University of Castilla-La Mancha
Universidad de Castilla-La Mancha: Repositorio Universitario Institucional de Recursos Abiertos (RUIdeRA)Not a member yet
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Automatización y control de despliegue de infraestructuras.
Get PDFEn este Trabajo Fin de Grado, se diseña e implementa un workflow para la empresa, que
utiliza una serie de metodologías y herramientas basadas en la cultura DevOps. Con este
objetivo se desarrollan un conjunto de scripts y procedimientos propios con el fin de estandarizar
una nueva forma de trabajo, además ayudará a mejorar los tiempos del ciclo de
vida de los proyectos. Entre las herramientas DevOps sobre las que me he apoyado para el
proyecto se encuentran Docker, Ansible, Jenkins, GitLab y ElasticSearch, entre otras.
Las herramientas desarrolladas integrarán la automatización y virtualización al mundo de
productos Oracle. Evitando pérdidas de tiempo y fallos humanos en los despliegues de infraestructuras,
donde cualquier entorno nuevo será creado y gestionado por estas herramientas,
unificando así, tanto entornos de desarrollo como despliegues en máquinas reales para clientes.
Fundamentalmente, el proyecto ha consistido en la creación de una serie de scripts de
instalación que puedan ser integrados dentro de un flujo de integración continua, consiguiendo
un sistema de despliegue automatizado.
La incorporación de este nuevo flujo de trabajo, supondrá un antes y un después para la
empresa, modificando la mentalidad de trabajo a una gran escala. El objetivo es conseguir un
control total sobre los despliegues de infraestructuras de modo que no se tengan problemas
de configuraciones cambiantes entre máquinas de un entorno y todos los equipos trabajen en
sistemas uniformes con posibilidad de adaptación.In this Final of Degree Project, a workflow is designed for the company, which uses a series
of methodologies and tools based on the DevOps culture. The purpose of this combination of
tools and procedures aims to standardize the way of current work, in addition to accelerating
the life cycles of the projects. Among the tools used for the project are Docker, Ansible,
Jenkins, GitLab and ElasticSearch among other.
The tools developed will integrate automation and virtualization to the world of Oracle
products. Avoiding wasted time and human failures in the deployments of infrastructures
where any new environment will be created and managed by these tools, unifying both development
environments and deployments in real machines for customers.
The incorporation of this new workflow will mean a step forward for the company, modifying
the work mentality on a larger scale than a simple application of procedures. The
objective is to achieve total control over infrastructure deployments structures so that do not
have problems of changing configurations between machines of an environment and all the
teams work in uniform systems with the possibility of adapting
Linear and Nonlinear Features and Machine Learning for Wind Turbine Blade Ice Detection and Diagnosis
Get PDFThe mass of ice on wind turbines blades is one of the main problems that energy companies have in cold climates. This paper presents a novel approach to detect and classify ice thickness based on pattern recognition through guided ultrasonic waves and Machine Learning. To successfully achieve a supervised classification, it is necessary to employ a method that allows the correct extraction and selection of features of the ultrasonic signal. The main novelty in this work is that the approach considers four feature extraction methods to validate the results, grouped by linear (AutoRegressive (AR) and Principal Component Analysis) and nonlinear (nonlinear-AR eXogenous and Hierarchical Non-Linear Principal Component Analysis), and feature selection is done by Neighbourhood Component Analysis. A supervised classification was performed through Machine Learning with twenty classifiers such as Decision tree, Discriminant Analysis. Support Vector Machines, K-Nearest Neighbours, and Ensemble Classifiers. Finally, an evaluation of the classifiers was done in single frequency and multi-frequency modes, obtaining accurate results
Tick-host-pathogen interactons and vaccine development for the control of tick-borne diseases
No full textTick-borne diseases have a negative impact on human and animal health worldwide. Ticks are the most important vectors of pathogens that cause different diseases in humans, domestic and wild animals. Traditional tick control methods are primarily based on the use of chemical acaricides, which can result in the selection of resistant ticks, environmental pollution, and contamination of milk and meat products with residues affecting in particular the cattle industry.
