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    A075: Endurance Exercise Downregulates SPARC to Improve Age-Related Myocardial Fibrosis in High-Fat Flies

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    Myocardial fibrosis is an important pathological feature of cardiovascular aging, and a high-fat diet (HFD) can accelerate its progression, while SPARC, a remodeling-related protein, may mediate this mechanism. Endurance exercise has been shown to have cardioprotective effects, but its regulatory mechanism on age-related myocardial fibrosis induced by a high-fat diet is still unclear. This study aims to explore whether endurance exercise improves the cardiac fibrosis phenotype in high-fat-fed fruit flies by downregulating SPARC expression and elucidates its underlying molecular mechanisms. Method: Wild-type female fruit flies (n=400) within 12 hours of feathering were selected and randomly divided into four groups (n=100 per group): 10-day-old normal diet control (10D-NC), 10-day-old high-fat diet (10D-HFD), 40-day-old normal diet (40D-NC), and 10-day-old high-fat diet and exercise (10D-HE). The NC group continued to feed normal feed (including yeast, corn, and starch). The HFD group and the HE group received high-fat feed with 30% coconut oil (volume ratio) for 5 days from 5 days old. The HE group performed 2.5 hours of endurance exercise daily from 5 days of age until 10 days of age. 24 hours after the intervention, RT-qPCR was used to detect the expression level of SPARC mRNA in the heart tissue, and cardiac pulsation was recorded by an EM-CCD camera (130 frames/s). The cardiac functional parameters, such as heart rate, cardiac cycle, systolic interval, arrhythmia index, diastolic diameter, and shortening fraction, were quantified in combination with semi-automatic optical analysis software. Compared with the 10D-NC group, the expression of cardiac SPARC in the 10D-HFD group was significantly upregulated (P \u3c 0.01), accompanied by accelerated heart rate (P \u3c 0.001), prolonged cardiac cycle (P \u3c 0.05), increased arrhythmia index (P \u3c 0.001), and decreased shortening score (P \u3c 0.001); compared with the 10D group, the expression of cardiac SPARC in the 40D group was significantly upregulated (P \u3c 0.001), accompanied by accelerated heart rate (P \u3c 0.001), prolonged cardiac cycle (P \u3c 0.001), increased arrhythmia index (P \u3c 0.05), and decreased shortening score (P \u3c 0.05). Compared with the HFD group, SPARC expression in the HE group was significantly downregulated (P \u3c 0.01), heart rate slowing (P \u3c 0.001), shortening of cardiac cycle (P \u3c 0.05), prolonging of systolic interval (P \u3c 0.05), decreased arrhythmia index (P \u3c 0.001), increased diastolic diameter (P \u3c 0.05), and improved shortening score (P \u3c 0.001). There was no statistical difference between the HE groups and the NC group in SPARC expression and various cardiac function indicators (heart rate, ejection fraction, etc.\u3e0.05. Endurance exercise reduces the deposition of cardiac collagen and prevents myocardial fibrosis and dysfunction in Drosophila in HFD

    A144: Research on the Innovative Path of Training International Sports Talents

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    At present, China is rising as an emerging power and facing increasing international challenges. As the foundation of a strong country, sports development is closely related to its comprehensive strength, such as politics and the economy. Increasing the number and quality of international sports organizations is an important condition for promoting the development of China\u27s sports industry and enhancing China\u27s international status and influence. Since then, sports practitioners have undertaken important responsibilities and missions. This study used literature, logical analysis, expert interviews, and case analysis to focus on the dimensions and access system of talent ability training of international sports organizations and comprehensively analyzed the existing pain points of talent training of international sports organizations in China. In the study, the author draws on the three dimensions of the ability and quality of sports talents in international organizations and combines the characteristics of international sports organizations to put forward detailed solutions and ways to ease. 1) Our country\u27s international sports organization talent training program mode is unclear. 2) China\u27s international sports organization talent training joint network has not been formed. 3) The employment system of talent training of international sports organizations is imperfect. 4) China\u27s international sports organization personnel training system guarantee is imperfect. 1) Strengthen the top-level design of personnel training and establish a systematic training mechanism. 2) Establish a joint practice training system of government, association, and university . 3) Expand employment channels and improve welfare benefits. 4) Improve the personnel training system guarantee mechanism

