41 research outputs found
Cell type–specific cytonuclear coevolution in three allopolyploid plant species
Get PDFCytonuclear disruption may accompany allopolyploid evolution as a consequence of the merger of different nuclear genomes in a cellular environment having only one set of progenitor organellar genomes. One path to reconcile potential cytonuclear mismatch is biased expression for maternal gene duplicates (homoeologs) encoding proteins that target to plastids and/or mitochondria. Assessment of this transcriptional form of cytonuclear coevolution at the level of individual cells or cell types remains unexplored. Using single-cell (sc-) and single-nucleus (sn-) RNAseq data from eight tissues in three allopolyploid species, we characterized cell type–specific variations of cytonuclear coevolutionary homoeologous expression and demonstrated the temporal dynamics of expression patterns across development stages during cotton fiber development. Our results provide unique insights into transcriptional cytonuclear coevolution in plant allopolyploids at the single-cell level.This article is published as Zhang, Keren, Xueru Zhao, Yue Zhao, Zhibin Zhang, Zhijian Liu, Ziyu Liu, Yanan Yu et al. "Cell type–specific cytonuclear coevolution in three allopolyploid plant species." Proceedings of the National Academy of Sciences 120, no. 40 (2023): e2310881120. doi:10.1073/pnas.2310881120. Copyright © 2023 the Author(s). Posted with permission.This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND)
Hybrid dynamic iterations for the solution of initial value problems
No full textManyscientific problems are posed as Ordinary Differential Equations (ODEs). A large subset of these are initial value problems, which are typically solved numerically. The solution starts by using a known state-space of the ODE system to determine the state at a subsequent point in time. This process is repeated several times. When the computational demand is high due to large state space, parallel computers can be used efficiently to reduce the time to solution. Conventional parallelization strategies distribute the state space of the problem amongst cores and distribute the task of computing for a single time step amongst the cores. They are not effective when the computational problems have fine granularity, for example, when the state space is relatively small and the computational effort arises largely from the long time span of the initial value problem. We propose a hybrid dynamic iterations method1 which combines conventional sequential ODE solvers with dynamic iterations to parallelize the time domain. Empirical results demonstrate a factor of two to four improvement in performance of the hybrid dynamic iterations method over a conventional ODE solver on an 8 core processor. Compared to Picard iterations (also parallelized in the time domain), the proposed method shows better convergence and speedup results when high accuracy is required.Journal ArticlePre-prin
Structural damage identification based on moving load and time-frequency entropy analysis
No full textIndole derivatives inhibited the formation of bacterial biofilm and modulated Ca2+ efflux in diatom
No full textMarine biofouling is a serious environmental problem worldwide. As an effort to find environmental friendly antifoulants, indole derivatives were determined for their activities to inhibit the growth of bacteria and diatom. The minimum inhibitory concentrations (MICs) of indole derivatives against bacteria were very low, especially for 6-chloroindole. It was proved that 6-chloroindole obviously inhibited the growth of bacteria, interfered with the formation of bacterial biofilm, destroyed bacterial cell morphology and also inhibited the growth of diatom Cylindrotheca sp. as well. By using noninvasive micro-test technique (NMT), 6-chloroindole triggered algal cellular Ca2+ efflux. The highest value was 72.03 pmol cm(-2) s(-1), 10.6 times of the control group. The present studies indicated that indole derivatives might have the potential to be new antifouling agents because of their excellent antibacterial and anti-algal activities. At the same time, Ca2+ efflux might be one of the mechanisms that indole derivatives inhibited the growth of diatom. (C) 2014 Elsevier Ltd. All rights reserved
A Bibliometric and Scientific Knowledge Map Study of Migraine Treatment from 2013 to 2022
