22 research outputs found
AnoPrimer: Primer Design in malaria vectors informed by range-wide genomic variation.
No full textThe major malaria mosquitoes, Anopheles gambiae s.l and Anopheles funestus, are some of the most studied organisms in medical research and also some of the most genetically diverse. When designing polymerase chain reaction (PCR) or hybridisation-based molecular assays, reliable primer and probe design is crucial. However, single nucleotide polymorphisms (SNPs) in primer binding sites can prevent primer binding, leading to null alleles, or bind suboptimally, leading to preferential amplification of specific alleles. Given the extreme genetic diversity of Anopheles mosquitoes, researchers need to consider this genetic variation when designing primers and probes to avoid amplification problems. In this note, we present a Python package, AnoPrimer, which exploits the Ag1000G and Af1000 datasets and allows users to rapidly design primers in An. gambiae or An. funestus, whilst summarising genetic variation in the primer binding sites and visualising the position of primer pairs. AnoPrimer allows the design of both genomic DNA and cDNA primers and hybridisation probes. By coupling this Python package with Google Colaboratory, AnoPrimer is an open and accessible platform for primer and probe design, hosted in the cloud for free. AnoPrimer is available here https://github.com/sanjaynagi/AnoPrimer and we hope it will be a useful resource for the community to design probe and primer sets that can be reliably deployed across the An. gambiae and funestus species ranges.</p
Erratum to “The SCARE guidelines: Consensus-based surgical case report guidelines” [Int. J. Surg. 34 (2016) 180–186]
No full textThe publisher regrets that there was an error in the author list of this paper. The members of the SCARE Group were not tagged as a collaborator group. This has now been corrected. The names of the collaborator group are as follows: Raafat Afifi, Cairo University Raha Al-Ahmadi, King Faisal Specialist Hospital and Research Centre Joerg Albrecht, John H. Stroger Jr. Hospital of Cook County Abdulrahman Alsawadi, Colchester Hospital University NHS Foundation Trust Jeffrey Aronson, Radcliffe Infirmary, Oxford M. Hammad Ather, Aga Khan University Mohammad Bashashati, Texas Tech University Health Sciences Center Somprakas Basu, Banarus Hindu University Patrick Bradley, Nottingham University Hospitals Mushtaq Chalkoo, Hyderpora Ben Challacombe, Guy's and St Thomas’ NHS Foundation Trust Trent Cross, James Cook University Laura Derbyshire, North West Deanery Naheed Farooq, Central Manchester University Hospital Foundation Trust Jerome Hoffman, University of California Los Angeles Huseyin Kadioglu, Bezmialem Vakif University Veeru Kasivisvanathan, University College London Boris Kirshtein, Soroka University Medical Center Roberto Klappenbach, Simplemente Evita Hospital Daniel Laskin, Virginia Commonwealth University Diana Miguel, University Hospital Jena James Milburn, Queens Medical Centre Seyed Reza Mousavi, Shohada Medical Center Tajrish Oliver Muensterer, University Medicine Mainz James Ngu, Changi General Hospital Iain Nixon, East Kent University Hospitals Ashraf Noureldin, Cumberland Royal Infirmary Benjamin Perakath, Dr. Gray's Hospital Nicholas Raison, King's College London Kandiah Raveendran, Fatimah Hospital Timothy Sullivan, Minneapolis Heart Institute Achilleas Thoma, McMaster University Mangesh Thorat, Wolfson Institue of Preventative Medicine, Queen Mary University of London Michele Valmasoni, Università di Padova Samuele Massarut, Centro di Riferimento Oncologico Aviano, Italy Anil D'cruz, Tata Memorial Hospital Baskaran Vasudevan, MIOT Hospitals Salvatore Giordano, Turku University Hospital Gaurav Roy, Medanta-The Medicity Donagh Healy, University Hospital Waterford David Machado-Aranda, University of Michigan Bryan Carroll, Eastern Virginia Medical School David Rosin, University of West IndiesThe publisher would like to apologise for any inconvenience caused.</p
“The SCARE guidelines: Consensus-based surgical case report guidelines” [Int. J. Surg. 34 (2016) 180–186]((2016) 34 (180–186)(S174391911630303X)(10.1016/j.ijsu.2016.08.014))
