53005 research outputs found
Sort by
The Antimitotic Srug BAY-293 Reduces Acetylation at H3K27 and Decreases Diversity of Genes Expressed while Reducing Expression of rRNA and Ribosomal Proteins in Tetrahymena Thermophila
Get PDFThe methylation and acetylation of histone tails is a critical part of gene regulation which has implications for homeostasis and disease. In cancers such as diffuse intrinsic pontine glioma (DIPG) where many patients are heterozygous for a mutation on histone H3 that replaces lysine with methionine, cells show a higher amount of acetylation and less trimethylation at this position. Tetrahymena thermophila are free-living, eukaryotic ciliates that are often used for epigenetic studies due to their rapid rate of cell division and the ease with which they may be cultured. We have previously shown that BAY-293, an inhibitor of the Ras-GEF Sos, significantly impacts mitotic rate in Tetrahymena thermophila. Since lysine 27 of histone H3 is apparently a critical residue impacting growth regulation, we asked the following questions: 1. Would treatment with BAY-293 impact H3K27ac in Tetrahymena thermophila as measured by immunofluorescence? 2. Would BAY-293 affect gene expression in this organism as measured by total RNA sequencing? Our results indicate that 90-minute exposure to BAY-293 significantly reduces acetylation at H3K27. Acetylation returns to normal once the drug has been washed out for 90 minutes. 90-minute exposure to BAY-293 also reduces a number of cellular RNA populations, including mRNA and rRNA
Sing Sing Sing: Analysis of the Term Sing in the Psalms and Contemporary Worship Music
Get PDFThis paper compares the use of the word SING as translated in the Psalms with SING in contemporary worship music with the intent of identifying similarities and differences in the instruction, expression, and conviction of singing to the Lord.
Three Hebrew terms are primarily translated as SING in the Psalms: rānan רָנַן (rejoice, sing out, shout for joy), zāmar זָמַר (sing with instruments, make music, sing together), and šîr שִׁיר (vocalize, make music with the human voice). When examining the frequency of these Hebrew words, we find SING or a variation thereof in over half of the Psalms. This analysis will also consider how our contemporary culture’s understanding of singing within congregational worship has developed over the decades.
Worship and singing are intrinsically intertwined. Yet, most worship leaders would agree with pastors and theologians that worship is far more than singing. They are not synonymous terms by any means. However, singing is one of the prominent ways to worship God. “Truly worshipful music is primarily congregational and unites the body of Christ.”[1] This is evident throughout scripture. Conclusively, worship is not reduced to singing alone. However, songs of praise to God were “an essential ingredient of worship.”[2] As Thornton concludes, “gathered believers express their relationship with God through the singing of songs.”[3]
This paper is an encouragement to strive to articulate biblically sound, theologically rich, congregationally uniting, Christ-centered, Holy Spirit-inspired, worship to the Father through the songs we sing as a gathered body of believers. May our songs continually teach and encourage us to sing, equally with skill and with abandon, expressing our worship with exuberance and joy.
[1] Daniel I. Block, For the Glory of God: Recovering a Biblical Theology of Worship (Grand Rapids, Michigan: Baker Academic, a division of Baker Publishing Group, 2014), 242.
[2] Ross, Recalling the Hope of Glory, 437.
[3] Thornton, Daniel, “Exploring the Contemporary Congregational Song Genre: Texts, Practice, and Industry,” 47
2024 Men\u27s Cross Country Team
No full textColor photograph of a men\u27s cross country teamhttps://digitalcommons.cedarville.edu/mens_cross_country_gallery/1054/thumbnail.jp
2024-2025 Women\u27s Soccer Team
No full textColor photograph of a women\u27s soccer teamhttps://digitalcommons.cedarville.edu/womens_soccer_gallery/1028/thumbnail.jp
Dr. Lori Ferguson
No full text2023-2024 recipienthttps://digitalcommons.cedarville.edu/faculty_innovator/1019/thumbnail.jp
Hospitality Amid Grief in 19th-century Cedarville, Ohio
Get PDFThis essay explains the history of child mortality in 19th-century Cedarville, Ohio and connects it to Martha McMillan and journals. Florence, Martha’s daughter, died in 1888, and Martha records her personal grief from Florence’s death and the deaths of other children in the community in 1889. Martha’s 1889 journal records the incredible hospitality that shaped her community during times of grief from child moralities
Class Picture
No full texthttps://digitalcommons.cedarville.edu/white_coat_ceremony_gallery_2024/1004/thumbnail.jp