Chapter I: General introduction
Content: Chapter I is a review paper [de la Fuente, J., and Contreras, M. (2015). Tick vaccines: current status and future directions. Expert. Rev. Vaccines. 14, 1367-1376] that constitute an introduction for this thesis by reviewing the importance of tick vaccines as an alternative environmentally sound approach for the control of tick infestations and pathogen infection and transmission. This chapter also describes critical issues, and recommendations for future directions towards developing improved and effective tick vaccines. The results with commercial vaccines for the control of cattle tick infestations support the efficacy of tick vaccines under field conditions. Future strategies include the vaccinomics pipeline developed by our group for the identification and characterization of tick protective antigens. Vaccinomics is a holistic approach based on the use of genome-scale or omics technologies integrated in a systems biology approach to characterize tick-host-pathogen interactions for the development of next-generation vaccines. In vaccinomics, the integration of “omics” technologies can improve the probability of identifying new candidate protective antigens, therefore advancing the development of effective vaccines. Furthermore, we propose that the combination of tick-derived and pathogen-derived protective antigens could result in more effective vaccines for the prevention and control of tick-borne diseases.
Chapter II: Vaccinomics for the identification and characterization of candidate protective antigens
Content: In chapter II, the intracellular bacterium, Anaplasma phagocytophilum, which causes human and animal granulocytic anaplasmosis, and its tick vectors, Ixodes scapularis and I. ricinus are used as models for the identification of tick-derived and pathogen-derived candidate protective antigens using a vaccinomics approach. The sequence, assembly and annotation of the I. scapularis genome were recently released, and transcriptomics studies in I. ricinus suggest that these tick species are genetically closely related [Genomic Resources Development Consortium, Contreras, M., de la Fuente, J., Estrada-Peña, A., Grubhoffer, L., and Tobes, R. (2014). Transcriptome sequence divergence between Lyme disease tick vectors, Ixodes scapularis and Ixodes ricinus. Genomic Resources Notes accepted 1 April 2014 – 31 May 2014. Mol. Ecol. Resour. 14: 1095]. The vaccinomics pipeline was developed based on quantitative transcriptomics and proteomics data from uninfected and A. phagocytophilum-infected I. scapularis nymphs, adult female midguts and salivary glands [Contreras, M., Villar, M., Alberdi, P., and de la Fuente, J. (2016). Vaccinomics approach to tick vaccine development. Methods. Mol. Biol. 1404, 275-286]. This approach resulted in the identification of the candidate tick protective antigens, lipocalin (ISCW005600) and lectin pathway inhibitor (AAY66632) for the control of vector infestations and A. phagocytophilum infection [Contreras, M., Alberdi, P., Fernández de Mera, I.G., Krull, C., Nijhof, A., Villar, M., de la Fuente, J. (2017). Vaccinomics approach to the identification of candidate protective antigens for the control of vector infestations and pathogen infection. Front. Cell. Infect. Microbiol., 7:360. doi:10.3389/fcimb.2017.00360]. Additionally, the characterization of protein-protein interactions at the host-pathogen interface are characterized in order to discover and design new pathogen-derived protective antigens [Contreras, M., Alberdi, P., Mateos-Hernández, L., Fernández de Mera, I.G., García-Pérez, A.L., Vancová, M., et al. (2017). Anaplasma phagocytophilum MSP4 and HSP70 proteins are involved in interactions with host cells during pathogen infection. Front. Cell. Infect. Microbiol. 7: 307]. The results show that A. phagocytophilum MSP4-HSP70 are involved in interaction and infection of host cells, but were only partially protective against pathogen infection in sheep. However, these antigens may constitute candidate protective antigens for the development of vaccines against tick borne diseases when used in combination with other antigens.
