Alberto Sols Biomedical Research Institute
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Game on: curbing impulse buying and returns in apparel e-tailers
No full textThis study explores the effectiveness of gamified strategies in addressing impulsive e-buying behaviors and mitigating subsequent product returns. Impulse buying in apparel e-tailers is a prevalent phenomenon with significant implications for both consumers and businesses. While impulse purchases contribute to e-tailers' sales, they also result in undesired consequences such as regret, dissatisfaction, and high return rates. This research investigates the potential of gamification, specifically distraction and substitution strategies, in counteracting impulsive online purchase decisions. Through online experimental and field studies involving participants from the United States and a European apparel e-tailer, respectively, we examine the impact of gamified interventions on impulse buying behavior and product return rates. The findings indicate that gamified distraction strategies are more effective than substitution strategies in reversing impulse e-buying behavior. Additionally, the field experiment demonstrates a significant reduction in product return rates among customers exposed to distraction interventions. These results highlight the potential of gamification as a nonpunitive approach to intervene in the e-decision-making process of consumers, offering practical implications for e-tailers seeking to minimize returns and enhance customer satisfactio
Double catalytic activity unveiled: Synthesis, characterization, and catalytic applications of iridium complexes in transfer hydrogenation and photomediated transformations
No full textIridium complexes have been demonstrated to be highly active catalysts for a wide variety of transformations. Their unique photophysical and photochemical properties render them as one of the most established photocatalysts. Moreover, iridium complexes are widely acknowledged for their efficiency in transfer hydrogenation reactions. However, the development of iridium complexes able to promote both traditional organometallic catalysis and photocatalysis is scarce. Thus, the design of iridium-based catalysts is still an active area of research. In this context, we targeted the synthesis of a family of Ir-Cp* systems to explore their (photo)catalytic applications. Here, we describe the synthesis, structural characterization, and photophysical properties of iridium complexes of formula [IrCp*Cl(N^O)]. These complexes have been applied with a double catalytic function, in transfer hydrogenation for carbonyl reduction and in different photomediated transformationsFinancial support was provided by the Spanish Government (PID2021-122299NB-100, TED2021-130470B-100, and TED2021-129999B-C32), “Comunidad de Madrid”, European Structural Funds (S2018/NMT-4367), and “Proyectos sinérgicos I+D” (Y2020/NMT6469). A.C. thanks the Comunidad de Madrid for “Proyecto I+D de la CAM para Jóvenes Doctores de la UAM” (SI3/PJI/2021-00520) and the Agencia Estatal de Investigación for “Consolidación Investigadora 2022” project CN2022-135687 funded by the MICIU/AEI/10.13039/ 501100011033 and by the European Union NextGenerationEU/PRT
The influence of knowing the source of information on readers’ perceptions. An exploratory study on twitter
No full textDisinformation, fake news or misinformation are well-known problems in social networks. Nevertheless, the readers play an active role-part because they consume and propagate such information. In this paper, we wonder how the readers perceive the information by identifying the source of information. The work presented in this paper details the exploratory study we conducted to check whether knowing the source of information influence people’s decision when identifying types of information, using a classification of information based on argumentation. Thus, we analysed the information that is spread on Twitter, a digital social network that works as a micro-blog where users can post short open messages in real-time, which we know as tweets, and researchers can access information about them. The results obtained through the application of statistical tests and their contrast with graphic methods show that the decision of people that know the source does not influence when identifying as “fact or data”, “opinion or judgment” and “counterargument”. However, the opposite occurs for the type of information labelled as “argument”. In addition, the results reveal that opinions spread more than facts on TwitterThis research work has been co-funded by the Spanish National
Research Agency (Agencia Estatal de Investigación) through the project Indigo!
(PID2019-105951RB-I00), and the Regional Government of Madrid (Comunidad de
Madrid) through the e- Madrid-CM project (P2018/TCS-4307). The e-Madrid-CM project
is also supported by Structural Funds (ESF and ERDF
Optimización y alternativas al sistema de amplificación de DNA TruePrime®
No full textTesis Doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de Lectura: 13-07-2023Esta tesis tiene embargado el acceso al texto completo hasta el 13-01-2025The development of several isothermal DNA amplification methods has brought
improvements in terms of simplicity, sensitivity, or yields. Many of these have become
fundamental tools in genomic research, clinical diagnostics, and forensic studies. Moreover,
with the recent approval of gene therapy products and nucleic acid vaccines, one of the main
challenges being addressed is the increased demand for large-scale production and
manufacturing of highly specific genetic material. This leaves room for enzymatically produced
clinical grade synthetic DNA.
