268 research outputs found
Oppioidi ed attivazione puberale: regolazione della funzione surrenalica e riproduttiva e della secrezione gonadotropinica. Ginecologia dell'Infanzia e dell'adolescenza
No full text.
SIMULTANEOUS RIA OF HUMAN BETA-ENDORPHIN AND BETA-LIPOTROPIN IN PLASMA EXTRACTS
No full textThe identification of f)-endorphin (f)-EP) as part of the larger precurs~_
f)-lipotropin (f)-LPH) in pituitary cells and the importance of f)-E P in
behaviour and endogenous analgesia dictate the need for a method of measur-
ing the plasma or li.quour concentrations of them in physiological and path-
ological conditions. The present study concerns the deve:.opment of RIA
methods to measure f)-EP ~:Id f)-LPH after extraction and pu r i f i c a t i.o n . Synt:"
etic f)-EP (Peninsular Inc., San Francisco) and h f)-LPH (Dr.C.H.Li, San Fra~
cisco) and h f)-EP antiserum (Dr.C.Pert NIH, Bethesda) were used.
8nth peptides were labelled with Il~5 using the chloramine T method, an
then purified by gel filtration in a Sephadex G-25 culumn. The specific
activity of f)-EP-I125 was lSOuC/ug and that of f)-LPH-I125 was 242uC/ug.
The specificity of the antiserum using both labelled peptides was chec~
with the following substances: leu-enkephalin, Ret-enkephalin, substance P
GIF, 1-39 ACTH, f)-MSH, 61-77 f)-LPH, 79-91 f)-LPH, f)-E P and f)-LPH. No cross
reaction was found with leu and met-enkephalin, substance P, GIF and 1-39
ACTH, while in the f)-EP and f)-LPH RIAs, f)-LPH and f)-EP showe d a cross r e ac t-
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