No full textTao Wang,1,2,* Yanan Li,2,* Shuai Miao,1,2 Chunxiao Yang,3 Wei Xie,1,2 Huijuan Yuan,3 Wenhao Bai,1,2 Han Xiao,1,2 Shengyuan Yu1,2 1Department of Neurology, the First Medical Centre, Chinese PLA General Hospital, Beijing, People’s Republic of China; 2Medical School of Chinese PLA, Beijing, People’s Republic of China; 3College of Medicine, Nankai University, Tianjin, People’s Republic of China*These authors contributed equally to this workCorrespondence: Shengyuan Yu, Email [email protected]: Migraine treatment research has made much great progress over the past decade. However, there have been few bibliometric studies conducted so far. In this study, bibliometric analysis was used to explore the current status and future trends of migraine treatment research.Methods: Migraine treatment-related articles were retrieved from the Web of Science Core Collection on December 7, 2022. Quantitative variables were analyzed by the R-tool bibliometrix and Excel 2020. VOS viewer and CiteSpace software were used to visualize citation, co-authorship, co-occurrence, and co-citation analysis of countries/regions, organizations, authors, references, and keywords.Results: A total of 3294 articles were included with the global publication output showing a slow upward trend. The United States was the most productive country with 1116 papers and gained the most citations. Albert Einstein College of Medicine was the most active institution with 176 papers. Headache published the most articles in this domain, while Cephalalgia was the most commonly co-cited journal. Lipton, RB published the most articles and had the most citations. Tepper S, 2017, Lancet neurology and Silberstein S, 2004, Cephalalgia were defined as classic articles. The current research mainly focuses on CGRP-related therapeutics, such as fremanezumab, erenumab and ubrogepant.Conclusion: Based on the analysis of bibliometric data on migraine treatment over the past decade, the trends and the knowledge graph of the country, organization, author, reference, and the keyword were identified, providing accurate and quick positioning of the critical information in the domain.Keywords: migraine, treatment, bibliometric analysis, research trends, hot spot
Research and Application of Three-Dimensional and Three-Phase Nonlinear Flow Numerical Simulator in Ultra-low Permeability Reservoir
No full textImpact of service recovery quality on consumers' repurchase intention: The moderating effect of customer relationship quality
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3-D hand & eye-vergence approaching visual servoing with Lyapunouv-stable pose tracking
No full textHuman CYP2A13 and CYP2F1 Mediate Naphthalene Toxicity in the Lung and Nasal Mucosa of CYP2A13/2F1-Humanized Mice
Get PDFBACKGROUND: The potential carcinogenicity of naphthalene (NA), a ubiquitous environmental pollutant, in human respiratory tract is a subject of intense debate. Chief among the uncertainties in risk assessment for NA is whether human lung CYP2A13 and CYP2F1 can mediate NA's respiratory tract toxicity.
OBJECTIVES: We aimed to assess the in vivo function of CYP2A13 and CYP2F1 in NA bioactivation and NA-induced respiratory tract toxicity in mouse models.
METHODS: Rates of microsomal NA bioactivation and the effects of an anti-CYP2A antibody were determined for lung and nasal olfactory mucosa (OM) from Cyp2abfgs-null, CYP2A13-humanized, and CYP2A13/2F1-humanized mice. The extent of NA respiratory toxicity was compared among wild-type, Cyp2abfgs-null, and CYP2A13/2F1-humanized mice following inhalation exposure at an occupationally relevant dose (10 ppm for 4 hr).
RESULTS: In vitro studies indicated that the NA bioactivation activities in OM and lung of the CYP2A13/2F1-humanized mice were primarily contributed by, respectively, CYP2A13 and CYP2F1. CYP2A13/2F1-humanized mice showed greater sensitivity to NA than Cyp2abfgs-null mice, with greater depletion of nonprotein sulfhydryl and occurrence of cytotoxicity (observable by routine histology) in the OM, at 2 or 20 hr after termination of NA exposure, in humanized mice. Focal, rather than gross, lung toxicity was observed in Cyp2abfgs-null and CYP2A13/2F1-humanized mice; however, the extent of NA-induced lung injury (shown as volume fraction of damaged cells) was significantly greater in the terminal bronchioles of CYP2A13/2F1-humanized mice than in Cyp2abfgs-null mice.
CONCLUSION: CYP2F1 is an active enzyme. Both CYP2A13 and CYP2F1 are active toward NA in the CYP2A13/2F1-humanized mice, where they play significant roles in NA-induced respiratory tract toxicity. https://doi.org/10.1289/EHP844
