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ループスアンチコアグラント検査におけるクロスミキシング試験の標準化に関する研究
Get PDFクロスミキシング試験(CMT)における種々の活性化部分トロンボプラスチン時間(APTT)試薬とさまざまな凍結乾燥市販正常血漿の有用性および判定方法について検討した。APTT-LA測定に有用なAPTT試薬の検討では、ループスアンチコアグラント(LA)検査が陽性の患者21例の血漿を用い、健常人50名を対照群とした。測定値の比較ではPTT-LAと最も近似した測定値を示したのはAPTT-3であり、最も乖離したのはAPTT-5であった。日本血栓止血学会標準化委員会(JSTH-SSC)がLA試薬として推奨するPTT-LAとの相関性はAPTT-4が相関係数r=0.883と最も高く、次いでAPTT-3が良好であった。また、LA検査としてCMTに用いるコントロール血漿の検討では、凝固スクリーニング検査の結果はいずれのコントロール血漿も正常域であったが、APTT値においてGeorge King NORMAL PLASMAとPOOL NORM(RD)は他の正常血漿に比較してやや延長していた。内因系凝固因子活性はRDが他のコントロール血漿と比較して第VIII因子および第IX因子、第XII因子活性が低下していた。特に、第IX因子および第XII因子活性値はそれぞれ基準値以下であった。博士（医学）旭川医科大
EphA7+perivascular cells as myogenic and angiogenic precursors improving skeletal muscle regeneration in a muscular dystrophic mouse model. （EphA7陽性周細胞は、筋ジストロフィーマウスモデルにおいて、骨格筋再生能を改善する）
Get PDFSkeletal muscle has a capacity for muscular regeneration mediated by satellite cells (SCs) and non-SCs. Although it is proposed that non-SCs are attractive therapeutic targets for dystrophies, the biological properties of these cells remain unclear. We have recently identified novel multipotent pericytes (PCs), capillary stem cells (CapSCs) derived from the microvasculature. In the present study, we determined if CapSCs contributed to myogenic regeneration using muscular dystrophy mouse model. CapSCs were isolated as EphA7+NG2+PCs from the subcutaneous adipose tissues of GFP-transgenic mice. Co-culture with C2C12 myoblast cells showed that CapSCs effectively enhanced myogenesis as compared to controls including EphA7- PCs and adipose stromal cells (ASCs). CapSCs transplanted in cardiotoxin-injured gastrocnemius muscles were well differentiated into both muscle fibers and microvessels, as compared to controls. At three weeks after cell-transplantation into the limbs of the mdx/utrn-/-mouse, CapSCs increased the number of GFP+myofibers along with dystrophin expression and the area size of myofibers, and also enhanced the muscular mass and its performance, assessed by treadmill test as compared to controls. In conclusion, CapSCs have potent myogenic regeneration capacity and improved the pathological condition in a muscular dystrophy mouse. Thus, CapSCs are an attractive cellular source in regenerative therapy for muscular dystrophy.博士（医学）旭川医科大
Spasticity and Range of Motion Over Time in Stroke Patients who Received MultiI542:I548ple Dose Botulinum Toxin Therapy （複数回ボツリヌス療法を実施した脳卒中患者における痙縮および関節可動域の経時的変化）
Get PDFObjective: This study examined how the effects of botulinum toxin therapy changed over time by sequential evaluation of clinical improvements in spasticity and contracture in 24 chronic-stage stroke patients on repeated botulinum toxin therapy who were receiving fewer rehabilitation interventions.
Methods: Botulinum toxin injection was administered into the spastic muscle of the paralyzed upper or lower limb 5 times with at least 3-month intervals. Modified Ashworth Scale and range of motion were measured before and 2 weeks after each dose in the extremities to compare the first measurement value with subsequent values. Each predose value was also compared with the first predose value.
Results: Compared with predose scores, Modified Ashworth Scale significantly improved in all flexors after 2 weeks from the first to fifth doses. Range of motion significantly improved in wrist dorsiflexion and ankle dorsiflexion. Comparison of values before each dose versus the first predose value showed significant improvement both in the Modified Ashworth Scale score of wrist flexors, finger flexors, and ankle planter flexors, and the range of motion of elbow extension, wrist dorsiflexion, and ankle dorsiflexion.
Conclusion: The comparison of predose values versus 2-week postdose values indicated that the effect of botulinum toxin formulation would not lessen after repeated injections with continuous improvements of Modified Ashworth Scale and range of motion. The comparison of predose values versus the first predose value also suggested that multiple injections of botulinum toxin formulation could be more effective in reducing spasticity and increasing the range of motion than a single injection.博士（医学）旭川医科大