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結合生理回饋之智慧穿戴裝置應用於全膝關節置換術後病患復健運動之成效
No full text[[abstract]]探討結合生理回饋之智慧穿戴裝置，應用於全膝關節置換術後病患復 健運動，改善膝關節功能之成效
如何找到最適合的防噪音耳塞
No full text[[abstract]]在工商業社會下人們常因各種原因，使自己處於長期噪音環境中，而不同的
噪音環境有各種噪音聲學特性及頻率，容易造成聽力損傷與後續的問題。為了減
緩噪音環境對聽力的損傷，因此，我們想尋求以最簡易的購得來源、戴起來最舒
適與最低的花費的耳塞，幫助人們能以最簡單且經濟實惠的方式來保護耳朵。本
研究以主觀與客觀兩方面來測試 30 位年紀為 19-20 歲的受試者的市售耳塞使用
效果，主觀測試為測試耳塞配戴前後的聽力閾值之相差值與問卷調查，客觀測試
包括耳道容積測試與耳塞配戴前後在真耳中的相差值，最後使用 two-way
ANOVA 分配進行統計分析，來探討(1)耳道容積與真耳測試相差值之相關性，(2)
耳道容積與聲場測試相差值的相關性。由本研究結果可發現，耳塞對於遮蔽高頻
聲音的效益普遍較佳，只有部分耳塞對於遮蔽中、低頻噪音較有效用。耳塞的遮
蔽效益與使用者配戴耳塞的位置、形狀以及材料使用的不同有顯著相關性，因此
使用者需配戴符合自己耳道容積大小、配戴習慣的耳塞，以減少因使用者配戴因
素影響遮蔽效果
醫學暨健康學院物理治療學系
No full text[[abstract]]本研究旨在探討台灣和美國學齡前兒童，由不同文化做變項，探究其負向情緒能力、情
緒調節之理解及氣質的相關及異同。[[note]]高
Half a century of Sri Lanka research: Subjects, researchers, institutions, journals and impact (1973-2019)
No full text[[abstract]]Introduction: Bibliometric analyses of research in Sri Lanka, a lower-middle income island nation in South Asia, has focused mainly on medical research, concluding that there is a need for increased research productivity and impact, and for local solutions to health concerns. There has been no general bibliometric analysis across scientific disciplines in the nation, or any study that covers a long period of time to identify general time trends. Objective: To measure and analyse Sri Lanka research by focusing on subjects, authors, institutions, journals and citation for half a century. Methods: We used an advanced search method to extract publications with the word “Sri Lanka” in the SCI-EXPANDED, and calculated indicators such as total citations from Web of Science Core Collection since publication year to the end of 2019, citations in 2019, and mean citations per publication. Journal data were taken from 2019 Journal Citation Report. Affiliation re-classification was done to ensure consistency regarding the origin of all publications. Publications were further analysed based on collaboration, and first and corresponding authorship. Results: We retrieved 16 069 publications in 19 document types (77 % articles). Corrections had the highest number of authors per publication (616) followed by articles (116). Four articles had more than 5 000 authors and 593 articles had more than 1 000 authors. The highest citations in this database were for international megaprojects where Sri Lanka authors played minor roles. The UK had the most collaborative articles with Sri Lanka (19 %). The articles were published in 3 051 journals across 177 Web of Science categories. The category of Public, environmental and occupational health, with 193 journals, had 6.7 % of all articles, followed by environmental sciences (6.6 %). Conclusion: Sri Lanka has an unusually strong pattern of participating as small role players in international megaprojects about health and physics. Sri Lanka authors should be encouraged to expand their horizons by researching non-applied fields that are the basis of all innovation; to strengthen their own journals so that they have better visibility and impact, and to improve their positions in international projects that are published in larger journals
Novel anti-aging herbal formulation Jing Si displays pleiotropic effects against aging associated disorders
No full text[[abstract]]Common characteristics of aging include reduced somatic stem cell number, susceptibility to cardiac injuries, metabolic imbalances and increased risk for oncogenesis. In this study, Pleiotropic anti-aging effects of a decoction Jing Si herbal drink (JS) containing eight Traditional Chinese Medicine based herbs, with known effects against aging related disorders was evaluated. Adipose derived mesenchymal stem cells (ADMSCs) from 16 week old adult and 24 month old aging WKY rats were evaluated for the age-related changes in stem cell homeostasis. Effects of JS on self-renewal, klotho and Telomerase Reverse Transcriptase expression DNA damage response were determined by immunofluorescence staining. The effects were confirmed in senescence induced human ADMSCs and in addition, the potential of JS to maintain telomere length was evaluated by qPCR analysis in ADMSCs challenged for long term with doxorubicin. Further, the effects of JS on doxorubicin-induced hypertrophic effect and DNA damage in H9c2 cardiac cells; MPP+-induced damages in SH-SY5Y neuron cells were investigated. In addition, effects of JS in maintaining metabolic regulation, in terms of blood glucose regulation in type-II diabetes mice model, and their potential to suppress malignancy in different cancer cells were ascertained. The results show that JS maintains stem cell homeostasis and provides cytoprotection. In addition JS regulates blood glucose metabolism, enhances autophagic clearances in neurons and suppresses cancer growth and migration. The results show that JS acts on multiple targets and provides a cumulative protective effect against various age-associated disorders and therefore it is a candidate pleiotropic agent for healthy aging
