6,146 research outputs found
Cation permeability and cation-anion interactions in a mutant GABA-gated chloride channel from Drosophila
No full textDoes resistance really carry a fitness cost?
No full textThis is the final version of the article. Available from Elsevier via the DOI in this record.Insecticide resistance mutations are widely assumed to carry fitness costs. However studies to measure such costs are rarely performed on genetically related strains and are often only done in the laboratory. Theory also suggests that once evolved the cost of resistance can be offset by the evolution of fitness modifiers. But for insecticide resistance only one such example is well documented. Here we critically examine the literature on fitness costs in the absence of pesticide and ask if our knowledge of molecular biology has helped us predict the costs associated with different resistance mechanisms. We find that resistance alleles can arise from pre-existing polymorphisms and resistance associated variation can also be maintained by sexual antagonism. We describe novel mechanisms whereby both resistant and susceptible alleles can be maintained in permanent heterozygosis and discuss the likely consequences for fitness both in the presence and absence of pesticide. Taken together these findings suggest that we cannot assume that resistance always appears de novo and that our assumptions about the associated fitness costs need to be informed by a deeper understanding of the underlying molecular biology.Work on insecticide resistance in the ffrench-Constant and Bass laboratories is supported by the BBSRC (BB/H014268 to R. ff-C), the Royal Society (Wolfson Merit Award to R. ff-C) and the ERC (ERC Consolidator award to CB)
Butterfly gene flow goes berserk
No full textThis is the final version of the article. Available from the publisher via the DOI in this record.A new study shows that genomic introgression between two Heliconius butterfly species is not solely confined to color pattern loci.The authors work on butterfly wing patterning is supported by the BBSR
Transposable elements and xenobiotic resistance
No full textTransposable elements or TEs are well known drivers of adaptive change in plants and animals but their role in insecticide resistance remains poorly documented. This review examines the potential role of transposons in resistance and identifies key areas where our understanding remains unclear. Despite well-known model systems such as upregulation of Drosophila Cyp6g1, many putative examples lack functional validation. The potential types of transposon-associated changes that could lead to resistance are reviewed, including changes in up-regulation, message stability, loss of function and alternative splicing. Where potential mechanisms appear absent from the resistance literature examples are drawn from other areas of biology. Finally, ways are suggested in which transgenic expression could be used to validate the biological significance of TE insertion. In the absence of such functional expression studies many examples of the association of TEs and resistance genes therefore remain as correlations
Butterfly gene flow goes berserk
No full textA new study shows that genomic introgression between two Heliconius butterfly species is not solely confined to color pattern loci
Actions of Agonists, Fipronil and Ivermectin on the Predominant In Vivo Splice and Edit Variant (RDLbd, I/V) of the Drosophila GABA Receptor Expressed in Xenopus laevis Oocytes
No full textIonotropic GABA receptors are the targets for several classes of insecticides. One of the most widely-studied insect GABA receptors is RDL (resistance to dieldrin), originally isolated from Drosophila melanogaster. RDL undergoes alternative splicing and RNA editing, which influence the potency of GABA. Most work has focussed on minority isoforms. Here, we report the first characterisation of the predominant native splice variant and RNA edit, combining functional characterisation with molecular modelling of the agonist-binding region. The relative order of agonist potency is GABA> muscimol> TACA> β-alanine. The I/V edit does not alter the potency of GABA compared to RDLbd. Docking calculations suggest that these agonists bind and activate RDLbdI/V through a similar binding mode. TACA and β-alanine are predicted to bind with lower affinity than GABA, potentially explaining their lower potency, whereas the lower potency of muscimol and isoguvacine cannot be explained structurally from the docking calculations. The A301S (resistance to dieldrin) mutation reduced the potency of antagonists picrotoxin, fipronil and pyrafluprole but the I/V edit had no measurable effect. Ivermectin suppressed responses to GABA of RDLbdI/V, RDLbd and RDLbdI/VA301S. The dieldrin resistant variant also showed reduced sensitivity to Ivermectin. This study of a highly abundant insect GABA receptor isoform will help the design of new insecticides
Dimensional instability of cement bonded particleboard - Part 2: Behaviour and its prediction under cyclic changes in RH
No full textThis is a post print version of the article. The official published version can be obtained from the link below.This paper examines the dimensional instability of cement bonded particleboard (CBPB) and discusses the behaviour of CBPB during cyclic changes in relative humidities (RH). The results indicated that the changes of CBPB in both mass and dimensions reflected the changes in RH; in a cycle 90–65–35–65–90%RH the change of per percentage change in RH was 1.0:1.3:1.3:2.5 for mass, 1:2:2:1 for length and 1.3:1.1:1.0:1.0 for thickness. The changes were closely related to the structure of CBPB and the nature of the cement paste and wood chips. Thickness change was double that of the length change between 65 and 35%RH and triple that between 65 and 90%RH. The rate of change in mass was much higher than that in dimensions. The hysteresis loops were dissimilar to those of other wood and wood-based materials, with the loops of mass change closed between 65 and 90%RH, while the loops for dimensional changes were open. A set of the former loops moved upward and the latter moved downward with increasing number of cycles, corresponding to an accumulated increase in mass, but decrease in dimensions. The models developed for CBPB under constant and a single changing RH were successfully applied to changes under cyclic RH.
