81690 research outputs found
Sort by
ヤングケアラーと家族の貧困 [全文の要約]
No full textこの博士論文全文の閲覧方法については、以下のサイトをご参照ください。https://www.lib.hokudai.ac.jp/dissertations/copy-guides
オキシリピンおよび短鎖脂肪酸の高感度LC/MS分析法の開発とその応用
No full textOxylipins are produced from polyunsaturated fatty acids (PUFAs) and short-chain fatty acids (SCFAs) formed through microbial fermentation. They are essential metabolites with diverse physiological roles, supporting metabolic health and development in children. This study aims first to develop a sensitive and selective analytical method, followed by a systematic evaluation of oxylipin, PUFA, and SCFA concentrations in the plasma of children, exploring associations with factors such as age, sex, and body mass index (BMI).
In this study, a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the quantification of 64 oxylipins and 4 precursor PUFAs within 36 minutes. Most oxylipins exhibited recoveries ranging from 85% to 110%, with excellent precision (intra- and inter-day variability within 15%). This method was applied to plasma samples from 342 children born between 2006 and 2010 (181 boys and 161 girls). Results showed no significant correlations between oxylipin levels and age, sex, or BMI; however, among PUFA levels, boys exhibited higher eicosapentaenoic acid and arachidonic acid concentrations compared to girls, and overweight children had significantly higher eicosapentaenoic acid levels than underweight children.
Additionally, SCFA analysis in the same plasma samples was conducted using a previously developed LC-MS/MS method with N,N-dimethylethylenediamine (DMED) derivatization, achieving recoveries of 83% to 108% with ethyl acetate extraction. Results indicated that acetic acid was the most abundant SCFA across all age groups and sexes. Concentrations of butyric acid, valeric acid, and hexanoic acid increased with age, whereas 4-hydroxy valeric acid levels declined. Additionally, overweight children displayed relatively higher propionic acid concentrations.
In conclusion, a high-sensitivity and high-selectivity analytical method for oxylipins was successfully developed and optimized, and the distributions of oxylipins, PUFAs, and SCFAs in plasma from children were systematically quantified. This method provides a robust foundation for further studies and supports its broader application in diverse biological samples, while offering scientific insights to inform nutritional interventions and health promotion strategies targeting child health
