2 research outputs found
Support STL files for a Spectroelectrochemical 3D designed cell for magnetic nanostirring
No full textSTL Files for the 3D Printing of a Customized Spectroelectrochemical Cell for Magnetic Nanostirring Applications on the SPELEC Instrument (Metrohm)
This dataset contains STL files for the 3D printing of a customized spectroelectrochemical (SEC) cell specifically designed to enable magnetic nanostirring during SEC measurements using the SPELEC instrument (Metrohm). The original design was created using FreeCAD software.
The printed cell used in experimental studies was fabricated in black polylactic acid (PLA) using an XYZPrinter da Vinci 1.0 Pro equipped with a 0.4 mm nozzle. The design includes two modular parts forming the main cell body, incorporating a 4-pillar structure to ensure proper sealing and minimize external light interference during measurements. This configuration allows for the use of magnetic nanostirring with sample volumes as low as 50 µL when placed on a standard magnetic stirrer platform.
The closure mechanism in this design provides an alternative to the standard commercial SEC cell, which uses a magnetic coupling closure system that is not compatible with magnetic nanostirring under equivalent experimental conditions.
The files are provided in STL format and can be directly used for additive manufacturing using common FDM 3D printers
Hydrolysis of Lactose: Conventional Techniques and Enzyme Immobilization Strategies on Polymeric Supports
No full textThis chapter explores lactose hydrolysis, emphasizing conventional techniques
and the noteworthy immobilization of β-galactosidase on polymeric matrices to
enhance the process. Lactose, present in milk and dairy, poses challenges for lactoseintolerant
individuals, requiring enzymatic hydrolysis for lactose-free product development.
The presence of other milk components, such as proteins and minerals, can
indirectly influence the efficiency of lactose hydrolysis by potentially interacting with
β-galactosidase enzyme or affecting its stability and activity, making it necessary to
control factors such as enzyme concentration, temperature, pH, and reaction time to
improve lactose hydrolysis rates. The chapter delves into established methodologies,
covering enzymatic kinetics, reaction conditions, and substrate concentrations. It
also describes the innovative approach of immobilizing β-galactosidase on polymeric
supports to enhance enzyme stability, reusability, and overall efficiency in lactose
hydrolysis. Discussions include the design of suitable polymeric matrices, providing
insights into mechanisms governing catalytic performance. This comprehensive
exploration contributes to understanding lactose hydrolysis, offering valuable insights
for developing efficient and sustainable enzymatic processes applicable to the food
and pharmaceutical industries.We gratefully acknowledge the financial support provided by all funders. This work was supported by the Regional Government of Castilla y León (Junta de Castilla y León) and by the Ministry of Science and Innovation MICIN and the European Union NextGenerationEU PRTR. Author Saul Vallejos received grant BG22/00086 funded by the Spanish Ministerio de Universidades. Author Jose Miguel García received grant PID2020-113264RB-I00 funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe,” The financial support provided by Fondo Europeo de Desarrollo Regional-European Regional Development Fund (FEDER, ERDF) and Regional Government of Castilla y León -Consejería de Educación, Junta de Castilla y León- (BU025P23) is gratefully acknowledged
