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Novel probiotic lactic acid bacteria isolated from indigenous fermented foods from West Sumatera, Indonesia
Get PDFBackground and Aim: Probiotics play an important role in maintaining a healthy gut and consequently promote good health. This study aimed to find novel probiotic lactic acid bacteria (LAB) from indigenous fermented foods of West Sumatera, Indonesia. Materials and Methods: This study utilized 10 LAB previously isolated from fermented buffalo milk (dadih), fermented fish (budu), and fermented cassava (tape) which have the ability to produce gamma-aminobutyric acid. The study commenced with the screening of LAB for certain properties, such as resistance to acid and bile salts, adhesion to mucosal surface, and antagonism against enteric pathogens (Escherichia coli, Salmonella Enteritidis, and Staphylococcus aureus). The promising isolates were identified through biochemical and gram staining methods. Results: All isolates in this study were potential novel probiotics. They survived at a pH level of 2.5 for 3 h (55.27-98.18%) and 6 h (50.98-84.91%). Survival in bile at a concentration of 0.3% was 39.90-58.61% and the survival rate was 28.38- 52.11% at a concentration of 0.5%. The inhibitory diameter ranged from 8.75 to 11.54 mm for E. coli, 7.02 to 13.42 mm for S. aureus, and 12.49 to 19.00 mm for S. Enteritidis. All the isolates (84.5-92%) exhibited the ability to adhere to mucosal
surfaces. This study revealed that all the isolates were potential probiotics but N16 proved to be superior because it was viable at a pH level of 2 (84.91%) and it had a good survival rate in bile salts assay (55.07%). This isolate was identified as Lactobacillus spp., Gram-positive bacilli bacteria, and tested negative in both the catalase and oxidase tests. Conclusion: All the isolates in this study may be used as probiotics, with isolate N16 (Lactobacillus spp.) as the most promising novel probiotic for poultry applications based on its ability to inhibit pathogenic bacteria
International Journal of Enviroment, Agriculture and Biotechnology, 5(4) edtion
Get PDFAbstract— Patchouli (Pogostemon cablin Benth) is a major essential oil-producing plant in Indonesia. But
its productivity is still low that influenced by low genetic quality. In West Pasaman Regency, there are
three patchouli local accessions namely Situak, Rimbo Binuang, and Tombang that have potential as a
genetic resource in the breeding program. In this study, the tissue culture method was used for plant
propagation to produce superior seedling in a relatively short time. This study aims to determine the
effective concentration of BA for in vitro callus formation and plantlet regeneration in three patchouli
accessions. The method used is Random Design (CRD) with 2 factors, namely the concentration of BA
(0.01 and 0.03 ppm) and plant accessions (Situak, Rimbo Binuang, and Tombang), with 6 total treatments
using 5 replications. Data were analyzed statistically by the F test at a 5% significance level and followed
by the DNMRT at a 5% level. The results showed that the treatment of BA 0.01 ppm in Rimbo Binuang
accession produce the highest value in all variables which are live explants percentage, explants forming
callus percentage, number of shoots per explant, number of total leaves, and number of leaves per shoot
are 92 %, 92 %, 5.30 shoots, 28.80 leaves, and 4.20 leaves respectively. So, it concluded that the 0.01 ppm
BA is an optimal concentration as a patchouli in-vitro growth inducer. The best plant accession as a
genetic source in plant propagation using BA is the Rimbo Binuang accession.
Keywords— aromatherapy, cytokinin, essential oil, organogenesis, perfume
Kajian Fitokimia Fraksi Etil Asetat dari Lichen Stereocaulon massartianum Hue. dan Uji Aktivitas Antibakteri dengan Metode KLT-Bioautografi
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Cross-culture communication
No full textA medical faculty student must be able to communicate with patients from various backgrounds, including various ethnic groups. One ethnic group that has specific principles and rules in communicating is the Minangkabau tribe. Medical students who are studying at the Faculty of Medicine of Andalas University are important to understand the principles of communicating according to Minangkabau cultur