No full text© 2016 IJS Publishing Group Ltd The publisher regrets that there was an error in the author list of this paper. The members of the SCARE Group were not tagged as a collaborator group. This has now been corrected. The names of the collaborator group are as follows: Raafat Afifi, Cairo University Raha Al-Ahmadi, King Faisal Specialist Hospital and Research Centre Joerg Albrecht, John H. Stroger Jr. Hospital of Cook County Abdulrahman Alsawadi, Colchester Hospital University NHS Foundation Trust Jeffrey Aronson, Radcliffe Infirmary, Oxford M. Hammad Ather, Aga Khan University Mohammad Bashashati, Texas Tech University Health Sciences Center Somprakas Basu, Banarus Hindu University Patrick Bradley, Nottingham University Hospitals Mushtaq Chalkoo, Hyderpora Ben Challacombe, Guy's and St Thomas’ NHS Foundation Trust Trent Cross, James Cook University Laura Derbyshire, North West Deanery Naheed Farooq, Central Manchester University Hospital Foundation Trust Jerome Hoffman, University of California Los Angeles Huseyin Kadioglu, Bezmialem Vakif University Veeru Kasivisvanathan, University College London Boris Kirshtein, Soroka University Medical Center Roberto Klappenbach, Simplemente Evita Hospital Daniel Laskin, Virginia Commonwealth University Diana Miguel, University Hospital Jena James Milburn, Queens Medical Centre Seyed Reza Mousavi, Shohada Medical Center Tajrish Oliver Muensterer, University Medicine Mainz James Ngu, Changi General Hospital Iain Nixon, East Kent University Hospitals Ashraf Noureldin, Cumberland Royal Infirmary Benjamin Perakath, Dr. Gray's Hospital Nicholas Raison, King's College London Kandiah Raveendran, Fatimah Hospital Timothy Sullivan, Minneapolis Heart Institute Achilleas Thoma, McMaster University Mangesh Thorat, Wolfson Institue of Preventative Medicine, Queen Mary University of London Michele Valmasoni, Università di Padova Samuele Massarut, Centro di Riferimento Oncologico Aviano, Italy Anil D'cruz, Tata Memorial Hospital Baskaran Vasudevan, MIOT Hospitals Salvatore Giordano, Turku University Hospital Gaurav Roy, Medanta-The Medicity Donagh Healy, University Hospital Waterford David Machado-Aranda, University of Michigan Bryan Carroll, Eastern Virginia Medical School David Rosin, University of West IndiesThe publisher would like to apologise for any inconvenience caused
“The SCARE guidelines: Consensus-based surgical case report guidelines” [Int. J. Surg. 34 (2016) 180–186]((2016) 34 (180–186)(S174391911630303X)(10.1016/j.ijsu.2016.08.014))
No full textThe publisher regrets that there was an error in the author list of this paper. The members of the SCARE Group were not tagged as a collaborator group. This has now been corrected. The names of the collaborator group are as follows: Raafat Afifi, Cairo University Raha Al-Ahmadi, King Faisal Specialist Hospital and Research Centre Joerg Albrecht, John H. Stroger Jr. Hospital of Cook County Abdulrahman Alsawadi, Colchester Hospital University NHS Foundation Trust Jeffrey Aronson, Radcliffe Infirmary, Oxford M. Hammad Ather, Aga Khan University Mohammad Bashashati, Texas Tech University Health Sciences Center Somprakas Basu, Banarus Hindu University Patrick Bradley, Nottingham University Hospitals Mushtaq Chalkoo, Hyderpora Ben Challacombe, Guy's and St Thomas’ NHS Foundation Trust Trent Cross, James Cook University Laura Derbyshire, North West Deanery Naheed Farooq, Central Manchester University Hospital Foundation Trust Jerome Hoffman, University of California Los Angeles Huseyin Kadioglu, Bezmialem Vakif University Veeru Kasivisvanathan, University College London Boris Kirshtein, Soroka University Medical Center Roberto Klappenbach, Simplemente Evita Hospital Daniel Laskin, Virginia Commonwealth University Diana Miguel, University Hospital Jena James Milburn, Queens Medical Centre Seyed Reza Mousavi, Shohada Medical Center Tajrish Oliver Muensterer, University Medicine Mainz James Ngu, Changi General Hospital Iain Nixon, East Kent University Hospitals Ashraf Noureldin, Cumberland Royal Infirmary Benjamin Perakath, Dr. Gray's Hospital Nicholas Raison, King's College London Kandiah Raveendran, Fatimah Hospital Timothy Sullivan, Minneapolis Heart Institute Achilleas Thoma, McMaster University Mangesh Thorat, Wolfson Institue of Preventative Medicine, Queen Mary University of London Michele Valmasoni, Università di Padova Samuele Massarut, Centro di Riferimento Oncologico Aviano, Italy Anil D'cruz, Tata Memorial Hospital Baskaran Vasudevan, MIOT Hospitals Salvatore Giordano, Turku University Hospital Gaurav Roy, Medanta-The Medicity Donagh Healy, University Hospital Waterford David Machado-Aranda, University of Michigan Bryan Carroll, Eastern Virginia Medical School David Rosin, University of West IndiesThe publisher would like to apologise for any inconvenience caused
A probability‐based model to quantify the impact of hydropeaking on habitat suitability in rivers