TruePrimeÒ is an isothermal DNA amplification method which does not require external
primers (random or specific) as it combines two DNA-synthesizing enzymes (Picher et al.
2016). A DNA primase enzyme from Thermus thermophilus named PrimPol (TthPrimPol), uses
dNTPs as substrates to produce DNA primers at different initiation sites of the given template.
These DNA primers are then efficiently elongated by the high-fidelity and highly-processive
DNA polymerase of bacteriophage Phi29 (Phi29 DNApol). The outstanding stranddisplacement
capacity of Phi29 DNApol allows the continuous exposure of single-stranded
DNA (ssDNA) for new priming events, thus resulting on an exponential cascade of multiple
displacement amplification (MDA).
We have identified and characterised a new PrimPol from T. thermophilus SG0.5JP17-
16 strain, TthPrimPol2, which is endowed with DNA primase and DNA polymerase activities,
triggered by both magnesium or manganese ions. Its enzymatic activities were superior to
those of TthPrimPol and although it has a similar preference for template initiation sites, it
processively synthesizes longer DNA primers, endures higher temperatures, and most
importantly, has a stronger ssDNA binding affinity. Its combination with Phi29 DNApol enabled
the detection and amplification of lower input DNA than TruePrimeÒ, valuable for its application
when DNA is found in limiting amounts, such as in liquid biopsies.
Furthermore, we have assessed the ability of a new B-family DNA polymerase
endowed with de novo DNA synthesis capacity, piPolB, to perform DNA amplification as a
single enzyme. No DNA amplification was detectable with piPolB alone, but its combination
with Phi29 DNApol did yield competitive amounts of DNA, suggesting piPolB can initiate DNA
primers synthesis but requires Phi29 DNApol for proficient elongation. Detailed fidelity analysis
of the amplified material showed low error rates although piPolB-primed reactions contained a
higher number of indels than TruePrimeÒ, especially at homopolymeric repeats. Better
genomic coverage values were obtained for lower piPolB concentrations, suggesting high
amounts of piPolB could interfere with the reaction. Conversely, the presence of piPolB in the
reaction, a TLS polymerase enzyme, could benefit the amplification of challenging or damaged
templates, where extreme accuracy of the resulting material is not the priorityThis thesis has been
financed through the grants for Industrial Doctorates (Ref. IND2018/BMD-9984, Dirección
General de Investigación e Innovación, Consejería de Educación e Investigación de la
Comunidad de Madrid
Theoretical study of aromatic single-molecule junctions with DFT-based methods
No full textTesis Doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Química. Fecha de Lectura: 11-07-2023Esta tesis tiene embargado el acceso al texto completo hasta el 11-01-202
The reserved manuscript indexes of the Library of the Ducal House of Medinaceli (1759)
No full textEn este trabajo estudiamos el fondo manuscrito de la biblioteca ducal de Medinaceli. En primer lugar, ofrecemos un panorama general de la colección reunida en el siglo XVIII en las salas de reservados de la biblioteca ducal. Para ello, utilizamos cuatro de sus catálogos, hoy conservados en la biblioteca de la Fundación Bartolomé March (Palma de Mallorca). En segundo lugar, detallamos la metodología que seguimos en la reconstrucción de esta parte de la colección de Medinaceli, fijando la procedencia e identificando y localizando algunos de los ejemplares conservadosThis paper studies the manuscripts of the Ducal Library of Medinaceli. First of all,
we give a general vision of the collection, put together in the 18th Century in certain
special rooms in the Library. To achieve this, we make use of its catalogues, kept today
in the Library of Fundación Bartolomé March, (in Palma de Mallorca). Secondly, we
detail the methodology followed in the reconstruction of this part of the collection, determining