Targeting glycolysis in Th2 cells by pterostilbene attenuates clinical severities in an asthmatic mouse model and IL-4 production in peripheral blood from asthmatic patients
No full text[[abstract]]Asthma, a major non-communicable disease, affects both adults and children and is associated with high morbidity compared with other chronic diseases. The glycolysis-associated activation of type 2 helper T (Th2) cells is the critical immunopathological mechanism involved in asthma deterioration. Long-term use of steroids as a medical treatment for asthma induces side effects and resistance. Pterostilbene (PS), a stilbenoid compound found in blueberry and vines, exhibits antihyperglycemic and anti-inflammatory properties. Thus, we hypothesized that the modulation of T cell immunity by PS may be an applicable intervention to treat asthma. Airway hyperresponsiveness, interleukin (IL)-4 and IL-13 levels, IgE, IgG, pulmonary infiltrated monocytes and eosinophils, and mucosubstances were measured in house dust mite (HDM)-induced asthmatic mice under PS treatment. Bioenergetic metabolism, PI3K-mTOR signalling, GATA3 expression and histone acetylation in PS-treated Th2 cells were investigated. PS improved HDM-induced pulmonary allergic airway inflammation by inhibiting Th2 cell and eosinophil accumulation in HDM asthmatic mice both in the preventive and therapeutic models. Targeting glycolysis resulted in IL-4 inhibition via the downregulation of mTOR, GATA3 and histone acetylation in PS-treated Th2 cells. Glucose supplementation reversed the inhibitory effect of PS on Th2 cells in vitro. Adoptive transfer with glucose-treated Th2 cells enhanced Th2 activation and eosinophilic accumulation in PS-treated asthmatic mice. Furthermore, PS significantly inhibited IL-4 production of CD4+ T cells from the peripheral blood mononuclear cells of patients with asthma. PS attenuates HDM-induced asthma via the inhibition of the Glut1/mTOR/GATA3 axis in Th2 cells, which supports the potential pharmaceutical application of PS treatment for asthma
The fciTABC and feoABI systems contribute to ferric citrate acquisition in Stenotrophomonas maltophilia
No full text[[abstract]]Stenotrophomonas maltophilia, a member of γ-proteobacteria, is a ubiquitous environmental bacterium that is recognized as an opportunistic nosocomial pathogen. FecABCD system contributes to ferric citrate acquisition in Escherichia coli. FeoABC system, consisting of an inner membrane transporter (FeoB) and two cytoplasmic proteins (FeoA and FeoC), is a well-known ferrous iron transporter system in γ-proteobacteria. As revealed by the sequenced genome, S. maltophilia appears to be equipped with several iron acquisition systems; however, the understanding of these systems is limited. In this study, we aimed to elucidate the ferric citrate acquisition system of S. maltophilia.
Methods
Candidate genes searching and function validation are the strategy for elucidating the genes involved in ferric citrate acquisition. The candidate genes responsible for ferric citrate acquisition were firstly selected using FecABCD of E. coli as a reference, and then revealed by transcriptome analysis of S. maltophilia KJ with and without 2,2′-dipyridyl (DIP) treatment. Function validation was carried out by deletion mutant construction and ferric citrate utilization assay. The bacterial adenylate cyclase two-hybrid system was used to verify intra-membrane protein–protein interaction.
Results
Smlt2858 and Smlt2356, the homologues of FecA and FecC/D of E. coli, were first considered; however, deletion mutant construction and functional validation ruled out their involvement in ferric citrate acquisition. FciA (Smlt1148), revealed by its upregulation in DIP-treated KJ cells, was the outer membrane receptor for ferric citrate uptake. The fciA gene is a member of the fciTABC operon, in which fciT, fciA, and fciC participated in ferric citrate acquisition. Uniquely, the Feo system of S. maltophilia is composed of a cytoplasmic protein FeoA, an inner membrane transporter FeoB, and a predicted inner membrane protein FeoI. The intra-membrane protein–protein interaction between FeoB and FeoI may extend the substrate profile of FeoB to ferric citrate. FeoABI system functioned as an inner membrane transporter of ferric citrate.
