1,763,566 research outputs found
DEP-1 coordinates β-integrin activation with EGFR signaling.
No full textLeft side: DEP-1 controls integrin activation by de-phosphorylating the membrane proximal NPXY792 residue in the β-integrin PAT-3. This promotes the recruitment of TLN-1 talin to focal adhesion sites, which attenuates EGFR trafficking and activity. Right side: DEP-1 directly de-phosphorylates the EGFR to inhibit RAS/MAPK activation.</p
DEP: Beyond epenthesis
No full textDEP is often informally characterized as a constraint against epenthesis, but its actual effects are more diverse. In the domain of base-reduplicant correspondence, DEP can have the effect of blocking deletion in the base, as in Tonkawa reduplication. In the domain of output-output correspondence, DEP in effect requires deletion of underlying material in derived forms, as in English. These consequences follow straightforwardly from the correspondence-theoretic definition of DEP as a bi-level faithfulness constraint, and they cannot be derived without such a constraint.The publisher of the journal in which this article appears does not permit the archiving of this or any other version of the article in the Rutgers Optimality Archive. The authorized version is available here: http://www.jstor.org/stable/4007141
DEP-1 de-phosphorylates the NPXY<sub>792</sub> motif in PAT-3.
No full text(A) Binding of PAT-3::GFP and PAT-2::GFP to DEP-1intra-DA. Total proteins extracts from the pat-3::gfp strain qIs43 (top panel) and the pat-2::gfp strain jeIs2222 (bottom panel) were incubated with the indicated GST fusion proteins and bound proteins were detected on anti-GFP Western blots. The Coomassie blue stained gels showing the purified GST fusion proteins used for this assay are shown in S1 Fig. (B) The interaction of DEP-1intra-DA with PAT-3::GFP is inhibited by the addition of 5 mM Na3VO4. (C) Sequence alignment of the C. elegans PAT-3 and human β2, β7, β1, β3, and β6 integrin cytoplasmic tails. Conserved tyrosine residues are highlighted in blue and NPXY domains are marked in grey. (D) Interaction of DEP-1intra-DA and DEP-1intra-wt with different PAT-3::GFP mutants containing Tyr to Ala or Thr to Ala exchanges. (E) Quantification of the binding experiments shown in (D). For each mutant construct, the average binding measured in three independent experiments is shown. Intensities of the bands were determined by densitometry, normalized to the input bands (bottom panel) and the values are shown as % of the binding detected with wild-type PAT-3::GFP. p-values are indicated with *** p<0.001 and ** p<0.01 in a two tailed student’s t-test—two-sample unequal variance. Error bars show the standard error of the mean.</p
Shortening the Edinburgh postnatal depression scale using optimal test assembly methods: Development of the EPDS-Dep-5
No full textAbstractAimsThis study used a large database to develop a reliable and valid shortened form of the Edinburgh Postnatal Depression Scale (EPDS), a self‐report questionnaire used for depression screening in pregnancy and postpartum, based on objective criteria.MethodsItem responses from the 10‐item EPDS were obtained from 5157 participants (765 major depression cases) from 22 primary screening accuracy studies that compared the EPDS to the Structured Clinical Interview for DSM (SCID). Unidimensionality of the EPDS latent construct was verified using confirmatory factor analysis, and an item response theory model was fit. Optimal test assembly (OTA) methods identified a maximally informative shortened form for each possible scale length between 1 and 9 items. The final shortened form was selected based on pre‐specified validity and reliability criteria and non‐inferiority of screening accuracy of the EPDS as compared to the SCID.ResultsA 5‐item short form of the EPDS (EPDS‐Dep‐5) was selected. The EPDS‐Dep‐5 had a Cronbach's alpha of 0.82. Sensitivity and specificity of the EPDS‐Dep‐5 for a cutoff of 4 or greater were 0.83 (95% CI, 0.73, 0.89) and 0.86 (95% CI, 0.80, 0.90) and were statistically non‐inferior to the EPDS. The correlation of total scores with the full EPDS was high (r = 0.91).ConclusionThe EPDS‐Dep‐5 is a valid short form with minimal loss of information when compared to the full‐length EPDS. The EPDS‐Dep‐5 was developed with OTA methods using objective, pre‐specified criteria, but the approach is data‐driven and exploratory. Thus, there is a need to replicate results of this study in different populations
The Kimberley assessment of depression of older Indigenous Australians: prevalence of depressive disorders, risk factors and validation of the KICA-dep scale
Get PDFThis study aimed to develop a culturally acceptable and valid scale to assess depressive symptoms in older Indigenous Australians, to determine the prevalence of depressive disorders in the older Kimberley community, and to investigate the sociodemographic, lifestyle and clinical factors associated with depression in this population.
