33145 research outputs found
Sort by
Ruthenium(ii)-bisdemethoxycurcumin conjugate complexes as potent antitumour agents through simultaneous inhibition of 20S proteasome and HMG-CoA reductase
No full textThe synthesis of new conjugated bisdemethoxycurcumin ligands, each incorporating a distinct acyl substituent-cyclic, aliphatic, heteroaromatic, or branched-in place of the native hydroxyl groups, has been reported. These ligands were employed to prepare the corresponding Ru(ii)-p-cymene complexes, which were comprehensively characterized by FT-IR, NMR spectroscopy, elemental analysis, and ESI-MS. Single-crystal X-ray diffraction was used to elucidate the solid-state structures of two ligands and two metal complexes. Density Functional Theory (DFT) calculations provided further insights into the structural and electronic features of both the free ligands and their complexes. The anticancer potential of the Ru(ii)-cymene compounds was assessed in vitro against a panel of human cancer cell lines (HepG2, Caco-2, and MCF-7), as well as non-tumorigenic controls. The complexes exhibited selective, cell-type-specific cytotoxicity, primarily mediated through proteotoxic stress-evidenced by proteasome inhibition and p62 accumulation-and HMG-CoA reductase-dependent downregulation of PCNA expression
Nutritional characterization of a fermented beverage from donkey milk
No full textThe present work is part of the Tool4MEDLife project (PRIMA program), which aims to in-crease awareness about the Mediterranean diet thanks to the production of innovative foods and educational toolkits. Donkey milk, due to its potential hypoallergenic properties, has been sug-gested as a good alternative for feeding young children affected by cow milk protein allergy (CMPA) when it is not possible to breastfeed. Furthermore, thanks to the presence of nutraceu-tical molecules with antioxidant, anti inflammatory, antimicrobial, antiviral, and antitumor activities, donkey milk is interesting also for elderly people. Considering the high quality char-acteristics associated with fermented milk, the aim of this work was the production of ferment-ed donkey milk by selected Lactic Acid Bacteria (LAB) able to synthesize folates (vitamin B9). The final product was also fortified with vitamin D3 and calcium. The levels of folates of natu-ral origin represent an optimal alternative to synthetic folate-fortified products, which have limited bioavailability, and possibly lead to the accumulation of unmetabolized folic acid. Five fresh pasteurized donkey milk samples, added with vitamin D3, calcium carbonate, Streptococ-cus thermophilus, Lactobacillus delbrueckii ssp. bulgaricus and Lactobacillus plantarum IMC 509 (Synbiotec), were fermented at a constant temperature of 38-40°C for 5 hours. Folate con-tent on the final product was determined by RP HPLC (Agilent 1260 Infinity LC system), using a column C18 HiQSil HS, 5 μm, 4.6 mm i.d., 250 mm (Kya Tech Corporation, Tokyo, Japan).
The fermentation process led to the production of a fermented beverage with a final pH value of 4.4. The determination of folates by RP HPLC indicated mainly the presence of tetrahydro-folate (5.3 ± 0.081 M) which is a form of folate with high bioavailability. Other water soluble vitamins found in the final fermented donkey milk were vitamin C (110.16 ± 13.5 M), nicotin-ic acid, and nicotinamide (52.7 ± 0.05 M and 7.36 ± 0.15 M respectively), and also vitamin B12 (1.69 ± 0.022 M). Comparing the above results with the water soluble vitamin content of donkey milk present in the literature, it can be noticed that the vitamin content found in the fermented donkey milk beverage is considerably higher. These preliminary results indicated that the LABs used in these experiments are very promising to produce fermented donkey milk with enhanced nutraceutical properties.
