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Tissue engineered corneal epithelium derived from clinical-grade human embryonic stem cells
Get PDF角膜上皮位于角膜最表层，对维持角膜的透明性发挥非常重要的作用。角膜上皮细胞处于不断更新之中，它们更新的源泉在于角膜缘处的上皮干细胞，即角膜缘干细胞。眼表面化学伤、热烧伤以及部分先天性遗传疾病可以引起角膜缘干细胞缺乏，或者使干细胞丧失向正常角膜上皮细胞分化的能力，导致经久不愈的角膜上皮缺损、角膜新生血管、角膜上皮结膜化、角膜溃疡等病理改变，严重影响患者视力，甚至导致失明。由于眼表面的严重破坏，这类患者的治疗具有很大的挑战性，目前比较理想的治疗方法是进行组织工程角膜上皮移植。
厦门大学眼科研究所和中国科学院动物研究所干细胞与生殖生物学国家重点实验室合作研究采用中科院动物研究所研发的我国第一个临床级胚胎干细胞系Q-CTS-hESC-1，体外诱导分化为角膜上皮祖细胞，在此基础上构建组织工程角膜上皮，移植于兔角膜缘干细胞缺乏动物模型，成功重建眼表面。
厦门大学眼科研究所博士研究生贺佳和欧尚坤为该论文的共同第一作者，厦门大学眼科研究所李炜教授、刘祖国教授以及中科院动物所郝捷副研究员为共同通讯作者。【Abstract】Purpose: To construct tissue engineered corneal epithelium from a clinical-grade human embryonic stem cells (hESCs) and investigate the dynamic gene profile and phenotypic transition in the process of differentiation.Methods: A stepwise protocol was applied to induce differentiation of clinical-grade hESCs Q-CTS-hESC-1 and construct tissue engineered corneal epithelium. Single cell RNA sequencing (scRNA-seq) analysis was performed to monitor gene expression and phenotypic changes at different differentiation stages. Immunostaining, realtime quantitative PCR and Western blot analysis were conducted to detect gene and protein expressions. After subcutaneous transplantation into nude mice to test the biosafety, the epithelial construct was transplanted in a rabbit corneal limbal stem cell deficiency (LSCD) model and followed up for eight weeks.
Results: The hESCs were successfully induced into epithelial cells. scRNA-seq analysis revealed upregulation of ocular surface epithelial cell lineage related genes such as TP63, Pax6, KRT14, and activation of Wnt, Notch,Hippo, and Hedgehog signaling pathways during the differentiation process. Tissue engineered epithelial cell sheet derived from hESCs showed stratified structure and normal corneal epithelial phenotype with presence of clonogenic progenitor cells. Eight weeks after grafting the cell sheet onto the ocular surface of LSCD rabbit model, a full-thickness continuous corneal epithelium developed to fully cover the damaged areas with normal limbal and corneal epithelial phenotype.
Conclusion: The tissue engineered corneal epithelium generated from a clinical-grade hESCs may be feasible in the treatment of limbal stem cell deficiency.This study was supported in part by the National Key R&D Program of China (2018YFA0107301 [to WL], 2018YFA0107304 [to ZL], 2013CB967003 [to WL]), the National Natural Science Foundation of China (NSFC, No.81770894, No.81470602 [to WL], and No.81330022 [to ZL]). 该论文获得了国家重点研发计划项目和国家自然科学基金项目的资助
Regional Cooperation and the National Business Environment:Based on a Study of the China-ASEAN Collaboration on the BRI
No full text李一平，厦门大学东南亚研究中心/“一带一路”研究院教授、博士生导师，历史学博士；罗晶晶，厦门大学东南亚研究中心/“一带一路”研究院博士研究生；张海峰，南京财经大学财政与税务学院讲师，经济学博士。【中文摘要】作为国际合作2.0升级版的“一带一路”倡议，对沿线国家经济发展产生何种影响是非常值得研究的前沿话题。从国家营商环境改善角度，以世界银行公布的数据为基础对东盟十国营商环境进行分析，首次利用双重差分法研究共建“一带一路”对沿线东盟十国营商环境的影响可以发现，共建“一带一路”对改善沿线国家营商环境具有显著促进作用，且共建力度越强、对华经贸 合作规模越大的国家，其营商环境提升越明显。研究结论有助于国家和企业明晰投资区位，增加企业和市场主体对“一带一路”沿线国家的投资意愿和动力，也为实现沿线国家间投资贸易便利化,实现“ 一带一路”利益共同体的战略目标提供理论支持。
