e-Journal Persatuan Dokter Gigi Indonesia (PDGI)
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Clustering Mikrokapsul Silika-Chlorhexidine 2% Menggunakan Kitosan dan Sodium Alginat
Enterococcus faecalis is a leading cause of persistent bacterial infection of the root canal. These bacteria have the ability to invade dentinal tubule penetration making it difficult to eliminate root canal medications. Dentinal tubules are so small between 1-3μm. Microencapsulation of the root canal medicament with a size less than 1μm is expected to be the way out to solve this problem. In this study used sodium silicate as a precursor and chlorohexidine 2% as a root canal the encapsulated drug. The method used is the sol gel Stober process. Clustering using chitosan and alginate materials made to microencapsulation chlorhexidine 2% in higher numbers. The purpose of this study was to obtain silica microcapsules with chlorhexidine 2% of active compound which is less than 1 μm homogeneously with chlorhexidine 2% of the active compound is encapsulated in greater numbers. The results showed that the sequence and composition of the chitosan-alginate affects the formation of clusters, the best results obtained on the composition of the cluster formation of chitosan 2% as much as 6% volume and 0.5 M alginate 2% volume, with an average cluster size of 0.7 μ
Pengaruh Penyikatan Dengan Pasta Gigi Terhadap Kekasaran Permukaan Nano-ionomer Dan Semen Ionomer Kaca Modifikasi Resin
The implementation of nano technology on resin modified glass ionomer cement (RMGIC) results in the latest restorative material which is known as Nano-ionomer. The surface texture of the restoration is one of the most important factors that influences the successful of the material usage. Surface irregularity may cause plaque retention, surface discoloration, and gingival irritation. Objective: To analyze the effect of brushing with toothpastes to the surface roughness of Nano-ionomer and RMGIC. Specimens of Nano-ionomer and RMGIC are brushed with distilled water, anti-cavity toothpaste, and whitening toothpaste (6 Specimens each) for 60 minutes, every 10 minutes the surface roughness are measured with Surface Roughness Tester (Ra). The data are statistically analyzed using Paired-samples t-Test, One-way ANOVA, and Independent-samples t-Test. There is an increase in the surface roughness of both group samples after brushing. As for RMGIC, there is a significant difference between the surface roughness values after brushing with distilled water, anti-cavity toothpaste, and whitening toothpaste, while for Nano-ionomer, the difference is not significant. Once compared, there is a significant difference between the surface roughness increase of RMGIC and Nano-ionomer (p<0.05) which can be seen after brushing for 60 minutes. The increase of surface roughness of RMGIC is significantly higher than Nano-ionomer
Studi pendahuluan silika amorf sekam padi sebagai scaffold sintetis bone graft: efeknya terhadap aktivitas sel osteoblas (In-vitro)
Synthetic bone graft material was developed as a scaffold that serves to template the formation of bone. The ideal scaffold should be biodegradable, osteoindukctive, and osteoconductive. Silica proven to support and promote bone growth. Rice husk contains silica is quite high. The purpose of this study is to analyze the amorphous silica from rice husk as synthetic bone graft material (scaffold), particularly on osteoblast activity in vitro. The research was conducted by isolating silica from rice husk.The results of isolation would be conditioned on the culture medium of primary osteoblasts. Primary osteoblast cultures derived from rat calvaria wistar age of 2 days, growing in Alpha-Modified Eagle Medium (α-MEM) (as a negative control), α-MEM in conditioning with silica 58s (as a positive control) and α-MEM that conditioned with silica from rice husk (as treatment group). Osteoblast activity was observed by analyzing the expression of alkaline phosphatase, using Alkaline phosphatase Kits at days 7 and 14. The results obtained that the rice husk silica were significantly (p <0.05) has the expression of alkaline phosphatase higher than the control group and potitif group. This may imply that the amorphous silica of rice husk affects the increase in osteoblast activity in vitro. It was concluded that rice husk silica increases osteoblast cell activity that is potentially as scaffold for bone graft synthetic material
Rasio Bubuk Polisakarida Daun Cincau Hijau dan Bahan Cetak Alginat Terhadap Recovery From Deformation
Alginate impression material is the most useful material. Nowadays, the material is still be imported that causes the price is relatively expensive and difficult to found in rural area. There were some efforts to modify alginate impression material by mixture Cyclea barbata L. miers leaf polysacharide powder. The impression material must fullfil the criteria of recovery from deformation. Purpose. The aim of this study was to know the ratio of Cyclea barbata L. miers leaf polysacharide powder and alginate impression material that still fullfil the criteria recovery from deformation of impression material according to the ADA. Forty-two samples is divided to six groups of mixture of Cyclea barbata L. miers leaf polysacharide powder in alginate impression material. Group A1 until A6 with the mixture of 0% (control), 40%, 42.5%, 45%, 47.5% and 50%, respectively, Cyclea barbata L. miers leaf polysacharide powder in alginate impression material. Recovery from deformation of the mixture of this impression material measured with recovery from deformation test tool with its 0.01 mm accuracy. There were significant different of Kruskal Wallis test (p<0.05), that interpreted percentage enhancement in Cyclea barbata L. miers leaf polysacharide powder in alginate impression material will increase percentage of recovery from deformation. Increasing of Cyclea barbata L. miers leaf polysacharide powder until 40% into alginate impression material produce recovery from deformation score that can fulfill the ADA criteri
