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Detecting Protein-Ligand Interactions with Nitroxide Based Paramagnetic Cosolutes
No full textNMR spectroscopy techniques can provide important information about protein-ligand interactions. Here we tested an NMR approach which relies on the measurement of paramagnetic relaxation enhancements (PREs) arising from analogous cationic, anionic or neutral soluble nitroxide molecules, which distribute around the protein-ligand complex depending on near-surface electrostatic potentials. We applied this approach to two protein-ligand systems, interleukin-8 interacting with highly charged glycosaminoglycans and the SH2 domain of Grb2 interacting with less charged phospho-tyrosine tripeptides. The electrostatic potential around interleukin-8 and its changes upon binding of glycosaminoglycans could be derived from the PRE data and confirmed by theoretical predictions from Poisson-Boltzmann calculations. The ligand influence on the PREs and NMR-derived electrostatic potentials of Grb2 SH2 was localized to a narrow protein region which allowed the localization of the peptide binding pocket. Our analysis suggests that experiments with nitroxide cosolutes can be useful for investigating protein-ligand electrostatic interactions and mapping ligand binding sites
Ground- and ship-based microwave radiometer measurements during EUREC4A
No full textDuring the EUREC4A field study, microwave radiometric measurements were performed at Barbados Cloud Observatory (BCO) and aboard RV Meteor and RV Maria S Merian in the downstream winter trades of the North Atlantic. We present retrieved integrated water vapor (IWV), liquid water path (LWP), and temperature and humidity profiles as a unified, quality-controlled, multi-site data set on a 3 s temporal resolution for a core period between 19 January and 14 February 2020 in which all instruments were operational. Multi-channel radiometric measurements were performed at BCO and aboard RV Meteor between 22 and 31 GHz (K-band) and from 51 to 58 GHz (V-band). Combined radar–radiometer measurements of a W-band Doppler radar with a single-channel radiometer instrument were conducted at 89 GHz aboard RV Meteor and RV Maria S Merian. We present a novel retrieval method to retrieve LWP from single-channel 89 GHz measurements, evaluate retrieved quantities with independent measurements, and analyze retrieval uncertainties by site and instrument intercomparison. Mean IWV conditions of 31.8 kg m−2 match independent radiosoundings at BCO with a root-mean-square difference of 1.1 kg m−2. Mean LWP conditions in confidently liquid cloudy, non-precipitating conditions ranged between 63.1 g m−2 at BCO and 46.8 g m−2 aboard RV Maria S Merian. Aboard the ships, 90 % of LWP was below 120 g m−2 with a 30 % uncertainty for LWP of 50 g m−2. Up to 20 % of confidently liquid cloudy profiles ranged below the LWP detection limit due to optically thin clouds
Hill–Chao numbers allow decomposing gamma multifunctionality into alpha and beta components
No full textBiodiversity–ecosystem functioning (BEF) research has provided strong evidence and mechanistic underpinnings to support positive effects of biodiversity on ecosystem functioning, from single to multiple functions. This research has provided knowledge gained mainly at the local alpha scale (i.e. within ecosystems), but the increasing homogenization of landscapes in the Anthropocene has raised the potential that declining biodiversity at the beta (across ecosystems) and gamma scales is likely to also impact ecosystem functioning. Drawing on biodiversity theory, we propose a new statistical framework based on Hill–Chao numbers. The framework allows decomposition of multifunctionality at gamma scales into alpha and beta components, a critical but hitherto missing tool in BEF research; it also allows weighting of individual ecosystem functions. Through the proposed decomposition, new BEF results for beta and gamma scales are discovered. Our novel approach is applicable across ecosystems and connects local- and landscape-scale BEF assessments from experiments to natural settings
Hotspots of Floating Plastic Particles across the North Pacific Ocean
No full textThe pollution of the marine environment with plastic debris is expected to increase, where ocean currents and winds cause their accumulation in convergence zones like the North Pacific Subtropical Gyre (NPSG). Surface-floating plastic (>330 μm) was collected in the North Pacific Ocean between Vancouver (Canada) and Singapore using a neuston catamaran and identified by Fourier-transform infrared spectroscopy (FT-IR). Baseline concentrations of 41,600–102,700 items km–2 were found, dominated by polyethylene and polypropylene. Higher concentrations (factors 4–10) of plastic items occurred not only in the NPSG (452,800 items km–2) but also in a second area, the Papaha̅naumokua̅kea Marine National Monument (PMNM, 285,200 items km–2). This second maximum was neither reported previously nor predicted by the applied ocean current model. Visual observations of floating debris (>5 cm; 8–2565 items km–2 and 34–4941 items km–2 including smaller “white bits”) yielded similar patterns of baseline pollution (34–3265 items km–2) and elevated concentrations of plastic debris in the NPSG (67–4941 items km–2) and the PMNM (295–3748 items km–2). These findings suggest that ocean currents are not the only factor provoking plastic debris accumulation in the ocean. Visual observations may be useful to increase our knowledge of large-scale (micro)plastic pollution in the global oceans
Introducing the Video In Situ Snowfall Sensor (VISSS)
