110 research outputs found
Research of teaching-learning-doing integrative pattern based on modularization
No full textAiming at the state of independent about the theory teaching and practic teaching in professional education, based on the analysis of employment demand, author present the viewpoint on creating three levels training modules with modular design concept, establishing a system of the teaching in theory and pratic, seting up theory and training integrated pattern of teaching, which was applicated in curriculum of Technology and application on database in good effect. ? 2010 IEEE.EI
RETRACTED ARTICLE: Silence of lncRNA GAS5 alleviates high glucose toxicity to human renal tubular epithelial HK-2 cells through regulation of miR-27a
No full textWe, the Publisher and Editor of Artificial Cells, Nanomedicine, and Biotechnology, have retracted the following article:Lina Lv, Dandan Li, Fengqun Tian, Xia Li, Jing Zhang & Xiulian Yu (2019) Silence of lncRNA GAS5 alleviates high glucose toxicity to human renal tubular epithelial HK-2 cells through regulation of miR-27a. Artificial Cells, Nanomedicine, and Biotechnology, 47(1), 2205–2212, DOI: 10.1080/21691401.2019.1616552After publication of this article, questions about the scientific integrity of the article content were brought to the Publisher and Editor’s attention. We reached out to the authors requesting that they supply information that would confirm the article’s integrity. The authors provided several files in response to our queries, but they were unable to provide the original data in a format which satisfies our requirements to confirm the authenticity of some types of data. In particular, they were unable to provide full western blots, where the molecular weight markers are indicated. It has also come to our attention that the authorship list for this manuscript was changed after the article was accepted. We have contacted the corresponding author for an explanation, but we have not received a response. As determining authorship is core to the integrity of published work, and the authors have also not been able to verify the integrity of their data, we are retracting the article. The corresponding author listed in this publication has been informed.We have been informed in our decision-making by our policy on publishing ethics and integrity and the COPE guidelines on retractions.The retracted article will remain online to maintain the scholarly record, but it will be digitally watermarked on each page as “Retracted”
Effects of warming on ectomycorrhizal colonization and nitrogen nutrition of Picea asperata seedlings grown in two contrasting forest ecosystems
No full textEctomycorrhiza (ECM) plays an important role in plant nitrogen (N) nutrition and regulates plant responded to climate warming. We conducted a field experiment in a natural forest and a plantation in the eastern Tibetan Plateau to estimate the warming effects of open-top chambers (OTC) on ECM and N nutrition of Picea asperata seedlings. Four-year warming significantly decreased ECM colonization, ECM fungal biomass, fine root vigor, and the N concentration of leaf, stem and coarse root, but significantly increased fine root N concentration and N content of leaf, stem, fine root and whole plant in natural forest. Contrarily, warming induced no obvious change in most of these parameters in plantation. Moreover, warming decreased rhizospheric soil inorganic N content in both forests. Our results showed that four-year warming was not beneficial for ECM colonization of P. asperata seedlings in the two forests, and the seedlings in natural forest were more sensitive and flexible to experimental warming than in plantation. The changes of ECM colonization and fine root biomass for effective N uptake would be good for plant growth and remit N leaching under future warming in natural forest
小鼠诱导多能干细胞定向诱导分化为神经嵴样细胞及其体内外移植的研究