the origin of the volumes and identifying and locating some of the currently
existing copiesEste trabajo se inscribe, por un lado, en el proyecto de investigación pid2020-117488gb-I00, financiado
por el Ministerio de Ciencia e Innovación del Gobierno de España, en el marco del proyecto
de i+d+i, convocatoria 2020, del programa estatal de Generación del conocimiento y fortalecimiento
científico y tecnológico, desde el 01/09/2021 hasta el 31/08/2025, y, por otro, en los proyectos de
investigación Les idées de la poésie en Espagne entre Baroque et Néoclassicisme (1650-1750) y République
des Lettres et res litteraria hispaniques dans l’Europe du long temps des novateurs (1650-1750), financiados
por el Centre National de la Recherche Scientifique (umr 5136 framespa, Université de Toulouse-Jean
Jaurès, Francia), desde el 01/01/2021 hasta el 31/12/202
New materials based on Covalent Organic Frameworks for water treatment
No full textTesis Doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Química Inorgánica. Fecha de Lectura: 19-04-2024Esta tesis tiene embargado el acceso al texto completo hasta el 19-10-202
Estudio del potencial de la edición génica mediante la tecnología CRISPR/Cas9 para el tratamiento de la distrofia muscular congénita asociada a LMNA
No full textTesis Doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Medicina, Departamento de Bioquímica. Fecha de Lectura: 21-05-2024Esta tesis tiene embargado el acceso al texto completo hasta el 21-11-2025La distrofia muscular congénita asociada a LMNA (L-CMD) es una enfermedad genética rara de aparición temprana, caracterizada por una debilidad muscular progresiva, acompañada de miocardiopatía dilatada e insuficiencia respiratoria. Su origen radica en mutaciones del gen LMNA, el cual codifica las proteínas laminares A y C, componentes esenciales de la lámina nuclear. La ausencia de tratamientos curativos hace que el objetivo de este estudio sea explorar nuevas aproximaciones terapéuticas basadas en la edición génica. Para ello, se utilizó el sistema CRISPR/Cas9 junto con guías de ARN específicas para eliminar la mutación Lmna c.745C>T (p.R249W), buscando revertir el fenotipo patológico en diversos modelos celulares y en el modelo de ratón LmnaR249W. Además, se empleó esta tecnología para desarrollar un modelo de ratón con hemicigosis de Lmna, permitiendo estudiar el impacto de esta condición genética en la supervivencia y la fisiología cardiaca.
Los resultados obtenidos en modelos celulares portadores de la mutación R249W demostraron una marcada especificidad de los complejos Cas9/sg745T hacia el alelo mutante. Esta propiedad varió en función de la concentración de los componentes CRISPR, observándose una pérdida de la especificidad con el incremento de la dosis. La aplicación de complejos Cas9/sg745T-m en embriones permitió la creación del ratón Lmnatru. Este modelo hemicigótico para Lmna muestra una reducción significativa de los niveles de lámina A/C. Esta hemicigosis no afectó a la viabilidad, ni propició el desarrollo de miocardiopatía dilatada en los animales Lmna+/tru, lo cual nos condujo a explorar in vivo el potencial terapéutico de una terapia CRISPR orientada a la destrucción del alelo mutante Lmna c.745C>T. Esta estrategia, a pesar de estar asociada a una actividad CRISPR reducida, supuso un aumento del 10% en la supervivencia de los ratones homocigotos R249W. En contraste, la misma actividad CRISPR mejoró la patología cardiaca desarrollada por los animales Lmna+/R249W, extendiendo significativamente su supervivencia media. La terapia CRISPR también fue analizada en combinación con la sobreexpresión de lámina WT exógena. Al administrarse en animales LmnaR249W/R249W, la sobreexpresión de lámina WT, sola o en combinación con los complejos Cas9/sg745T-m, provocó una mejora del fenotipo patológico y un aumento significativo en la supervivencia. Sin embargo, tuvo un impacto negativo en los ratones heterocigotos R249W.