Research supported by: PRIMA, Partnership for Research and Innovation in the Mediterranean Area supported by the European Union - Section 2, Tool4MEDLife Project - PRIMA22_00088 - CUP: J13C23000150002
Sex-related anti-nociceptive activity of a flavonoid-based formulated extract from citrus peels (Gold Lotion): new insights into a rat model
No full textInflammatory pain is a worldwide health problem, affecting primarily women. Nonetheless,
most research conducted in this field has involved male subjects, and only 10% of
the results have been obtained using females. Gold Lotion (GL) is a formulated product
made from the peels of six citrus fruits that have been proven, via experimental data, to
possess several biological properties, such as anti-inflammatory, anticancer, and antibacterial
activities. This research aims to investigate the response to GL administration in
two models of peripheral inflammation to assess potential sex differences. Carrageenan
was used to induce mechanical allodynia and maximal edema within hours, serving as
a model of acute inflammation, while Complete Freund’s Adjuvant (CFA) was used to
obtain similar results within days, resulting in a model of chronic inflammation. Acute
GL administration reduced mechanical allodynia in both models and both sexes, with no
anti-inflammatory effects observed. In conclusion, this study demonstrates the potential
of GL to alleviate inflammatory pain in both males and females, thereby broadening its
therapeutic applications
LEGGERE L’ARCHITETTURA, SCRIVERE LA STORIA. Intervista a GIORGIO CIUCCI
No full textProvare a restituire la biografia intellettuale di una personalità schiva come quella di Giorgio Ciucci significa restituire alcune pagine salienti della storia dell’architettura del secondo Novecento. La sua amicizia fin dagli studi universitari con Manfredo Tafuri a Roma e il gruppo Stass, quindi l’insegnamento all’Iuav di Venezia di Giuseppe Samonà e Carlo Aymonino, la collaborazione con la rivista “Oppositions” e il sodalizio con Peter Eisenman, gli studi su Le Corbusier, i CIAM, Giuseppe Terragni e infine il lavoro svolto a Roma Tre e all’Accademia di San Luca hanno reso Ciucci noto come storico autorevole per il suo cechoviano “distacco partecipato”. Difatti, come scrivono Mastrigli e Orazi, «Giorgio Ciucci è sempre riuscito a mantenere una certa distanza dalle cose, persino un vero e proprio distacco, che gli ha consentito tanto l’analisi lucida
che lo sguardo omnicomprensivo, tanto il giudizio sferzante quanto l’ironia. Così, anche il tono colloquiale di una conversazione, densa ma tutto sommato informale, lascia intravvedere i presupposti di una scrittura o anche di più: di uno stile»
Commento all'art. 175 "Programmazione, valutazione preliminare, controllo e monitoraggio" in materia di partenariato pubblico-privato e concessioni
No full textLa collocazione delle regole dedicate alle differenti fasi che riguardano un progetto di partenariato pubblico-privato (PPP) costituisce già una novità; con il Codice vigente, infatti, si è operata un’inversione sistematica rispetto al precedente impianto normativo: le disposizioni generali precedono la disciplina delle figure contrattuali tipiche di PPP (Cons. Stato, Relazione sullo schema definitivo del Codice, 2022, 203; sul rapporto fra le figure di PPP e, in particolare, sulle peculiarità di concessione e finanza di progetto cfr. G. Manfredi, Note su finanza di progetto, concessioni, partenariato, in Urb. App., 2024, p. 457; M. Ricchi, Struttura e semantica del PPP: le concessioni e la finanza di progetto nel nuovo Codice, il d.lgs. n. 36/2023, Ibid., p. 307). Si prevede una disciplina puntuale delle fasi di programmazione, valutazione preliminare, controllo e monitoraggio in caso di PPP, ed in questo senso si innova rispetto alla previgente legislazione, la quale si limitava a prevedere -nella norma dedicata alle procedure per la scelta dell’operatore economico cui affidare il contratto di PPP- la necessità di una istruttoria (“adeguata”) ai fini della scelta dell’operatore economico, ed una scarna regola sul controllo, di competenza dell’”amministrazione aggiudicatrice”, da esercitare sull’attività del concessionario, con l’obbligo per quest’ultimo di collaborare ai fini del “monitoraggio” (art. 181, d.lgs. 18 aprile 2016, n. 50). Nella logica della autonomia ed autosufficienza (“autoconclusività”) del Libro IV, invece, l’art. 175 dedica autonomo spazio alla programmazione (co. 1), alla valutazione preliminare (co. 2); alla fase consultiva (co. 3); alle fasi di controllo (co. 5 e 6) e di monitoraggio (co. 7), peraltro rimesse alla competenza di distinti soggetti (in tal modo distinguendo nettamente i due momenti). Tale innovativa regolamentazione risponde alla necessità di maggiore certezza giuridica e trasparenza nell’interesse dei soggetti coinvolti nel progetto di PPP, in coerenza con le indicazioni fornite dalla legge delega (art. 1, co. 2, l. 21 giugno 2022, n. 78). Il d.lgs. 31 dicembre 2024, n. 209 (di seguito, il “Correttivo”) è intervenuto sulla disposizione in commento, prevedendo significative modifiche rispetto alla fase consultiva (non solo modificando il co. 3, ma altresì sopprimendo il co. 4); nonché introducendo misure di semplificazione ed armonizzazione della normativa (nuovo co. 9-bis)
Riformismo. Note sul campo semantico di un termine
No full textInventario del Fondo
“Federazione Laburista, 1994 - 2000
