Brage Nord Open Research Archive
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Re-evaluation of Methionine and Isoleucine Requirements in Juvenile Hybrid Grouper Fed Protein-Bound and Crystalline Amino Acids Diets
Full text linkThis study determined the methionine (Met) and isoleucine (Ile) requirements of juvenile hybrid grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂ ) using graded protein-bound amino acid (PBAA) diets. Two feeding trials were conducted, each including a crystalline amino acid (CAA) diet at the same dose as the corresponding PBAA diet for direct comparison. Seven PBAA-Met (8.2–17.1 g/kg) and six PBAA-Ile (15.3–20.7 g/kg) diets were tested, together with two CAA-based diets (Met-14.9 g/kg; Ile-20.0 g/kg). A total of 266 fish (initial weight: 12.0 ± 0.1 g/fish) were fed to apparent satiation for seven (Met trial) or eight (Ile trial) weeks. The optimal Met and Ile requirements for PBAA-fed fish were estimated at 11.26 g/kg and 19.87 g/kg, respectively. Fish fed CAAMet showed lower protein efficiency ratio (PER) and protein productive value (PPV), but higher hepatic glutamate-pyruvic transaminase (GPT) and glutamic-oxaloacetic transaminase (GOT) activity than those fed PBAAMet. Conversely, fish fed CAA-Ile showed significantly lower weight gain (WG) (p < 0.001) and significantly higher ammonia nitrogen excretion (p<0.05), along with a decreasing trend in PER, PPV, and a higher feed conversion ratio (FCR), compared to fish fed PBAAD-Ile. The postprandial essential amino acid (EAA) profile revealed rapid spikes and declines in serum AA levels within 6 h in fish fed CAA diets, indicating rapid, yet transient absorption and increased catabolism, corroborated by elevated hepatic GPT and GOT activities. In contrast, fish fed PBAA diets exhibited increased hepatic expression of mtor and igf1, suggesting enhanced protein synthesis and nutrient utilization efficiency. Additionally, the expression of intestinal pept1 and slc1a1 was significantly upregulated in fish fed 10.4 g/kg PBAAD-Met, but not in those fed CAA-Met 14.9, correlating with improved amino acid absorption and feed efficiency. These findings demonstrate that CAA-based formulations overestimate Met requirements due to transient metabolic imbalances and increased oxidation, whereas Ile metabolism appears less affected by the AA form. The study also highlights that serum-free AA dynamics may serve as potential biomarkers for AA bioavailability, providing a framework for optimizing precision nutrition strategies and improving nitrogen utilization efficiency in aquaculture feed formulation.publishedVersio
Navigating the ethical “space in-between” nurses’ lived experiences in forensic inpatient care interpreted through Løgstrup’s ethical philosophy
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Defining jurisdictional boundaries in thirteenth-century Danish and Norwegian town law
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The symphony of functionalities: Dismantling engagements with environment and strategizing in small and medium-sized accounting practices
Full text linkDoctoral thesis (PhD) - Nord University, 2025publishedVersio
The role of SAI in the context of war: evidence from Ukraine
Full text linkAuthor’s accepted manuscript (postprint).This is an Accepted Manuscript of an article published by Emerald Publishing Limited in Journal of Public Budgeting, Accounting and Financial Managementon 21/1/2025.Available online: https://doi.org/10.1108/JPBAFM-12-2024-0246.acceptedVersio
Microbial biomass carbon distribution under agroforestry practices and soil depth variations in Southern Ethiopia
Full text linkAuthor’s accepted manuscript (postprint).This is an Accepted Manuscript of an article published by Springer Nature in Agroforestry Systems on 27/2/2025.Available online: https://doi.org/10.1007/s10457-025-01133-8.acceptedVersio
Critical gaps in the global fight against locust outbreaks and addressing emerging challenges
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The integration experiences of older Finnish re-migrants: ‘Embraced by the Swedes … but Finland is my home country’
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