No full textAbstractA negative effect of hydropower on river environment includes rapid changes in flow and habitat conditions. Any sudden flow change could force fish to move towards a refuge area in a short period of time, causing serious disturbances in the life cycle of the fish. A probability-based model was developed to quantify the impact of hydropeaking on habitat suitability for two fish species, brown trout (Salamo trutta) and Grayling (Thymallus thymallus). The model used habitat preference curves, river velocity and depth to develop the suitability maps. The suitability maps reveal that habitat suitability deteriorates as flow increases in the studied part of the river. The probability model showed that, on average, suitability indices are higher for adult grayling than juvenile trout in hydropeaking events in this part of the river. The method developed shows the potential to be used in river management and the evaluation of hydropeaking impacts in river systems affected by hydropower.Abstract
A negative effect of hydropower on river environment includes rapid changes in flow and habitat conditions. Any sudden flow change could force fish to move towards a refuge area in a short period of time, causing serious disturbances in the life cycle of the fish. A probability-based model was developed to quantify the impact of hydropeaking on habitat suitability for two fish species, brown trout (Salamo trutta) and Grayling (Thymallus thymallus). The model used habitat preference curves, river velocity and depth to develop the suitability maps. The suitability maps reveal that habitat suitability deteriorates as flow increases in the studied part of the river. The probability model showed that, on average, suitability indices are higher for adult grayling than juvenile trout in hydropeaking events in this part of the river. The method developed shows the potential to be used in river management and the evaluation of hydropeaking impacts in river systems affected by hydropower
Isolation of Gram-positive Bacteria from Different Sources and Evaluation of their Probiotic Properties
No full textFixed structured H<inf>∞</inf> controller design for aircraft control surface driven by power by wire hydraulic actuator
No full textConservation in an Islamic context a case study of Makkah
No full textThe Holy Qu’ rān contains many injunctions for Muslims to respect and conserve the natural environment but few address the built environment. Habitat at the time of the Prophet (PBOH) was in the vernacular and relatively impermanent. The first habitat was the cave, the second the tent and then simple flat roofed buildings of post and lintel construction made of mud and rubble. Later buildings were not indigenous but reflected the architectural styles and techniques of Muslim pilgrims from beyond the Arabian Peninsula. Permanent exotic buildings were later erected as reminders of holy places and events. This work advances a case to restore and preserve historic and religious sites in Makkah, Saudi Arabia. Makkah is the destination for millions of Muslim pilgrims who annually pay homage to Allah during the occasions of Hajj, Ramadan and Umra. The tranquillity and peaceful ambience that one associates with the holiest of Islamic experiences have, over the years, given way to jostling crowds of people who must be expediently housed, fed, transported, and protected. Due to the lack of planning and the insensitive but profitable development of the city, Makkah is in grave danger of becoming a bustling metropolis instead of a sanctuary where pilgrims gather to perform their religious rites and reaffirm their dedication to Allah. The author calls for professional planning and international cooperation to guide future development for this expanding and sensitive area. The author's ideas are grounded in practical and aesthetic study, therefore, the political, environmental and economic issues are examined in relationship to religious, historic and artistic values. The author makes proposals for a future Makkah that would provide pilgrims with the physical comforts, security, and serene environment they deserve—without destroying the city they came to visit. The author discusses preservation and conservation in the western world and the need for their acceptance in Muslim countries, the former being an aesthetic and intellectual concept sustained by law and the latter being the prescribed free expression of the individual unhindered by material considerations. Both worlds are rapidly being overwhelmed by materialism, but body, mind and spirit combine in making us aware of our surroundings and the way in what we see around us has come into being
Assessment of Perimenopausal Bleeding Using Three-Dimensional Hysterography, Hysteroscopy, and Histopathology
No full textA reappraisal of attitudes to the 'People of the Book' in the Qur'an and hadith, with particular reference to Muslim fiscal policy and the covenant of 'Umar
No full textEThOS - Electronic Theses Online ServiceGBUnited Kingdo