【Abstract】The impact of the Belt and Road Initiative (BRI) on the economic development of the participant countries in Southeast Asia is a new frontier of research. Utilizing the World Bank's data，this article examines the contribution of the BRI to the improvement of the business environment in ASEAN countries. To the best knowledge of the author，this is the first project that has employed the Differences-in-Differences method to analyze the impact of BRI collaboration on the business environment of the ten ASEAN countries. The study has found that BRI collaboration has significantly improved the business environment of the countries along the route. Closer collaborative efforts and broader economic cooperation with China mean greater improvements in the business environment of these countries. The article finds the BRI has helped enterprises identify investment locations and increased the willingness and motivation of enterprises and economic entities to invest in the countries along the BRI. Furthermore，this paper provides theoretical guidelines and empirical evidence to reduce investment and trade barriers among the countries along the BRI as well as to build a community with a shared future for mankind.福建省教育厅“一带一路”与东南亚研究院智库项目
Porous Electrodes in Electrochemical Energy Storage Systems
No full text查全性教授是中国现代电化学的奠基人之一. 在他的带领下,武汉大学化学系电化学研究室在基础电化学和应用电化学领域取得了卓越的成绩. 查教授及同事们在过去几十年里,栽培学生无数. 后来,一部分学生有幸成为推动世界电化学学科发展的中坚力量. 在这篇综述中,作者将概述查教授及同事们在电化学领域打下的夯实基础,及作者在多孔电极方向的研究进展. 本文的所有作者均于不同时期毕业于武汉大学. 站在巨人的肩膀上,我们实属荣幸！Professor C.S. Cha was among the pioneers who have introduced modern electrochemistry to China. Under his leadership, the electrochemical research group in Wuhan University became one of the global powerhouses in fundamental and applied electrochemical researches. During the past many decades, Professor Cha and his colleagues in the university have educated and trained many students who have become part of the backbone of electrochemistry worldwide. In this review, we demonstrate the solid foundation laid by Professor Cha and his colleagues in Wuhan University, and the advancements made in the area of porous electrodes by the authors. All the authors in this paper graduated from Wuhan University at different times. We are fortunate to be standing on the shoulders of a giant.通讯作者:屈德扬E-mail:[email protected]:QUDe-yangE-mail:[email protected].威斯康辛大学密尔沃基分校工程与应用科学学院机械工程系,威斯康辛州,53211,美国2.武汉大学化学与分子科学学院,武汉,430072,中国3.爱达荷州国家实验室,爱达荷州,83401,美国1. Department of Mechanical Engineering, College of Engineering and Applied Science, University of Wisconsin Milwaukee, Milwaukee, WI, 53211, USA2. Department of Chemistry, Wuhan University, Wuhan, 430072, P.R. China3. Idaho National Laboratory, 775 MK Simpson Blvd. Idaho Fall, ID 8340
Synergistic Effect of Dissolving O2 and Wavelength on the Photo-Assisted Anodic Deposition of CeO2 Thin Films
No full text国家自然科学基金项目资助No(51771173);国家自然科学基金项目资助No(21171155);国家自然科学基金项目资助No(51471156)通讯作者:杨雨萌,卫国英E-mail:[email protected];[email protected]:YANGYu-meng,WEIGuo-yingE-mail:[email protected];[email protected]. 中国计量大学材料与化学学院,杭州 3100182. 浙江大学化学系,杭州 3100271. School of Materials and Chemistry, China Jiliang University, Hangzhou, 310018, China2. Department of Chemistry, Zhejiang University, Hangzhou, 310027, Chin
Effect of Reaction Conditions on Cu⁃Catalyzed CO2 Electroreduction