Chapter III: Vaccination trails with tick protective antigens
Chapter III describes vaccination trials that were conducted to evaluate the protective capacity of tick protective antigens for the control of tick infestations. The efficacy of the Q38 chimera, containing Subolesin (SUB) and Akirin (AKR) conserved protective epitopes, was evaluated on I. ricinus and Dermacentor reticulatus. It was demonstrated that Q38 vaccination had an efficacy of 99.9% and 46.4% on the control of I. ricinus and D. reticulatus larvae, respectively [Contreras, M., de la Fuente, J. (2016). Control of Ixodes ricinus and Dermacentor reticulatus tick infestations in rabbits vaccinated with the Q38 Subolesin/Akirin chimera. Vaccine 34: 3010-3013]. In a second trial, vaccination with the I. ricinus aquaporin (IrAQP) and a tick aquaporin conserved region (CoAQP) on I. ricinus larvae infestations had an efficacy of 32% and 80%, respectively [Contreras, M., de la Fuente, J. (2017). Control of infestations by Ixodes ricinus tick larvae in rabbits vaccinated with aquaporin recombinant antigens. Vaccine 35: 1323-1328]. Finally, the characterization, composition and immunogenicity of the membrane-bound SUB-Anaplasma marginale MSP1a chimeric antigen is presented [Contreras, M., Moreno-Cid, J.A., Domingos, A., Canales, M., Díez-Delgado, I., Pérez de la Lastra, J.M., Sánchez, E., Merino, O., López Zavala, R., Ayllón, N., Boadella, M., Villar, M., Gortázar, C., de la Fuente, J. (2015). Bacterial membranes enhance the immunogenicity and protective capacity of the surface exposed tick Subolesin-Anaplasma marginale MSP1a chimeric antigen. Ticks and Tick-Borne Diseases 6: 820-828]. The SUB-MSP1a chimeric antigen was produced in Escherichia coli as a membrane-bound and exposed protein and used to protect vaccinated cattle against tick infestations. In this study, lipidomics and proteomics characterization showed the presence of components with potential adjuvant effect in the bacterial membrane, enhancing the immunogenicity of the SUB-MSP1a antigen in mouse and pig vaccination models. In addition, this system provides a simple and cost-effective approach, to produce tick protective chimera antigens on the E. coli membrane, with endotoxin levels within the limits acceptable for recombinant vaccine formulations.
Chapter IV: General discussion
Chapter IV is presented as a general discussion with an overview of the challenges to the control of tick-borne diseases through vaccine intervention, and also the current status of vaccines for the control of tick-borne diseases [de la Fuente, J., Contreras, M., Estrada-Peña, A., Cabezas-Cruz, A. (2017). Targeting a global health problem: Vaccine design and challenges for the control of tick-borne diseases. Vaccine, (35) 5089–5094]. These vaccines could constitute the safest and most effective intervention for the control of tick-borne diseases, inducing a long-lasting immunity, and reducing or even preventing tick infestation, pathogen infection and transmission in humans, domestic and wild animals
Ludwig RENN, La guerra civil española. Crónica de un escritor en las Brigadas Internacionales, Madrid, Fórcola Ediciones, 2016. 721 pp. ISBN: 978-84-16247-58-5
Get PDFRadiofrequency electromagnetic fields and some cancers of unknown etiology: An ecological study
No full textSimultaneously with the increase of Radiofrequency Electromagnetic Fields (RF-EMF) in recent decades, there has been increasing concern about their potential relation with the etiology of several tumors. At this time, the techniques of spatial data analysis jointly with the study of the personal exposure to these fields offer a new approach to the problem.
This paper presents the results of a preliminary epidemiological study, combining Epidemiology, Statistics and Geographical Information Systems (GIS), in which we analyzed the correlation between exposure to RF-EMF in the city of Albacete (166,000 inhabitants, southeast Spain) and the incidence of several cancers with unspecific causes (lymphomas, and brain tumors).
We used statistical tools to analyze the spatial point patterns and aggregate data with the aim to study the spatial randomness and to determine the zones with the highest incidence from 95 tumors studied (65 lymphomas, 12 gliomas and 18 meningiomas). We also perform a correlation (Spearman) study between the personal exposure to RF-EMF in 14 frequency bands, recorded by an EME Spy 140 (Satimo) exposimeter in the city's administrative regions, and the incidence of the tumors registered from January 2012 to May 2015.