Methods
Cross-sectional survey of adults aged 45 years or over from six remote Indigenous communities in the Kimberley and 30% of those living in Derby, Western Australia. The 11 linguistic and culturally sensitive items of the Kimberley Indigenous Cognitive Assessment of Depression (KICA-dep) scale were derived from the signs and symptoms required to establish the diagnosis of a depressive episode according to the DSM-IV-TR and ICD-10 criteria, and their frequency was rated on a 4-point scale ranging from ‘never’ to ‘all the time’ (range of scores: 0 to 33). The diagnosis of depressive disorder was established after a face-to-face assessment with a consultant psychiatrist. Other measures included sociodemographic and lifestyle factors, and clinical history.
Results
The study included 250 participants aged 46 to 89 years (mean±SD = 60.9±10.7), of whom 143 (57.2%) were women. The internal reliability of the KICA-dep was 0.88 and the cut-point 7/8 (non-case/case) was associated with 78% sensitivity and 82% specificity for the diagnosis of a depressive disorder. The point-prevalence of a depressive disorder in this population was 7.7%; 4.0% for men and 10.4% for women. Heart problems were associated with increased odds of depression (odds ratio = 3.3, 95% confidence interval = 1.2,8.8).
Conclusions
The KICA-dep has robust psychometric properties and can be used with confidence as a screening tool for depression among older Indigenous Australians. Depressive disorders are common in this population, possibly because of increased stressors and health morbidities
DEP-induced IL-6 and IL-8 expression and its suppression by signaling pathway inhibitors in <i>ex vivo</i> organ culture.
No full textNasal inferior turbinate tissues were cultured ex vivo and treated with DEP (50 μg/mL). Expression of IL-6 and IL-8 was increased after treatment, as determined by ELISA. The increased expression was blocked by pretreatment with SB203580 (10 μmol/L), LY294002 (10 μmol/L), or BAY117082 (1uM). The graphic data represents the means ± SEM of three independent experiments. * p<0.05 compared to control; † p<0.05, †† p<0.01 compared to treatment with DEP alone.</p
External validation of a shortened screening tool using individual participant data meta-analysis: A case study of the Patient Health Questionnaire-Dep-4
No full textExternal validation of a shortened screening tool using individual participant data meta-analysis: A case study of the Patient Health Questionnaire-Dep-
DEP-1 promotes the recruitment of TLN-1 to the basolateral plasma membrane.
No full text(A) Expression of the endogenous wild-type PAT-3::GFP reporter zh115 in a wild-type and (B) a dep-1(lf); lip-1(lf) late L3 larva at the onset of vulval invagination. (C) Expression of the endogenous Y792F mutant PAT-3::GFP reporter zh116 in a wild-type L3 larva. The insets in (A), (B) and (C) show the 2° VulD cells with the dashed yellow boxes indicating 5 μm long and 1.5 μm wide regions used to quantify PAT-3::GFP distribution. The asterisks indicate the position of the AC. (D) Average intensity profiles of the PAT-3::GFP signal in the regions shown in the insets of (A), (B) and (C). For each animal, the intensities were normalized to the summed intensities and the individual profiles were combined into the average profiles shown in (D), as described [35]. (E) Quantification of the membrane recruitment of PAT-3::GFP. The standard deviations of the mean intensities in the graphs shown in (D) were taken as a measure for uneven PAT-3::GFP distribution, indicating membrane recruitment [35]. (F) Expression of the endogenous GFP::TLN-1 reporter zh117 in a wild-type, (G) a dep-1(lf); lip-1(lf) and (H) a pat-3(Y792F) mutant L3 larva. (I) Average intensity profiles of the GFP::TLN-1 signal in the regions shown in the insets of (F), (G) and (H), and (K) quantification of the membrane recruitment of GFP::TLN-1 were done as described above under (D) and (E). The error bars indicate the 95% confidence intervals, and the values in brackets the numbers of animals analyzed for each genotype. p-values in (K) determined with ANOVA followed by Bonferroni correction are indicated with ** p<0.01 and * p<0.05. The scale bar in (H) represents 10 μm.</p
Seminar hukum nasional ke-vii reformasi hukum menuju masyarakat madani/ Dep. Kehakiman
No full textviii, 538 hal.; 21 cm
Himpunan keputusan menteri kehakiman RI tahun 1996 s.d 1998/ Dep. Kehakiman
No full textviii, 1437 hal; 21 cm
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