Get PDF工业规模的化石能源消耗导致大气中二氧化碳含量不断增加,CO2转化利用成为人们日益关注的热点问题. 金属铜因其成本低廉、储量丰富,并且具有独特的CO2亲和力能够生成多碳化合物,是目前CO2电还原中研究最为广泛深入的电极材料. 由于阴、阳离子的特征吸附对Cu电极性能有显著影响,并且不同反应体系中对Cu电极上CO2吸附、活化影响也有所不同,因此导致金属Cu电极上报道的电催化活性、产物种类与选择性等都非常宽泛. 基于此,有必要系统地研究各种反应条件对金属Cu电极电催化CO2还原性能的影响. 作者选择了平均粒径为600 nm的商品化金属Cu颗粒作为电还原CO2的催化剂,研究了不同反应条件包括各种常用电解质溶液、KHCO3的浓度以及H型电解池和流动池. 实验结果表明,浓度为0.5 mol·L -1的KHCO3作为电解质溶液具有较好催化活性和较高的产物分电流密度,流动池可以进一步提高主要产物甲酸盐和CO的分电流密度. 本研究工作从反应条件的角度对CO2还原的电催化转化进行了系统研究,有助于理解电解液和反应器等因素对CO2电还原反应过程的影响规律.通讯作者:陈为,魏伟,孙予罕E-mail:[email protected];[email protected];[email protected]:CHENWei,WEIWei,SUNYu-hanE-mail:[email protected];[email protected];[email protected]. 中国科学院上海高等研究院，中国科学院低碳转化科学与工程重点实验室，上海 2012102. 中国科学院大学，北京 1000493. 上海科技大学物质科学与技术学院，上海 2012101. CAS Key Laboratory of Low-carbon Conversion Science and Engineering, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai, 201210, China2. University of Chinese Academy of Sciences, Beijing, 100049, China3. School of Physical Science and Technology, ShanghaiTech University, Shanghai, 201210, Chin
Virus-Free and Live-Cell Visualizing SARS-CoV-2 Cell Entry for Studies of Neutralizing Antibodies and Compound Inhibitors
Get PDF新型冠状病毒SARS-CoV-2在全球蔓延，给全球公共卫生带来严重威胁。快速研制疫苗、抗体和治疗药物成为科学界面临的重大挑战。由于SARS-CoV-2的高度传染性，采用病毒感染模型进行中和抗体及小分子抑制剂的药效评估需要在高等级生物安全实验室中进行，且常需要数天时间才能完成检测，限制了抗体和药物筛选的效率。发展快速、可视、不依赖于活病毒的新冠病毒入胞检测探针和细胞模型，对于加速新冠病毒抗体和药物的研究有重要意义。夏宁邵教授团队通过CHO真核表达系统高效表达制备出C端融合抗酸荧光蛋白Gamillus的重组新冠病毒spike蛋白STG。STG经SEC分子筛和冷冻电镜确认呈现与天然病毒刺突高度相似的三聚体结构，且与ACE2有很高的亲和力（18.2nM）。STG具备良好的细胞相容性和荧光性质，研究者进一步开发了可定量测定感染恢复期血清、疫苗免疫血清中和抗体（入胞阻断抗体）水平的CSBT检测方法。除了抗体检测评估方面的应用外，该研究发展的探针和模型还可用于筛选分析抑制新冠病毒入胞及胞内转运的小分子化合物。
我校博士后张雅丽，博士生王邵娟、巫洋涛，博士后侯汪衡、袁伦志和深圳市第三人民医院沈晨光博士为共同第一作者。厦门大学夏宁邵教授、袁权教授、程通教授为该论文共同通讯作者。The ongoing corona virus disease 2019 (COVID-19) pandemic, caused by SARS-CoV-2 infection, has resulted in hundreds of thousands of deaths. Cellular entry of SARS-CoV-2, which is mediated by the viral spike protein and
ACE2 receptor, is an essential target for the development of vaccines, therapeutic antibodies, and drugs. Using a mammalian cell expression system,a genetically engineered sensor of fluorescent protein (Gamillus)-fused
SARS-CoV-2 spike trimer (STG) to probe the viral entry process is developed.In ACE2-expressing cells, it is found that the STG probe has excellent performance in the live-cell visualization of receptor binding, cellular uptake, and intracellular trafficking of SARS-CoV-2 under virus-free conditions. The new system allows quantitative analyses of the inhibition potentials and detailed influence of COVID-19-convalescent human plasmas, neutralizing antibodies and compounds, providing a versatile tool for high-throughput screening and phenotypic characterization of SARS-CoV-2 entry inhibitors. This approach may also be adapted to develop a viral entry visualization system for other viruses.This study was supported by National Natural Science Foundation of China (81993149041 for N.X.; 81902057 for Y.Z.; 81871316 and U1905205 for Q.Y.), the National Science and Technology Major Project of Infectious Diseases (No. 2017ZX10304402‐002‐003 for T.C. and No. 2017ZX10202203‐009 for Q.Y.), the National Science and Technology Major Projects for Major New Drugs Innovation and Development (No. 2018ZX09711003‐005‐003 for T.C.), the Science and Technology Major Project of Fujian (2020YZ014001), the Science and Technology Major Project of Xiamen (3502Z2020YJ01), and the Guangdong Basic and Applied Basic Research Foundation (2020A1515010368 for C.S.).