Con este trabajo se demuestra la variabilidad existente en cuanto a la eficiencia de distintas terapias génicas para abordar la L-CMD. Nuestros resultados son especialmente prometedores en el caso de la estrategia encaminada a la destrucción del alelo mutante R249W, y constituyen la primera validación terapéutica basada en la edición génica mediada por CRISPR/Cas9 para el tratamiento de la L-CM
Autarky in Franco's Spain: The costs of a closed economy
Get PDFBetween the 1940s and 1970s, Spain used a variety of economic policies that hindered international trade. Because the mix of tariffs, quotas, administrative barriers, and exchange rate regimes varied greatly over time, the quantification of the effect of the various trade policies on international trade in this period is particularly elusive. In this paper, we use historical bilateral trade flows and a structural gravity model to quantify the evolution of Spain's border thickness, a summary measure of its barriers to international trade. We find that Spain's borders in the period 1948–75 were thicker than those of any other country in Western Europe, even after the liberalization of trade that started in 1959. These comparatively higher impediments to international trade implied substantial negative effects on consumer welfare. We estimate that accumulated welfare costs over the period 1948–75 exceed 20 per cent of a year's total consumptionIliana Reggio acknowledges financial support by theSpanish Ministerio de Ciencia, Innovación y Universidades (Grant PID2019-107916GB-I00). Theviews expressed in this paper are those of the authors and do not therefore necessarily reflect thoseof the Banco de España or the Eurosyste
Francisco de Paula Canalejas y Casas (1834-1883): un «notario» del krausismo
No full textTesis Doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Filosofía y Letras, Departamento de Antropología Social y Pensamiento Filosófico Español. Fecha de Lectura: 24-06-2024Esta tesis tiene embargado el acceso al texto completo hasta el 24-12-2025El estudio de la vida y la obra de Francisco de Paula Canalejas constituía un reto pendiente
tanto a nivel epistemológico y filosófico por la expectativa de las aportaciones que podía
ofrecer a la comprensión del krausismo español, en particular, como del pensamiento
filosófico y la realidad socio-politica y cultural española del siglo XIX, en general. Esta
impresión había sido manifestada por diversos críticos desde hace tiempo pero la
investigación sobre Canalejas no terminaba de concretarse en un trabajo extenso, especifico y
riguroso.
Los principales resultados de la investigación se han concretado en una biografía
completa, revisada y documentada en base a datos historiográficos y hemerográficos; un
resumen detallado de la totalidad de sus escritos, tantos libros como artículos; un análisis
crítico de su pensamiento aplicado a las diferentes facetas de su vida pública e intelectual
(derecho, estética y literatura, prensa, política y filosofía); y, por último, una serie de
conclusiones y desafíos referidos a la comprensión de la aportación de Canalejas y su lugar en
el denominado krausismo español.
La tesis está dividida en ocho apartados que pueden englobarse en 4 bloques. Un primer
bloque está dedicado a la información más técnica, bibliográfica y documental de la
investigación.
El siguiente bloque se identifica con el capítulo dedicado a la biografía de Francisco de
Paula Canalejas. Para ello se han subdividido el capítulo en apartados específicos y detallados
sobre seis etapas reseñables en su biografía.
El tercer bloque responde al resumen y análisis de la obra del autor, tanto en forma de
libro como de artículos y clasificados cronológicamente y por temáticas.
A modo de síntesis, un último bloque dividido en dos partes ofrece una valoración más
específica de la aportación de Canalejas en los principales ámbitos de su pensamiento
(jurídico, estético, literario, prensa, político y filosófico) y de su vida pública y una conclusión
general que recogiese un balance de su figura y su impronta personal y filosóficaThe study of the life and work of Francisco de Paula Canalejas constituted a pending
challenge both at the epistemological and philosophical level due to the expectation of the
contributions he could offer to the understanding of Spanish Krausism, in particular, and of
philosophical thought and the Spanish socio-political and cultural reality of the 19th century,
in general. This impression had been expressed by several critics for a long time, but the
research on Canalejas had not yet materialized in an extensive, specific and rigorous work.
The main results of the research have settle on a complete biography, revised and
documented based on historiographic and newspaper data; a detailed summary of all his
writings, both books and articles; a critical analysis of his thought applied to the different
facets of his public and intellectual life (law, aesthetics and literature, press, politics and
philosophy); and, finally, a series of conclusions and challenges related to the understanding
of Canalejas' contribution and its place in the so-called Spanish krausism.
The thesis is divided into eight sections that can be grouped into four blocks. The first
block is dedicated to the more technical, bibliographical and documentary information of the
research.
The next block is identified with the chapter dedicated to the biography of Francisco de
Paula Canalejas. For this purpose, the chapter has been subdivided into specific and detailed
sections on six notable stages in his biography.
The third section is a summary and analysis of the author's work, both in the form of
books and articles, classified by subject.and chronologically.
As a synthesis, a last block divided into two parts offers a more specific evaluation of the
contribution of Canalejas in the main areas of his thought (legal, aesthetic, literary, press,
political and philosophical) and of his public life, and a general conclusion that includes a
balance of his figure and also his personal and philosophical imprin