The sorption behaviour and dimensional movement of CBPB has been shown to be influenced not only by RH, but also by its intermediate history, with the maximum values for change within every phase of sorption, in both mass and dimensions, being higher for the cycling between 35–90%RH than the cycling regime 35–65–90%RH. The ratios of dimensional changes to mass change of CBPB were greater under the latter regime than under the former regime.Partly Financial Support from the British Council
New results from previously reported NBS fundamental constant determinations
No full textA new treatment of previously reported results of three electric-unit-dependent fundamental constant experiments carried out at NBS over the last decade or so yields accurate, indirect values in SI units for a number of important quantities. These include the fine-structure constant alpha, the Avogadro constant NA, the Josephson frequency-voltage ratio 2e/h, and the quantized Hall resistance RH=h/e2
Does resistance really carry a fitness cost?
No full textInsecticide resistance mutations are widely assumed to carry fitness costs. However studies to measure such costs are rarely performed on genetically related strains and are often only done in the laboratory. Theory also suggests that once evolved the cost of resistance can be offset by the evolution of fitness modifiers. But for insecticide resistance only one such example is well documented. Here we critically examine the literature on fitness costs in the absence of pesticide and ask if our knowledge of molecular biology has helped us predict the costs associated with different resistance mechanisms. We find that resistance alleles can arise from pre-existing polymorphisms and resistance associated variation can also be maintained by sexual antagonism. We describe novel mechanisms whereby both resistant and susceptible alleles can be maintained in permanent heterozygosis and discuss the likely consequences for fitness both in the presence and absence of pesticide. Taken together these findings suggest that we cannot assume that resistance always appears de novo and that our assumptions about the associated fitness costs need to be informed by a deeper understanding of the underlying molecular biology
Drosophila embryos as model systems for monitoring bacterial infection in real time
No full textDrosophila embryos are well studied developmental microcosms that have been used extensively as models for early development and more recently wound repair. Here we extend this work by looking at embryos as model systems for following bacterial infection in real time. We examine the behaviour of injected pathogenic (Photorhabdus asymbiotica) and non-pathogenic (Escherichia coli) bacteria and their interaction with embryonic hemocytes using time-lapse confocal microscopy. We find that embryonic hemocytes both recognise and phagocytose injected wild type, non-pathogenic E. coli in a Dscam independent manner, proving that embryonic hemocytes are phagocytically competent. In contrast, injection of bacterial cells of the insect pathogen Photorhabdus leads to a rapid ‘freezing’ phenotype of the hemocytes associated with significant rearrangement of the actin cytoskeleton. This freezing phenotype can be phenocopied by either injection of the purified insecticidal toxin Makes Caterpillars Floppy 1 (Mcf1) or by recombinant E. coli expressing the mcf1 gene. Mcf1 mediated hemocyte freezing is shibire dependent, suggesting that endocytosis is required for Mcf1 toxicity and can be modulated by dominant negative or constitutively active Rac expression, suggesting early and unexpected effects of Mcf1 on the actin cytoskeleton. Together these data show how Drosophila embryos can be used to track bacterial infection in real time and how mutant analysis can be used to genetically dissect the effects of specific bacterial virulence factors
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