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ポドプラニンコンディショナルノックアウトマウスにおける骨細胞の形態学的研究
No full textWe generated podoplanin-conditional knockout mice where the floxed podoplanin exon3 was deleted by the Dmp-1-driven Cre (Dmp1-Cre;PdpnΔ/Δ) and investigated the cell process elongation of podoplanin-deficient mouse osteocyte in vitro and in vivo. The expression of podoplanin is found in odontoblasts while not observed in odontoblasts of Dmp1-Cre;PdpnΔ/Δ mice, indicating that the conditional knockout of podoplanin in Dmp1-expressing cells in Dmp1-Cre;PdpnΔ/Δ mice is successful. There were no differences in the growth of wild-type and Dmp1-Cre;PdpnΔ/Δ mice, and no differences in calci-fication and alkaline phosphatase activity in cultured calvarial osteoblasts of the wild-type and Dmp1-Cre;PdpnΔ/Δ mice,in total this suggests that the podoplanin-cKO has no effect on generation of the bone. The cell process elongation was sup-pressed in cultured calvarial osteoblasts of Dmp1-Cre;PdpnΔ/Δ mice compared with wild-type mice. In the electron micro-scopic study, there were no morphological differences in bone matrix formation and osteocyte distribution in Dmp1-Cre;PdpnΔ/Δ and wild-type mice, whereas the cell process formation was sparser and the network with neighboring cells was more deficient in Dmp1-Cre;PdpnΔ/Δ mice than in wild-type mice. In the quantitative analysis, the number and thickness of the cell processes were significantly smaller and thinner in Dmp1-Cre;PdpnΔ/Δ mice than in wild-type mice. This could sug-gest that podoplanin plays a role in the formation of the osteocyte network created by the cell process elongation
チョウザメ類卵濾胞における排卵能獲得誘導の分子機構に関する研究 [全文の要約]
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ウロン酸修飾を活用した魚肉タンパク質の抗炎症機能改変に関する研究 [全文の要約]
No full textこの博士論文全文の閲覧方法については、以下のサイトをご参照ください。https://www.lib.hokudai.ac.jp/dissertations/copy-guides