该研究得到了国家自然科学基金、传染病防治国家科技重大专项、福建省应急科技攻关项目和厦门应急科技攻关项目的支持
Dynamics of Ammonium Biogeochemistry in an Oligotrophic Regime in the South China Sea
No full textAmmonium (NH4+) dynamics in the oligotrophic South China Sea (SCS) was examined via a large data set developed during summer 2014 and spring 2016. A sensitive fluorometric technique allowed measuring NH4+ concentrations down to 0.7 nmol L-1. The NH4+ inventory of the upper 100 m showed large inter-annual variation, averaging 3.46 mmol m-2 in 2014 and almost doubling in 2016. This could be attributed to seasonality or more likely to influence of Kuroshio Current intrusion, prominent within El Niño years, as in 2016. Indeed, Kuroshio-influenced stations, yielded elevated NH4+ and lower NO3- inventories. Higher NH4+ levels at the Kuroshio-SCS frontal zone were consistent with prior observations of enhanced microbial consumption of Kuroshio-derived dissolved organic nitrogen. Two patterns in vertical NH4+ distributions were observed: a subsurface NH4+ maximum (AM; 30.1-241 nmol L-1), usually occurring at 50-100 m and closely coupled with the deep chlorophyll maximum, and one where NH4+ concentrations were low and generally vertically uniform. Dynamics of the AM appears to be controlled by the euphotic depth, position of the nitracline, as well as the difference in the affinity to NH4+ between phytoplankton and nitrifiers. Results demonstrate that precise measurements of NH4+ concentrations at nanomolar levels facilitate improved understanding of coastal nitrogen biogeochemistry.Ministry of Science and Technology of the People's Republic of China (MOST): 2015CB954003; National Natural Science Foundation of China (NSFC): 41606089; National Natural Science Foundation of China (NSFC): 9185810
Recent Progress in the Mechanistic Understanding of CO2 Reduction on Copper
No full text本文从历史角度综述了二氧化碳在铜基催化剂上的还原机理的最新研究进展，对区分C1和C2产物路径发生的机制，以及调控二氧化碳还原产物选择性的影响因素和方法进行了重点阐述，着重讨论了如何利用电化学红外光谱与微分电化学质谱等技术在揭示反应机理方面的研究思路与方法学.In this review, we present the major developments in the understanding of the mechanisms of the electrochemical reduction of CO2 from a historical perspective. Most of the work discussed in this review was carried out at copper electrodes, as this is the only material at which hydrocarbons are produced in reasonable quantities. The emphasis focuses on the differentiation of mechanisms for the generation of C1 and C2 products as well as factors and methods for controlling the product selectivity of CO2 reduction. We have highlighted ambiguities, assumptions, and important methodologies, such as differential electrochemical mass spectrometry and electrochemical in-situ infrared spectroscopy, which help greatly to clarify these issues in the literature.This work was supported by the Natural Science Foundation of China(No. 91545124, No. 21750110437). MMS is supported by the Chinese Academy of Sciences President’s International Fellowship Initiative (Grant No. 2017PM0049)作者联系地址：合肥微尺度物质科学国家研究中心，中国科技大学化学物理系，安徽 合肥 230026Author's Address: Hefei National Laboratory for Physical Sciences at the Microscale, Department of Chemical Physics, University of Science and Technology of China, Hefei, 230026, China通讯作者E-mail：[email protected]
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No full text作者联系地址：厦门大学化学化工学院Author's Address: College of Chemistry and Chemical Engineering, Xiamen University通讯作者E-mail：[email protected]