Perbandingan Jumlah Sel Osteoblas pada Penyembuhan Luka Antara Penggunaan Kitosan Gel 1% dan 2%
The use of chitosan in medical fields known as biomaterial nowdays can be used in dental clinical application. Chitosan is obtained by N-acetylating chitin and it is a biodegradable natural biopolymer that can enhance bone healing. The aim of this study was to compare account the rate of osteoblast cells on bone healing process of dental extraction in Rattus norvegicus for 7 and 14 days. Rattus nornegicus strain wistar male, aged 8-16 weeks, divided into 3 treatment groups namely group 1 which given chitosan gel 1%, group II which given chitosan gel 2 % and group III as control which were not given chitosan gel. Chitosan were applied into the socket of dental extraction. Rat was decaputated 7 and 14 days after chitosan application and the jaw in the treated regions and control group were cut for histopatological to observe the number of osteoblast cells. Data were analyzed using t test. The result showed no significant differences in osteoblast cells number between the use chitosan gel 1 and 2 % in 7 and 14 days observation (p<0,05). There was not different number of osteoblast cells with application between chitosan gel 1 % and 2 %. It can be concluded that chitosan gel 1 and 2 % has ability to enhance the bone healing process in post extraction socket of Rattus norvegicus
Penerapan Teknologi Liofilisasi dan Radiasi Sinar γ pada Pembuatan Graf di Indonesia
Penggunaan graf di dunia kedokteran gigi termasuk di Indonesia sudah sejak lama dilakukan, terutama pada prosedur bedah dan perawatan periodontik. Banyak teknologi yang dapat diterapkan dalam pembuatan graf, yang paling populer adalah liofilisasi dan radiasi sinar γ. Liofilisasi atau pengeringan sublimasi adalah pengeringan dengan suhu antara -10oC sampai -40oC, sedangkan radiasi sinar γ adalah proses sterisasi menggunakan sinar γ pada dosis minimum 25 kGy, proses ini merupakan sterilisasi dingin yang tidak mengubah struktur jaringan, tidak meninggalkan residu, dan dapat membunuh mikroorganisme. Pembuatan graf dengan menerapkan kedua teknologi liofilisasi dan radiasi sinar γ ternyata telah dilakukan di Indonesia yaitu di Pusat Aplikasi. Teknologi Isotop dan Radiasi Badan Tenaga Nuklir Nasional (Patir-Batan) dengan prosedur yang merujuk pada prosedur dari International Atomic Energi Agency (IAEA
Aplikasi pasta stroberi sebagai material bleaching terhadap perubahan warna dan kekerasan permukaan enamel
Utilization of natural products are very popular in the community lately as a natural substance that is considered more secure, inexpensive, and easy to obtain than chemical. one of which is to use strawberries as an alternative bleaching materials. The purpose of this research is to determine the optimal time of strawberry paste application as bleaching material that has not lowered the enamel surface hardness. Research method contain six sample groups of post extracted incisive teeth after discolorized with black tea in 12 days. Each sample was soaked in 100% strawberry paste in 5 minutes per 8 hours. Soakings were done in 2, 3, 4, 5 weeks. Then, color discoloration observed using Vita shade guide and the enamel surface hardness measured using Vickers hardness tester. Enamel surface hardness data tabulated and then analyzed using Test of Within Subjects, and if there was a difference it followed by t-Test. Enamel discoloration tested using Friedman Test and if there was a difference it followed by Wilcoxon Test. The result: There was a significant difference in color and enamel microhardness of the samples after being soaked in 100% strawberry paste. So it can be concluted that Strawberry paste is effective as a bleaching material without decreasing the surface microhardness after 2 weeks application. Application of strawberry paste in 3 weeks shall decrease the enamel surface microhardness
Diet Bubuk Cangkang Anadara Granosa dan Susu Kedelai Meningkatkan Kekerasan Permukaan Gigi
Wistar teeth always be growth, but to remodeling needed some minerals and protein to accelerate enamel and dentin to be hardness. Anadara granosa cockle shell showed that it contains a lot of minerals, majority calcium that is important to mineralized of teeth. In the absorption of calcium need amino acid as protein-binding transporter that found in soya milk. The aim of this study was to proved diet combination Anadara granosa cockle shell powder and soya milk increased surface hardness wistar teeth. Twenty eight (28) wistar rats were divided four groups. 1st group was given aquadest, 2nd (Anadara granosa cockle shell powder 0,1368 mg/grBB), 3rd (soya milk 0,015 ml/grBB), 4th (Anadara granosa cockle shell powder 0,1368 mg/grBB and soya milk 0,015 ml/grBB). On 12nd day tooth incisors sample was extracted and done measurement by microhardness vickers. All of datas in this study were analyzed by ANOVA and LSD (p=0,05). This study showed that there was significant difference (p>0.05) between 2nd and 4th group with 1st and 3rd groups so it can be cancluded that the use of diet combination Anadara granosa cockles shell powder and soya milk can increased surface hardness of wistar teeth