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Outcomes of RDAVR with coronary revascularization: 3-year results from the German INCA registry
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Climate and sustainable tourism in João Pessoa: a comparative study with Salvador and Rio de Janeiro, Brazil
No full textThis study aims to analyze how the climatic conditions in the city of João Pessoa, Brazil, influence sustainable tourism, with a specific focus on Climate–Tourism/Transfer–Information–Scheme (CTIS), Physiologically Equivalent Temperature (PET), and rainfall patterns. It also compares these aspects with those of Salvador and Rio de Janeiro to identify climatic patterns, local challenges, and adaptive strategies relevant to the growing tourism context, based on hourly and monthly climate data from 2014 to 2024. The results show that João Pessoa presents a more stable thermal regime with fewer extreme heat events, yet consistently higher daytime PET values, especially between 9:00 and 15:00, throughout the year. The city also experiences a greater frequency of moderate-to-heavy rainfall during its defined wet season (April to July), often influenced by low-predictability atmospheric systems such as Easterly Wave Disturbances (EWDs). CTIS results confirm high climatic suitability for tourism and recreation during the dry season but reduced suitability during the rainy season. These findings suggest that integrating climate adaptation strategies into tourism planning, such as diversifying attractions beyond sun-and-beach tourism and improving real-time climate communication, may help reduce the impact of seasonal variability on visitor experience
Trop-2 expression in solid tumors : expanded protein diagnostics within the molecular tumor board
No full textHintergrund„Trophoblast surface antigen 2“ (Trop-2) ist ein Oberflächenantigen, das als Zielstruktur neuer Antikörper-Wirkstoff-Konjugate (ADC) dient. Wir berichten über die Evaluation der Trop-2-Expression und die Bestimmung des Biomarkers in soliden Tumoren im Rahmen einer erweiterten Proteindiagnostik im Molekularen Tumorboard Freiburg (MTB).MethodikDie entitätenübergreifende Kohorte umfasst 50 Fälle des Comprehensive Cancer Center Freiburg (CCCF), die im interdisziplinären MTB vorgestellt wurden und für die eine Trop-2-Immunhistochemie (IHC) durchgeführt wurde. Die Trop-2-Expression wurde mittels IHC an formalinfixierten, in Paraffin eingebetteten Gewebeproben bestimmt. Unter Verwendung des H‑Scores wurden die Proben in die Kategorien „negativ“, „niedrige“, „mittlere“ und „hohe“ Expression eingeteilt. Bei 22 Patienten erfolgte zusätzlich eine massenspektrometrische (MS) Trop-2-Analyse sowie eine Korrelationsanalyse der Trop-2-Intensitäten.ErgebnisseDie Trop-2-Expression wurde in 16 % der Fälle als negativ, in 20 % der Fälle niedrig, in 18 % der Fälle mäßig und in 46 % der Fälle als hoch bewertet. Die Trop-2-IHC-Scores korrelieren positiv mit den MS-basierten Proteinintensitäten. Behandlungsempfehlungen für ein Trop-2-ADC wurden in 61 % aller Fälle gegeben und Trop-2-ADC wurden in 20 % der Fälle als therapeutischer Ansatz mit der höchsten Priorität bewertet.DiskussionDie Trop-2-Bestimmung bietet eine Grundlage für personalisierte Behandlungsempfehlungen, die Priorisierung von Therapieoptionen und den Off-Label-Use im MTB. Die positive Korrelation zwischen den Trop-2-ICH-Werten und MS-basierten Intensitäten validiert die IHC und weist auf ein zukünftiges Potenzial einer Multi-Omics-Bewertung therapeutischer Biomarker hin.BackgroundTrophoblast surface antigen 2 (Trop-2) is a surface antigen that can be targeted by novel antibody-drug conjugates (ADCs). Here we report on the systematic evaluation of Trop‑2 expression and the determination of the biomarker in solid tumors as part of expanded protein diagnostics within the molecular tumor board (MTB) in Freiburg.MethodsThe pan-cancer cohort examined comprises 50 patients from the Comprehensive Cancer Center Freiburg who were enrolled in the MTB and additionally received a Trop‑2 IHC staining that was performed using formalin-fixed and paraffin-embedded tissue samples. Utilizing the H‑score, the samples were categorized into negative-, low-, moderate-, or high-expressing