No full textThe open-source Video In Situ Snowfall Sensor (VISSS) is introduced as a novel instrument for the characterization of particle shape and size in snowfall. The VISSS consists of two cameras with LED backlights and telecentric lenses that allow accurate sizing and combine a large observation volume with relatively high pixel resolution and a design that limits wind disturbance. VISSS data products include various particle properties such as maximum extent, cross-sectional area, perimeter, complexity, and sedimentation velocity. Initial analysis shows that the VISSS provides robust statistics based on up to 10 000 unique particle observations per minute. Comparison of the VISSS with the collocated PIP (Precipitation Imaging Package) and Parsivel instruments at Hyytiälä, Finland, shows excellent agreement with the Parsivel but reveals some differences for the PIP that are likely related to PIP data processing and limitations of the PIP with respect to observing smaller particles. The open-source nature of the VISSS hardware plans, data acquisition software, and data processing libraries invites the community to contribute to the development of the instrument, which has many potential applications in atmospheric science and beyond
Somatosensory omissions reveal action-related predictive processing
No full textThe intricate relation between action and somatosensory perception has been studied extensively in the past decades. Generally, a forward model is thought to predict the somatosensory consequences of an action. These models propose that when an action is reliably coupled to a tactile stimulus, unexpected absence of the stimulus should elicit prediction error. Although such omission responses have been demonstrated in the auditory modality, it remains unknown whether this mechanism generalizes across modalities. This study therefore aimed to record action-induced somatosensory omission responses using EEG in humans. Self-paced button presses were coupled to somatosensory stimuli in 88% of trials, allowing a prediction, or in 50% of trials, not allowing a prediction. In the 88% condition, stimulus omission resulted in a neural response consisting of multiple components, as revealed by temporal principal component analysis. The oN1 response suggests similar sensory sources as stimulus-evoked activity, but an origin outside primary cortex. Subsequent oN2 and oP3 responses, as previously observed in the auditory domain, likely reflect modality-unspecific higher order processes. Together, findings straightforwardly demonstrate somatosensory predictions during action and provide evidence for a partially amodal mechanism of prediction error generation
Standardization of a CT Protocol for Imaging Patients with Suspected COVID-19: A RACOON Project
No full textCT protocols that diagnose COVID-19 vary in regard to the associated radiation exposure and the desired image quality (IQ). This study aims to evaluate CT protocols of hospitals participating in the RACOON (Radiological Cooperative Network) project, consolidating CT protocols to provide recommendations and strategies for future pandemics. In this retrospective study, CT acquisitions of COVID-19 patients scanned between March 2020 and October 2020 (RACOON phase 1) were included, and all non-contrast protocols were evaluated. For this purpose, CT protocol parameters, IQ ratings, radiation exposure (CTDIvol), and central patient diameters were sampled. Eventually, the data from 14 sites and 534 CT acquisitions were analyzed. IQ was rated good for 81% of the evaluated examinations. Motion, beam-hardening artefacts, or image noise were reasons for a suboptimal IQ. The tube potential ranged between 80 and 140 kVp, with the majority between 100 and 120 kVp. CTDIvol was 3.7 ± 3.4 mGy. Most healthcare facilities included did not have a specific non-contrast CT protocol. Furthermore, CT protocols for chest imaging varied in their settings and radiation exposure. In future, it will be necessary to make recommendations regarding the required IQ and protocol parameters for the majority of CT scanners to enable comparable IQ as well as radiation exposure for different sites but identical diagnostic questions
The effects of feeding sodium chloride pellets on the gastric mucosa, acid-base, and mineral status in exercising horses
No full textElectrolyte supplementation may be a risk factor for gastric mucosal lesions, but relevant evidence is limited in horses
The Good Disobedient Citizen? Students’ Perspectives on Good Citizenship in the International Civic and Citizenship Education Study 2016
No full textScholars of democratic citizenship and citizenship education have long discussed whether willingness to commit civil disobedience (CD) is an important attribute of citizens to defend and enforce democratic and human rights. In light of the youth protests of recent years, this article examines whether 14-year-old students consider CD important for being a good citizen. Using representative Danish, German, Dutch and Norwegian samples from the International Civic and Citizenship Education Study 2016, this study investigates dimensions of students’ concepts of citizenship and their relatedness to civic knowledge and support for equal rights. Confirmatory factor analysis established CD as a distinct dimension of good citizenship whose scale is metrically invariant across countries. However, linear regressions showed a negative impact of civic knowledge and support for equal gender rights on CD attitudes, raising the question if the empirical pattern of students supporting CD fits with theoretical assumptions of critical or transformative citizenship education