by more than 5% in 3 individuals, until the end of the experiment. In individuals with unfamiliar dogs there was a non-significant percentage increase in the MAP and DP, and a significant (p=0.00189) percentage of decrease in SP. The MAP for 6 out 8 individuals decreased by more than 5% and remained decreased by more than 5% in 3 individuals, until the end of the experiment.Key words: dogs, blood pressure, interrelation human-do
ASSESSMENT OF PHYSIOLOGICAL RESPONSE IN SPORT HORSES TO TRAINING INDUCED STRESS
Research monitored the induced response of some physiological welfare indicators in sport horses during training. We assessed the stress intensity during training by measuring the variations of heart rate, lactic acid, cortisol and creatinine levels. The horses included in the study were grouped in: untrained (A1 n: 15) and trained (A2 n: 22). Physiological responses differed between the two groups, even if the training programme they underwent was the same. The heart rate and cortisol values increased in untrained horses compared to the trained ones but we recorded an increase of their values in the latter when repeating the exercises during the monitored period (98-120 nmol/l). The plasma lactate levels recorded increased values in the case of the first group (10,2 mmol/l), only until the animals got used to that effort, after which they dropped (9,9 mmol/l). Creatinine concentration increased in the case of trained horses (1,5 mg/dl) compared to the untrained ones (1,2 mg/dl). Horse training may be regarded as stressful under certain circumstances thus leading to a depreciation of their welfare depending on the exercises taken, training intensity and animals’ physical condition. Key words: horses, training, welfar
RESULTS OF INVESTIGATIONS ON THE QUALITY OF FEEDS GIVEN TO DAIRY COWS WITH DIFFERENT STATES OF DISEASE
In a dairy cow farm, during November 2006-March 2007, several morbid states were observed displayed as placenta retention (75-80%), uterus-vaginal secretions and losses by mortality of suckling calves, lower milk yield, etc. Several factors were analyzed to determine the causes of the health problems. Among the analyzed factors, an important role was played by the quality of feeds. The raw ingredients and compound feeds were examined oganoleptically, physico-chemically, mycologically and micotoxicologically in order to determine their involvement in worsening the health state. The results showed significant deviations of the crude protein content, a moderate contamination with toxicogenous fungi and the presence of mycotoxins in excess of the admitted maximal loads. Of the 9 samples examined mycotoxicologically, aflatoxin was determined in 3 samples (compound feed for cows, wheat bran and untoasted sunflower meal) and ochratoxin was determined in 5 samples (compound feed for nursing calves, toasted sunflower meal, compound feed for cows, coarse grinded wheat and corn and untoasted sunflower meal). The presence in feed of aflatoxin and ochratoxin establish the alteration of the health status of the dairy cows.Key words: dairy cows, morbid states, feed quality, mycotoxins, organoleptic and laboratory examinatio
IMMUNOGLOBULIN G RESPONSE OF STREPTOCOCCUS SUIS BACTERIN – VACCINATED PIGS
Thirty SPF pigs known to be free of Streptococcus suis infection were randomly selected from four litters at 10 to 12 days of age and allocated to 20 vaccinates and 10 controls. Each of the vaccinates was injected intramuscularly with a 1 ml dose of a S. suis bacterin at 10-12 days of age, followed by a 2.0 ml dose at 26 days of age. All of the animals were challenged with S. suis 16 days following the second vaccination. The vaccinates were protected and the controls showed clinical signs and necropsy lesions. Western blot analysis identified the special proteins between 35 and 50 kD that were recognized by all sera from pigs vaccinated twice and also by sera from pigs surviving challenge. These 35-50 kD proteins were not recognized by sera from prevaccinates and negative controls. An indirect ELISA using 35-50 kD proteins for coating plates was standardized for the detection of specific antibodies following vaccination and challenge.Key words: Streptococcus suis, immunoglobulin
DIAGNOSIS OF DISMINERALOSES IN CATTLE BY DETERMINATION OF SOME MINERALS IN HAIR. METHODS AND REFERENCES VALUES
In order to elaborate the work technology and establish reference values of some minerals in hair, 50 hair samples were drawn from cows in two farms with best production and reproduction parameters and good sanitary veterinary state. The following parameters were determinated in hair: ash, calcium, phosphorus, magnesium, iron, cupper, manganes, zinc. Analyses were performed by colorimetric and complexometric methods. Zinc was determined by spectrophotometry in atomic absorbtion. The following reference values for minerals in cattle hair were established: ash 1000-2600 mg/100g; calcium 100-230 mg/100g; phosphorus 13-19 mg/100g; magnesium 40-85 mg/100g; iron 30-107 ppm; copper 10-18 ppm; manganes 6-40 ppm; zinc 92-120 ppm. The values of mineral in cattle hair were compared and analyzed with those in blood serum (serum mineral prophile). Key words: dismineraloses, cattle, hair analyses
CORRELATION BETWEEN THE VALUES OF SOME ERYTHROCYTE AND SIDEREMY PARAMETERS IN COWS DURING LATE PREGNANCY AND IN THEIR CALVES
The investigation monitored some haematological and biochemical parameters that characterise iron metabolism in cows during the last month of pregnancy and in their calves during the first three days of life. Haemoglobin and hematocrit were 11.8% and 8.5%, respectively, lower and sideremy was 34.5% higher in the pregnant cows that were cared for improperly, with low protein diets, compared to the cows that received proper diets. The newborn calves from the cows maintained and fed properly had 5.6% and 7.9% values for haemoglobin and, respectively, hematocrit than the calves that resulted from improperly nourished cows. In both categories of calves sideremy had lower values, close to the lower physiological limit, which requires the preventive supplemental with iron of the newborn calves. At birth calves weight was positive correlated with their haemoglobin value and those of their mothers too.Key words: haemoglobin, hematocrit, sideremy, pregnant cows, new born calve