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Cell-to-cell variability in the yeast pheromone response: Cytoplasmic microtubule function stabilizes signal generation and promotes accurate fate choice
Full text linkIn a companion paper, we carried out a high-throughput screen to identify genes that suppressed cell-to-cell variability in signaling in yeast. Two genes affected cytoplasmic microtubules that can connect the nucleus to a signaling site on the membrane. Here, we show that microtubule perturbations that affected polymerization and depolymerization, membrane attachment, and force generation increased variability. For some perturbations, "outlier" cells drove the increased variability. Bypass experiments that activated the PRS ectopically at downstream points indicated that microtubule-dependent processes might stabilize the membrane-recruited scaffold protein Ste5. The variability caused by microtubule perturbations required the MAP kinase Fus3. Microtubule perturbations hindered stable scaffold formation and decreased the accuracy of a polarity-dependent fate choice. Our experiments suggest that membrane-attached microtubules stabilize signaling by scaffold-bound Fus3, and are consistent with a model in which signaling irregularities from changes in microtubule function are amplified by cross-stimulatory feedbacks among PRS proteins. The fact that microtubule perturbations also cause aberrant fate and polarity decisions during embryonic development and cancer initiation suggests that similar variation-reducing processes might also operate in metazoans
No Bad Slides: Scientific Presentations for All Audiences
No full textOver the last several decades several factors have produced a communication crisis in biomedical research. The field has grown larger, specialization has increased, there are more non-native English speakers, wireless technology diverts audiences, and easy-to-use presentation software has led to slide proliferation. As a result, scientists spend increasing amounts of time in incomprehensible presentations.
This talk will provide an overarching strategy for developing a scientific presentation. The talk will outline methods for developing a compelling narrative and converting that narrative into visually effective slides that articulate the essential technical message. It will review strategies for adapting talks to audiences with different levels of expertise.
Speaker Bio: David Rubenson is the Administrative Director of the Stanford Cancer Institute since 2009. He also spent 7 years as Director of Special Projects at City of Hope where he helped physicians and scientists develop presentations. This talk was presented on April 14, 2016 at the Fred Hutchinson Cancer Research Center