    A262: Effect of Saddle Height on the Biomechanics of Preschool Children\u27s Balance Bike Racing

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    This study aimed to analyze the effects of different saddle heights on the kinematic characteristics and saddle pressure in preschool children and provide recommendations regarding saddle height. Method: Nine preschool children(age:4.6-5.7 years oldwith over two years of balance bike practice experience were filmed using a three-dimensional fixed-point shooting technique to capture their kinematic characteristics at different saddle heights. Fast-Move 1.2.4 software was used to calculate speed, joint angles, angular velocity, and trunk angle. Thin-film resistive pressure sensors were employed to measure saddle pressure 1. The hip joint angle at takeoff was smaller in the high saddle height compared to the original saddle height. The knee joint angular velocity at the original saddle height was higher than that at the low and medium saddle heights, and the ankle joint angular velocity at the original saddle height was higher than that at the medium saddle height. Trunk angles were greater in both the low and original saddle heights compared to the high saddle height. 2. The sensor values in the high saddle height were lower than those in the original saddle height, and the sensor values during riding in the medium saddle height were lower than both the original and low saddle heights. Saddle height has an impact on the kinematic characteristics and saddle pressure in children during competitive balance bike riding. A lower saddle height allows children to have a longer push-off duration, more effective force exertion by joints, and better acceleration. Additionally, children exhibit reduced forward tilt of the trunk and experience lower saddle pressure, resulting in improved safety and comfort

    A193: Taichi Athletes\u27 Superiority in Cross-Modal Tasks: An Explanation from the Perspective of Attribute Amnesia

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    As individuals who have undergone long - term Tai Chi training, Tai Chi athletes may have adaptive changes in brain structure and function, which in turn endow them with unique advantages in cross - auditory - visual tasks. The philosophy of Tai Chi, which emphasizes the combination of internal and external training as well as the unity of mind and body, may assist athletes in maintaining better attention stability and enhancing information integration ability when dealing with complex audio - visual information. Recent research has found that Taichi athletes have superior performance in cross - modal tasks, but the reason is still unknown. This study added a pre - surprise test, surprise test, and post - surprise test to cross - modal rapid serial visual presentation tasks. The pre - surprise test required reporting of the two visual targets T1 and T2, while the surprise and post - surprise tests required additional reporting of auditory stimuli. Method: In our experiment, Taichi athletes and non - athletes were included. A significant interaction in accuracy difference patterns between the two groups under different Lag conditions (F (1, 38) = 6.89, p = 0.013, η² = 0.15). Taichi athletes had higher accuracy in the visual target recognition (T2) task, especially under Lag8 conditions. The Taichi group\u27s accuracy was significantly higher than the non - athlete control group (t (38) = 2.71, p = 0.010, Cohen\u27s d = 0.85). Taichi athletes also exhibited lower attribute amnesia in these tests. In the surprise test, 10 (45.45%) of the Taichi group correctly reported compared to 5 (25%) of the non - athlete control group, with a chi - square test result of χ² (1) = 3.84, p = 0.05. The post - surprise test showed a higher auditory information reporting rate in the Taichi group, but the difference was not significant. Conclusion: Taichi exercise may improve cross - modal tasks and attribute amnesia due to better processing of auditory stimuli

    A012: Relationship Between Physical Activity and Fundamental Movement Skills and Mental Health in Elementary School Children