Retrospective analysis of compliance with Reg. EC 1/2005: examination of Trade Control and Expert System for the transport of horses to and from Southern Italy
No full textThis study retrospectively analysed horse movements recorded in the European Trade Control and Expert System (TRACES) arriving in, departing from and crossing Apulia, during 2022–2023. Data from 290 TRACES were analysed to identify the most common inaccuracies and regulatory non-compliances, as well as the difference in journey conditions between horses that did and did not arrive at a slaughterhouse. The dataset included 3254 horses recorded in the 290 TRACES, of which 2946 were for slaughter. Descriptive statistics, chi-square, Kruskal-Wallis, or Mann-Whitney tests were performed. The most common routes for slaughter were from Poland and France to Apulia, while for other destinations were from Italy to Germany. Horses transported for slaughter were moved in significantly larger groups (mean 22 vs. 2 horses) and given less space per animal (1.57 vs. 3.25 m2) compared to other destinations (p < 0.001). Regardless of destinations and type of horses, namely registered and non-registered, the analysis revealed that journey durations, conditions, inaccuracies, and regulatory non-compliances were similar. Furthermore, 75 TRACES incorrectly classified horses as Registered when they were shipped for slaughter, which impedes verification of welfare standards. Many of the inaccuracies and non-compliances documented were associated with a specific transporter (p < 0.001). Our findings emphasise that TRACES needs improvements: many data fields were incorrectly completed, likely due to its complexity and vague policies. Shorter journeys and more space should be enforced to protect horse welfare during transport. Additionally, the TRACES’ portal for horses should ensure equal welfare standards for all transported horses, regardless of registration or destination
Professional Roles, Skills and Advanced Educational Programs of Breast Cancer Nurses: A Scoping Review
No full textAIM: To define the role, competencies and clinical focus of specialised breast cancer nurses and to explore post-basic academic training programmes available in Western countries. DESIGN: A scoping review was reported following the PRISMA-ScR framework and aligned with JBI methodology. METHODS: The research question was formulated using the Population, Concept and Context model. Searches were performed across four databases (Medline, Embase, Cochrane Library, CINAHL and Gray Literature) between July and September 2024. Article screening and data extraction were independently performed by two authors. Descriptive analysis was employed to interpret the data. RESULTS: From an initial 1499 records, 143 studies were selected, with 16 included in the final analysis. The studies, published between 2001 and 2024, evaluated the role of breast cancer nurses in oncology, focusing on their impact on patient care, health outcomes, quality of life, psychological support and breast cancer nurses-led interventions. Two studies assessed educational programmes, identifying a diverse range of postgraduate degrees and specialised training options tailored to meet the specific needs of this essential nursing role. CONCLUSIONS: Breast cancer nurses are crucial in delivering comprehensive care and improving QoL for breast cancer patients. They contribute to enhancing clinical outcomes through patient education and support. Ongoing professional development and expanded education are essential to meet evolving patient needs. IMPLICATIONS FOR THE PROFESSION: Breast cancer nurses play a pivotal role in improving patient outcomes by delivering education, psychological support and holistic care. To keep pace with the increasing complexity of breast cancer management, it is essential to prioritise expanded academic training and continuous professional development. These efforts will ensure BCNs are well prepared to address evolving patient needs and provide high-quality, comprehensive care. PATIENT OR PUBLIC CONTRIBUTION: No patient or public contribution
Giancarlo De Carlo. Il Campus universitario di Urbino
No full textNel 1951 Carlo Bo, rettore dell’Università di Urbino, invita per la prima volta Giancarlo De Carlo a Urbino per discutere di un “progetto” - che oggi possiamo definire “strategico”, per l’Ateneo e il territorio che l’ospita - dove i propri piani di ampliamento potessero annodarsi alla rigenerazione di una città fortemente legata alla presenza dell’università. Così l’anno successivo, nel ’52, Bo affida a De Carlo il primo incarico: il recupero dell’antica sede centrale dell’Università di Urbino, nel centro storico. Il progetto per Palazzo Bonaventura (1952-’60), già dimora dei Duchi di Montefeltro, rappresenta dunque l’inizio di un lungo rapporto che lega la vita e l’opera di De Carlo a Urbino, all’Ateneo e alla città
Giovanni Vaccarini. Palazzina adriatica
No full textUn incontro con Giovanni Vaccarini all’interno dell’edificio residenziale denominato ‘De Amicis 154’, appena dietro
il lungomare di Pescara, recentemente ultimato.
Vaccarini è uno dei post-giovani architetti più importanti della East Coast Adriatica. Con Anna Rita Emili lo abbiamo selezionato per il settimo numero di Enter-Vista in un momento significativo per lui, per le opportunità realizzative che sta avendo, per le pubblicazioni, sul suo lavoro, che stanno uscendo e i riconoscimenti ottenuti.
Contributi di Brunetto de Battè, Federico Bilò, Giulia Menzietti, Gianluigi Mondaini, Roberta Lucente