tumors. Additionally, mass spectrometry (MS)-based proteomics was performed on 22 tissue samples and used for correlation analysis with IHC expression levels.ResultsTrop‑2 expression was negative in 16%, low in 20%, moderate in 18%, and high in 46% of the assessed cases. The Trop‑2 IHC scores showed a positive correlation with MS-based protein intensities (Pearson correlation coefficient 0.48). Treatment recommendation for Trop‑2 ADC was given in 61% and Trop‑2 ADC was considered as the best therapeutic option in 20%.DiscussionThe evaluation of Trop‑2 expression might be helpful for personalized treatment recommendations in the off-label setting as well as ranking strategies for personalized therapeutic options within the MTB. The positive correlation between Trop‑2 IHC scores and MS-based protein intensities validates the IHC and demonstrates the promise of multi-omics assessment of therapeutic biomarkers
Metallization without charge transfer in CuReO4 perrhenate under pressure
No full textUsing high-pressure synchrotron X-ray diffraction combined with Raman spectroscopy and density-functional calculations, we determined the sequence of the pressure-induced transformations in CuReO4. At 1.5 GPa, the lattice symmetry changes from I41cd to I41/a with the transformation of isolated ReO4-tetrahedra into infinite chains of ReO6-octahedra. The second, isosymmetric transition at 7 GPa leads to the formation of a NbO2-type structure with the octahedral oxygen coordination for both Cu1+ and Re7+ cations. Both transitions are of the first order and accompanied by discontinuities in the unit-cell volume of 7 and 14%, respectively. Density-functional calculations predict the metallic state of the high-pressure NbO2-type phase of CuReO4, and this prediction is in-line with the disappearance of the Raman signal above 7 GPa and visual observations (darkness/reflection of the sample). This metallization is caused by the increased bandwidth of both Cu 3d and Re 5d bands without any significant charge transfer between Cu and Re. At ambient pressure, the crystal structure of CuReO4 is retained between 4 and 700 K (melting point), showing a negative thermal expansion along the c-axis and a positive expansion along the a-axis within the entire temperature range
Prognosis and quality of life in patients with locally advanced rectal cancer after abdominoperineal resection in the CAO/ARO/AIO-04 randomized phase 3 trial
No full textLow anterior resection (LAR) and abdominoperineal resection (APR) are the two main surgical procedures after preoperative chemoradiotherapy (CRT) for locally advanced rectal cancer. APR is associated with poorer prognosis; however existing data do not consider intensified CRT (5-Fluorouracil (5-FU)/Oxaliplatin + radiation) protocols. Clinicopathological data of patients treated with APR and LAR from the CAO/ARO/AIO-04 trial were analysed in terms of prognostic parameters and quality of life (QoL). Based on higher response rate after intensified CRT, subgroup analyses were performed. Data from n = 1173 patients were assessed. APR after preoperative CRT was associated with a significantly worse overall survival (p = 0.0056), disease-free survival (p < 0.0001) and local recurrence rate (p = 0.0047). Clinicopathological data including clinical T stage (p < 0.000001), grading (p = 0.0038), postoperative lymph node (LN) positivity (p = 0.013), and number of positive LN (p = 0.0049) significantly differed between procedures and showed higher values in APR patients. The quality of total mesorectal excision (TME) was significantly better (p < 0.0001) and complete resection rates were higher (p = 0.0022) in LAR compared to APR patients. Subgroup analyses showed worse LR rates in APR patients after standard CRT (5-FU mono and radiation) but not after intensified CRT. After 3 years, role functioning (p = 0.019) and physical functioning (p = 0.001) had a slightly poorer outcome in APR patients. The poorer prognosis of patients undergoing APR for locally advanced rectal cancer may be explained by clinicopathological characteristics. Intensified CRT may compensate for the higher risk of LR after APR in patients with worse TME quality. QoL in APR patients was comparable to LAR patients