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    The prevalence of child and adolescent mental health problems has increased in recent years. This study aimed to investigate the association of Chinese elementary school children’s physical activity, fundamental movement skills (FMS), and mental health. Participants were 437 three to six-grade students (196 boys vs. 241 girls; mean age = 10.29 years old; SD = 0.072) at five elementary schools’ children from the province of Hunan in China. The children’s physical activity, FMS, and mental health were measured using the physical activity questionnaire for the older children, the Test of Gross Motor Development-Third Edition (TGMD-3), and the Warwick–Edinburgh Mental Wellbeing Scale, respectively. Data were analyzed with descriptive statistics, independent sample t-tests, Pearson, and linear regression models. The results of the mean and standard deviation showed that the students’ physical activity was generally at a moderate level (M = 2.78, SD = 0.71) in the total group. The boys’ mean scores of physical activities, FMS, and ball skills were higher than the girls ‘(ρ \u3c 0.01). The result of linear regression models showed that physical activity was positively associated with FMS (F (1,433) = 16.006, ρ \u3c 0.001), mental health (F (1,433) = 24.220, ρ \u3c 0.001). Also, FMS were significantly associated with mental health (F (1,433) = 3.2119, ρ \u3c 0.01). Conclusion: Chinese elementary school children’s physical activity is significantly related to both FMS and mental health. Physical activity was a significant contributor predicting FMS in elementary school children. Also, physical activity and FMS are significant predictors of mental health in elementary school children, respectively. Improving the level of physical activity and FMS can effectively promote elementary school children’s mental health

    A264: Factors Influencing Sports Major Students’ Intention to Use Generative AI in Course Learning

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    Generative Artificial Intelligence (GAI) has the potential to enhance both the efficiency and outcomes of course learning for sports major students. However, no existing research has specifically examined the factors that may influence these students\u27 use of GAI in their course learning. The Theory of Planned Behavior (TPB) proposes that attitude (AT), subjective norms (SN), and perceived behavioral control (PBC) are key determinants of behavioral intention. Additionally, perceived ease of use (PEU) and expectation confirmation have been identified as factors that may be associated with intention. Therefore, this study aimed to test an extended TPB model to explore sports major students\u27 intention to apply GAI in their course learning. This study employed a cross-sectional design. A convenience sample of 156 sports major undergraduate students was recruited, with a mean age of 23.52 ± 3.68 years (males = 116, females = 40). Participants completed questionnaires evaluating their AT, SN, PBC, intention, as well as their perceived ease of use and expectation confirmation regarding the use of GAI in their course learning. Structural Equation Modeling (SEM), conducted using SmartPLS software, was utilized to examine an extended TPB model. This model incorporated perceived ease of use and expectation confirmation alongside AT, SN, and PBC to collectively predict intention. The results showed that expectation confirmation (p \u3c 0.01, f² = 0.071) and PEU (p \u3c 0.01, f² = 0.065) significantly and positively predicted intention, along with AT (p \u3c 0.01, f² = 0.059), SN (p \u3c 0.01, f² = 0.061), and PBC (p \u3c 0.01, f² = 0.049). Additionally, AT partially mediated the relationship between PEU and intention (VAF=27.7%). Overall, the combination of PEU, PBC, AT, SN, and expectation confirmation explained a substantial proportion of the variance in intention (R² = 0.501). The extended TPB model is expected to be a valuable framework for explaining sports major students\u27 intention to employ GAI in course learning. Universities may consider integrating GAI-related projects and activities into the sports curriculum to enhance students\u27 practical skills and understanding

    A183: Research on the Path of the Digital Development of Sports Culture

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    The party\u27s 20th report proposed to build a sports power and promote cultural confidence and self-improvement. Campus sports culture needs to borrow digital technology innovation breakthroughs: through intelligent technology to promote the Chinese sports spirit into virtual events, wisdom teaching scenarios, combined with AI and big data implementation physical training and two-way conduction of core values, build digital technology can assign movement practice-cultural gene deepening spiritual identity synergy mechanism, for sports power and cultural confidence provide operational innovation path. To study the path of digital development of sports culture on the comprehensive development of teenagers by means of literature, expert interviews, and research. Practical value: Promote the physical and mental health of teenagers: systematic sports training to promote the all-round development of teenagers: short-term improvement of physical fitness, optimize mental health, form scientific exercise habits in the long term, lay the physical foundation, and the improvement of cultural literacy. Cultivate comprehensive qualities: Through participating in sports activities, students can learn how to cooperate with others and how to stay calm and tough in the competition. These qualities are crucial to their future study and work. Research dilemma: The imbalance of educational resources in urban and rural areas, 23% of rural schools lack standard sports venues (data of the Ministry of Education in 2023); The shortage of professional physical education teachers is about 120,000, and the grassroots training system needs to be improved; The form of sports activities is seriously homogeneous, and the proportion of new media application is less than 35%. Practice path: Resource optimization: establish three-level physical education fund of central + local + society , realize equipment sharing through digital platform; Teacher construction: build online mentor database + AI teaching plan system , carry out digital certification of teachers\u27 ability; form innovation: develop physical education space, conduct simulation training through VR, monitor real-time physical data through sports bracelet. The development of sports culture in primary and secondary schools needs to focus on building the organic integration of the digital support system and traditional education. Through a series of measures such as building the intelligent management platform of sports resources, the feelings of family and country are cultivated with the sports culture gene, and the sports spirit and innovative character of young people are cultivated with the help of digital empowerment, so as to inject source impetus into the construction of a strong sports country and serve the strategic needs of national rejuvenation