Conclusions
The FciTABC and FeoABI systems contribute to ferric citrate acquisition in S. maltop
探究不同的教學語言及訓練教學方案對外籍照顧服務員口腔照 護學習成效之影響
No full text[[abstract]]臺灣高齡者的長期照護工作多仰賴外籍照顧服務員，然因其工作繁重，受限於時間及母語講師人力的缺乏，職前訓練與在職訓練均有所不足。本研究目的即在設計出一套適合外籍照顧服務員的在職訓練方案，解決資源限制的問題，且能因應其語言不熟悉的障礙提升教學成效。本研究採類實驗設計，以臺北市、新北市及基隆市住宿式長期照顧機構325位外籍照顧服務員為研究對象，參與者被分為四組，接受不同的在職訓練方案，並於在職訓練前、後進行口腔照護能力相關認知、情意及技能的評估。四種不同的在職訓練方案由教學語言（母語／華語）與訓練教學方案（互動式多媒體教學法／傳統式課堂教學法）兩個變項所構成，分別為：母語互動式多媒體教學組、華語互動式多媒體教學組、母語傳統式課堂教學組、華語傳統式課堂教學組。結果發現使用外籍照顧服務員的母語教學有相對的教學成效優勢，其全面性的學習成效較佳。其次，不同教學方式在認知、情意及技能向度上會展現不同的優勢：互動式多媒體教學法在認知及技能向度上較傳統式課堂教學法佳；若欲使用華語教學提升情意向度的學習成效，則傳統式課堂教學法較互動式多媒體教學法具有優勢。根據本研究的發現，建議實務上應隨著在職訓練的目標彈性地調整教學法，選用合適的教學法能使有限的資源於照護在職訓練工作上發揮最大的效用
Induction of Heme Oxygenase-1 by 15d-Prostaglandin J 2 Mediated via a ROS-Dependent Sp1 and AP-1 Cascade Suppresses Lipopolysaccharide-Triggered Interleukin-6 Expression in Mouse Brain Microvascular Endothelial Cells
No full text[[abstract]]Heme oxygenase-1 (HO-1) has been shown to exert antioxidant, anti-inflammatory, and anti-apoptotic effects in various types of cells. Therefore, the induction of HO-1 is an excellent rationale for the development of protective drugs. 15-Deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2) can modulate the expression of antioxidant defense proteins and be beneficial for neuroinflammation. Brain endothelial cells play an important role in the pathophysiology of brain disorders. Whether 15d-PGJ2 can induce HO-1 expression and protect against the inflammatory responses in mouse brain microvascular endothelial (bEnd.3) cells remains unclear. Here, we reveal that 15d-PGJ2 stimulated HO-1 protein and mRNA expression in a time- and concentration-dependent manner in bEnd.3 cells, which was attenuated by diphenyleneiodonium chloride (DPI) and MitoTempo. Thus, activation of NADPH oxidase (NOX)- and mitochondria-derived reactive oxygen species (ROS) mediated 15d-PGJ2-induced HO-1 expression. ROS generation could cause phosphorylation of protein kinase C (PKC)δ, leading to HO-1 expression, which was suppressed by Rottlerin (selective inhibitor PKCδ), DPI, and MitoTempo. We further demonstrated that phosphorylation of c-Jun N-terminal kinase (JNK)1/2 participated in 15d-PGJ2-upregulated HO-1 expression, which was blocked by SP600125 or Rottlerin. Moreover, 15d-PGJ2-induced HO-1 expression was mediated through the activation of c-Jun (a subunit of activator protein 1 (AP-1)) and specificity protein 1 (Sp1), leading to their interaction with the HO-1 promoter, revealed by chromatin immunoprecipitation assay, which was attenuated by SP600125, Mithramycin A, or Tanshinone II A. We further verified the anti-inflammatory effect of HO-1 expression. Our results showed that 15d-PGJ2-induced HO-1 could mitigate the lipopolysaccharide-triggered interleukin-6 expression and secretion, as measured by an ELISA assay kit. These results suggest that 15d-PGJ2-induced HO-1 expression is mediated through the activation of NOX- and mitochondria-derived ROS-dependent PKCδ/JNK1/2/Sp1 and the AP-1 signaling pathway and protects against inflammatory responses in bEnd.3 cells