Tokyo University of Agriculture Repository / 東京農業大学リポジトリ
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Harnessing flagellin of Ligilactobacillus agilis as a surface display scaffold for an HIV-1 epitope
Full text linkPossibility and Future Practical Use of Tachibai Irrigation Canal as a Common Capital in the Region
Full text link2014年に世界かんがい施設遺産の認定を受けた立梅用水において、登録までと登録後の地域活動をとりまとめた。多くの地域では登録を受ける前の地域活動は盛り上がるが、登録後の継続的な活動に課題を抱えていた。立梅用水を活用した様々な活動が土台となり、登録後には地域自らが新たな課題を解決する活動を展開していることを示した
A Cross-Sectional Study Based on Forty Systematic Reviews of Foods with Function Claims (FFC) in Japan: Quality Assessment Using AMSTAR 2
Full text linkHow do “biodiversity conservation considerations” change the vegetation management methods?
Full text link本稿では，雑草植生が変わる・変えられるということについて，その事例やメカニズムを紹介した．まず，現に農地管理において生物多様性の保全や創出に大きく舵を切ったヨーロッパの事例を紹介し，日本の水田雑草に含まれる絶滅危惧植物の保全策のあり方との比較を行った．続いてそれらを踏まえ，日本において身近な自然の管理に生物多様性への配慮が必要となった場合を例に，鍵となる考え方や事例を紹介した
Characterization of the NRPS operon homolog for surfactin A and surfactin C synthesis in Bacillus spp.
Full text linkSurfactin is a cyclic lipopeptide produced by Bacillus spp., consisting of a β-hydroxy fatty acid and a heptapeptide synthesized by non-ribosomal peptide synthetases. Surfactin congeners (A, B, and C) differ in amino acid substitutions, with Leu7 in surfactin A replaced by Val in B and Ile in C. Our LC–MS analysis revealed that the elution profiles of surfactin-producing strains could be classified into two distinct patterns under identical culture conditions, corresponding to surfactin A and C production. This suggests that endogenous factors influence surfactin production. Therefore, we aimed to identify the genetic factor that regulates surfactin congener production. The srfA operon for surfactin A biosynthesis in B. subtilis, composed of four open reading frames (ORFs), is srfAABCD. Comparative genomic analysis between the B. subtilis JCM 1465 srfA operon and the TUA12 surfactin biosynthesis genes examined in this study revealed that the operon responsible for surfactin A biosynthesis is distinct, exhibiting 68.7%, 69.2%, 84.7%, and 67.4% homology with the four ORFs, respectively. Similarly, the operon for Ptrs2 surfactin C biosynthesis showed 68.7%, 69.2%, 64.4%, and 67.1% homology. These differences indicate that the identified surfactin A and C biosynthetic operons are novel genetic variants. Further analysis identified the adenylation domain responsible for selecting Ile7 in surfactin C via domain substitution in a surfactin A-producing strain. Average nucleotide identity analysis showed that the surfactin A and C operons were found in B. velezensis and B. amyloliquefaciens, respectively. Our findings suggest that surfactin congener production is species-dependent, with the srf operon specifically distributed in Bacillus spp