    A102: Differential Modulation of Sensorimotor Network Between Temporal Interference and High-Definition Direct Current Stimulation

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    Temporal interference stimulation (TIS) is a promising non-invasive deep brain stimulation technique that has the potential to enhance motor function. However, potential efficacy in modulating brain networks remains largely unexplored. The present study aimed to evaluate the differential impact on brain networks of TIS and high-definition transcranial direct current stimulation (HD-tDCS) on resting-state functional connectivity (FC) and topological properties of brain networks, particularly the Sensorimotor Network (SMN). Method: In a randomized, double-blind, crossover design, 40 healthy adults received both TIS (20 Hz beat frequency, 2 mA) and HD-tDCS (2 mA) targeting the left primary motor cortex (M1). Resting-state functional magnetic resonance imaging (Rs-fMRI) data were collected pre-, during, and post-stimulation. Brain networks were constructed using the Dosenbach atlas (142 ROIs, excluding the cerebellum) and the Yeo seven-network parcellation, focusing on 29 ROIs in the SMN. Network analysis included FC calculations using Pearson correlations and graph-theoretical measures of global and local efficiency. Two-way repeated measures analysis of variance (ANOVA) evaluates the effects of stimulation on network connectivity properties. (1) Both stimulation methods enhanced SMN connectivity, with TIS showing greater efficacy than HD-tDCS (FC increase, 31.25% vs. 22.58%). (2) TIS significantly enhanced SMN global and local efficiencies by 15.44% and 18.85%, respectively; HD-tDCS increased these measures by 11.97% and 12.5%, respectively (all p \u3c 0.05). (3) While both methods enhanced SMN within-network connections, TIS strengthened the Visual Network (VN), Dorsal Attention Network (DAN), and Frontoparietal Network (FPN) within-network connections and multiple between-network connections, whereas HD-tDCS only improved connections between SMN-DAN and SMN-Default Mode Network (DMN). Both TIS and HD-tDCS enhanced SMN functional connectivity, with the TIS demonstrating efficacy. TIS exhibited broader effects, improving both network efficiency and extensive within- and between-network connections, whereas HD-tDCS had a limited impact on the selection of network connections. These findings establish TIS as a potentially more effective neuromodulation technique for enhancing brain network properties than HD-tDCS

    A231: Effects of a Six-Week Pre-Competition Program on Blood Biomarkers in Female Adolescent Rowers

