1,721,053 research outputs found
A low-noise transimpedance amplifier for BLM-Based ion channel recording
No full textData for the paper "Crescentini, Marco, Bennati, Marco, Saha, Shimul C., Ivica, Josip, de Planque, Maurits R.R., Morgan, Hywel and Tartagni, Marco (2016) A low-noise transimpedance amplifier for BLM-based ion channel recording. Sensors, 16, (5, 709), 1-20. (doi:10.3390/s16050709)."</span
Developing microscale apertures for ion channel recordings with ultra-stable lipid bilayer membranes
No full textLipid bilayer platforms for parallel ion channel recordings
No full textThe ion flow through channel proteins embedded in a lipid bilayer membrane can be recorded as an electrical current, enabling biophysical characterization and pharmacological drug screening at a single-channel level. These measurements are challenging because the self-assembled bilayers are fragile and the currents are in the pA–nA range. This concise review introduces the bilayer recording methodology, with an emphasis on the requirements for full electrophysiology assays. The self-assembled lipid bilayer, formed in a ∼100 μm diameter aperture in between two aqueous chambers, is critical. Various approaches to increase the measurement throughput by scaling to aperture arrays are discussed in terms of current-amplifier technology, bilayer stability, ion channel incorporation, system functionality and obtained single-channel data. The various bilayer recording platforms all have advantages and limitations. Combining the strengths of the different platform architectures, for example, the use of shaped apertures, will be essential to realize and also automate parallel ion channel recordings
Salt gradient modulation of microRNA translocation through a biological nanopore
No full textCurrent traces, histograms and graphs (Figs. 1-4): data as Excel files.
Supports the paper Ivica, J., Williamson, P. T. F., & De Planque, M. R. R. (2017). Salt gradient modulation of microRNA translocation through a biological nanopore. Analytical Chemistry, 89(17), 8822-8829. DOI: 10.1021/acs.analchem.7b01246</span
Nanopore resistive pulse sensing with multiple alpha-hemolysin pores improves the detection limit of microRNA
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