The studied cancer cases have a random spatial distribution inside the city. On the other hand, and by means of an ecological study, we verified that the exposure to RF-EMF registered in the city of Albacete shows little correlation with the incidence of the studied tumors (gliomas (? = 0.15), meningiomas (? = 0.19) and lymphomas (? = - 0.03)). The proposed methodology inaugurates an unexplored analysis path in this field
Cuestión prejudicial planteada mediante AJPI nº 2 de Santander de 10 de septiembre de 2014 (JUR 2015 1360169)
Get PDFEn esta nota se comenta la reciente STJUE de 26 de enero de 2017 en la que el TJUE resuelve una cuestión prejudicial de un Juzgado español que pregunta sobre la concordancia de determinadas cuestiones relativas al procedimiento de ejecución hipotecaria con la Directiva de cláusulas abusivas
La Junta del "Nuevo Código de Indias" (1776-1820): Observaciones y precisiones de revisión para una renovada interpretación
Get PDFThe study of the formation and the analysis of the content of as well known as New Code of Laws of the Indies, approved, sanctioned and promulgated by Charles IV of Spain, in 1792, but not published in general and jointly, it can reach new conclusions and a renewed historiographical interpretation of its origin, development and the causes of their secret force, withthe exception of twelve of its laws, mandated to apply by the American (Indian) governmental and judicial authorities, between 1789 and 1804. Years earlier, in 1776, Charles III, King of Spain, had ordered that the century-old Indian Compilation (1680), was to be added and illustrated with the provisions enacted later. But of the nine books compiled by order of Charles II, inthe late Seventeenth Century, the only one that ended up being proposed by the Board of the New Code (Junta del Nuevo Código), created for it in the late Eighteenth Century, and finally accepted, it was the Book I, which it was a compilation of the Bourbon legislation of ecclesiastical government of America, which revolved around its key institution, the Royal Patronage (Patronato Real). The consultation of the preserved acts of such Junta (1776-1785), together with the Book I project, proposed by Juan Crisóstomo de Ansotegui (1780), among other documentary sources, it is enable to clear many questions that hover over this legislative work: why it is an anachronistic and partial official Compilation and not a complete Code of Illustration; what the slowness of the Junta in its disputes with the Royal Council of the Indies was due; or what were, finally, the real causes, beyond traditional regalism, by the New Code not end up being published, when the French Revolution of 1789had kindled throughout Europe and threatened the stability of its ancient Monarchies absolutists.El estudio del proceso de formación y el análisis del contenido del conocido como Nuevo Código de Leyes de las Indias, aprobado, sancionado y promulgado por Carlos IV, en 1792, pero no publicado de forma general y conjunta, permite llegar a nuevas conclusiones y a una renovada interpretación historiográfica sobre su origen, su elaboración y las causas de su secretavigencia, excepción hecha de doce de sus leyes, mandadas aplicar, por parte de las autoridades gubernativas y judiciales indianas, entre 1789 y 1804. Años antes, en 1776, Carlos III había ordenado que la ya centenaria Recopilación de Indias, de 1680, fuese adicionada e ilustrada con las disposiciones normativas promulgadas con posterioridad. Pero, de los nueve libros recopilados por mandato de Carlos II, a finales del siglo xvii, el único que terminaría siendo propuesto por la Junta del Nuevo Código, para ello creada, en las postrimerías del xviii, y finalmente aceptado, fue el Libro I, que era una compilación de la legislación borbónica sobre el gobierno eclesiástico de América, que giraba alrededor de su institución clave, el Patronato Real. El manejo de las actas de dicha Junta que se conservan (1776-1785), junto con el proyecto de Libro I, propuesto por Juan Crisóstomo de Ansotegui (1780), entre otras fuentes documentales, posibilita despejar los múltiples interrogantes que se ciernen sobre dicha obra legislativa, cuales los de por qué se trata de una anacrónica y parcial Recopilación oficial y no de un completo Código ilustrado; a qué se debió la lentitud recopiladora de la Junta en sus disputas con el Consejo Real de las Indias; o cuáles fueron, finalmente, las verdaderas causas, más allá de las tradicionales regalistas, por las que no terminó siendopublicado, cuando ya la Revolución Francesa, de 1789, había prendido por toda Europa y amenazaba la estabilidad de sus vetustas Monarquías absolutista
El compositor castellano-manchego Tomás Barrera Saavedra
No full textLa presente tesis doctoral presenta una investigación sobre la vida y obra del compositor castellano-manchego Tomás Barrera Saavedra, una de las figuras más relevantes de la zarzuela, el género chico y la opereta de las primeras décadas del siglo XX. Además, su nombre es un aporte fundamental en la transformación de los problemas editoriales del teatro musical del momento y, sobre todo, pieza clave en el intrincado asunto de la fundación de la Sociedad de Autores Españoles.
Esta investigación construye, a modo de introducción, un marco contextual (Historia, Música y Literatura) sobre el que encuadrar la figura del maestro de La Solana para, de este modo, mostrar los resultados en relación a su perfil biográfico, su obra musical y su estilo; asimismo, se aporta el análisis de tres de las obras mejor tratadas por la crítica de la época: Emigrantes (1905), El celoso extremeño (1908) y Sueño de Pierrot (1914), con el que se contribuye a un examen pormenorizado de esas partituras para adentrarnos en su técnica creativa, y un estudio filológico de los textos dramáticos que sustentan su música para conocer a fondo la manera en la que el compositor penetra con su arte en el universo ficcional del teatro