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    Rowing is a high-intensity cyclic endurance sport that demands precise regulation of training loads to optimize performance. Adolescent athletes undergoing rapid growth and endocrine changes may respond differently to training than adults. However, most current research focuses on adult rowers, with limited evidence available for adolescent females. This study aimed to evaluate the effects of a six-week pre-competition training program on the blood biochemical markers of adolescent female rowers in order to inform age-appropriate training strategies. Twenty adolescent female rowers (age: 13.9 ± 0.7 years; height: 169.0 ± 4.85 cm; weight: 51.2 ± 11.6 kg) were recruited. The study adopted a pre-post-experimental design over six weeks. All participants followed a standardized pre-competition training regimen. Blood samples were collected in a fasted state before and after the intervention. The following markers were analyzed: Hematological: red blood cell count (RBC), hemoglobin (HGB), hematocrit (HCT), and mean corpuscular volume (MCV). Endocrine: testosterone (T), cortisol (C), and testosterone-to-cortisol ratio (T/C). Metabolic: blood urea nitrogen (BUN) and creatine kinase (CK). Immune: white blood cell count (WBC), immunoglobulin G (IgG), immunoglobulin M (IgM), and immunoglobulin A (IgA). Biochemical parameters were assessed using standard laboratory assays. Paired-sample t-tests were performed using SPSS 27.0 to evaluate pre-post changes, with significance set at P \u3c 0.05. Post-training, all indicators remained within clinical norms. RBC and MCV significantly increased (P \u3c 0.05), while HGB and HCT showed non-significant upward trends. Testosterone and the T/C ratio increased significantly (P \u3c 0.05), whereas cortisol showed a slight, non-significant increase. BUN decreased, and CK increased without reaching significance. WBC slightly decreased, and immunoglobulin levels remained stable. The six-week pre-competition training led to beneficial adaptations in oxygen transport capacity and endocrine response, as evidenced by increased RBC, MCV, and testosterone. Metabolic and immune markers remained unchanged, suggesting that the training load was appropriate and did not induce excessive physiological stress. These results align with prior findings in adolescent athletes but provide new evidence specific to female rowers. Limitations include a small sample size and a short observation period. Future studies should explore long-term effects and larger cohorts. This research offers valuable guidance for designing scientifically grounded training and monitoring protocols in youth rowing

    A039: Determining the Type of Aerobic/Anaerobic Metabolism During Exercise in Drosophila by Incremental Loading Exercise

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    Existing Drosophila exercise models only distinguish between regular and endurance exercise based on behavioral traits and have not yet established an exercise physiology classification system based on metabolic traits, which restricts the depth of application of this model organism in exercise physiology research. This study aims to establish an aerobic/anaerobic metabolic model of Drosophila by constructing an incremental load exercise model. Method: A 7-day-old wild-type Drosophila was used as the study subject for the exercise intervention using a Drosophila exercise device. Six exercise intensity gradients were used in this study: 0.31 rev/s (E1), 0.45 rev/s (E2), 0.59 rev/s (E3), 0.71 rev/s (E4), 0.83 rev/s (E5), and 0.91 rev/s (E6). Drosophila in all exercise groups were subjected to a single acute exercise session of 2.5 h. No exercise intervention was performed in the control group. At the end of the exercise, the expression levels of anaerobic metabolizing enzymes (Hex-A, Hex-C, Pfk, Pyk, Ldh) and aerobic metabolizing enzymes (dPdh-A, dPdh-B, Idh, Nc73EF, whd) in Drosophila were detected by RT-qPCR. Meanwhile, the type of aerobic and anaerobic metabolism during Drosophila exercise was comprehensively analyzed by measuring Drosophila blood lactate concentration, respiratory quotient, mitochondrial function, and alginate concentration. 1) Anaerobic metabolism key enzyme activity showed a peak response at E5 loading intensity, and aerobic metabolism marker enzyme activity reached the activation threshold at E2 intensity; 2) blood lactate kinetic curve showed that the overall concentration was significantly higher than that of the control group (P \u3c 0.05), and a significant inflection point at E5 intensity; 3) a significant increase in the respiratory quotient occurred when the loading intensity was elevated to E5 and E6 (P \u3c 0.01); 4) Trehalose concentration decreased dose-dependently with loading intensity; 5) Mitochondrial maximal respiratory capacity was enhanced with exercise loading, and a significant elevation of E5 intensity occurred (P \u3c 0.05), and E6 intensity entered a plateau; 6) The metabolic effects produced by E5 and E2 loading interventions were homologous to the characteristics of human aerobic/anaerobic exercise benefit. Drosophila showed a dominant anaerobic metabolic phenotype at 0.83 rev/s (E5) loading intensity, while 0.45 rev/s (E2) intensity showed a typical aerobic metabolic pattern

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