No full textThe enteric nervous system (ENS) is termed the "second brain" because of its ability to regulate many important gastrointestinal functions in an autonomous manner. The cells of the ENS originate from the neural crest (NC) during the embryonic development. Neural crest cells (NCCs), which are highly migratory, emigrate from the neural crest, migrate through the mesenchyme to arrive at the upper foregut at E9.5, and finally colonize the whole length of the gut at E14.5. Disturbed migration of NCCs impedes the ENS development, which may lead to many congenital disorders including Hirschsprung's disease (HSCR). HSCR is a common congenital gastrointestinal motility disorder caused by an absence or a reduction of enteric neurons in the distal colon. Cell therapy has been regarded as a potential treatment for this disorder. However, the cell sources for cell therapy have not been fully explored. Previously we established a protocol to induce NCC formation from mouse induced pluripotent stem cells (miPSCs). The protocol includes neurosphere formation, NCC emigration and cell expansion. In the present study, the NCC induction protocol was improved by using an appropriate cell seeding density and adding a small molecule during the emigration stage. An addition of 10 μM of Y27632, an inhibitor of Rho-associated protein kinase (ROCK), to the culture medium during the emigration stage improved the NCC induction efficiency. The percentage of p75 (a NCC marker) positive cells among the emigrated cells was increased to 87.6%. Specific immunostaining results indicated that induced NC-like cells (NCLCs) exhibited capacity to differentiate into neuronal and muscular cells in vitro under specific differentiation conditions. To explore the migration and differentiation abilities of induced NCLCs, an ex vivo hindgut culture system was established. Mouse enteric neural crest cells (ENCCs) were isolated from the whole gut of E13.5-E15.5 embryos to serve as the migration and differentiation control in the ex vivo study. ENCCs and induced NCLCs were transplanted to E12.5 hindgut explants by a microsurgical method, and then the explants were cultured for five days ex vivo. Immunostaining with whole-mount preparations of the explants showed that induced NCLCs migrated from the transplantation site, engrafted to the gut tissues and differentiated into neuronal cells. In addition, after transplanted to the distal colon of mice in vivo, induced NCLCs were found in the muscle layers of the distal colon 14 and 21 days after the transplantation. Although the ability of the transplanted NCLCs to differentiate to different neuronal subtypes in the colon in vivo requires further investigations, our study has established a miPSC-based platform for studies of mouse NCC induction and gave insights into the feasibility of stem cell-based therapy for HSCR.腸道神經系統可以自發調節機體的多種腸道功能，因此也被稱為"第二大腦"。腸道神經系統起源於胚胎期的神經嵴。在小鼠胚胎髮育早期，神經嵴細胞從神經嵴中遷徙出，並按照頭尾方向遷移。在胚胎期9.5天（E9.5）到達前腸上端，隨後在E14.5左右到達後腸末端。神經嵴細胞的遷移過程受損會影響腸道神經系統的早期發育，最終導致一些先天性疾病的發生，比如巨結腸症。巨結腸症是一種常見的先天性腸道蠕動障礙疾病，主要由腸道神經元及/或其前體神經嵴細胞的缺失或減少而導致。近年來，細胞治療被認為是一種有前景的治療方法，但具有治療用途的細胞來源卻沒有得到充分的研究。此前，我們建立了一個體外誘導方法，可以將小鼠誘導多能幹細胞定向誘導分化成為神經嵴樣細胞，其步驟包括神經球的形成，神經嵴細胞的遷移和細胞體外擴增。在本研究中，通過探索合適的成球細胞數目及提高遷移階段的遷移細胞數量來進一步改良此誘導方法。在遷移階段的培養基中加入10μM的Rho相關蛋白激酶（ROCK）抑製劑Y27632可以提高神經嵴樣細胞的誘導效率。在遷移的細胞中，神經嵴細胞標記物p75的陽性率達到87.6%。免疫熒光染色結果表明，在特定的體外培養環境中，誘導的神經嵴樣細胞具有分化成為神經細胞和肌細胞的能力。此外，我們建立了一個後腸體外培養系統。從E13.5-E15.5的EDNRB小鼠全腸中提取腸神經嵴細胞，將其作為離體移植後遷移和分化能力的對照組。隨後通過顯微手術方法將腸神經嵴細胞和誘導神經嵴樣細胞移植至E12.5的後腸中，並在培養基中離體培養5天。全組織免疫熒光染色顯示，誘導神經嵴樣細胞在移植14天后由移植位置遷移進入腸內，並且分化成為神經細胞。此外，在小鼠在體移植實驗中，誘導神經嵴樣細胞被移植至小鼠結腸末端。免疫熒光染色表明，在移植後第14天和第21天神經嵴樣細胞遷移至末端結腸的肌肉層。儘管移植的神經嵴樣細胞在體內的多能分化潛力還需要進一步的探索，但目前我們研究建立了一個基於小鼠誘導多能幹細胞來研究小鼠神經嵴細胞誘導的平台，為巨結腸症的幹細胞療法提供了新的視角和參考。LI, Dandan.Ph.D. Chinese University of Hong Kong 2021.Includes bibliographical references (leaves )Abstracts in English and Chinese.Title from PDF title page (viewed on ...
Application of the BP network evaluation method in desulfurization technology selection of thermo-electricity project
No full textFuzzy comprehensive evaluation on slag-removal system plan selection in thermo-electricity project
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