1,786,544 research outputs found
SDS-PAGE gel showing the expression levels of PRC1-DD WT, mutant PRC1-DD-N+4 and PRC1-DD-N+His.
No full textEach lane shows the eluted protein profile with induction time labeled on top of each lane. The expression system in E.Coli/Rosseta (DE3). The molecular weight of PRC1-DD is around 15kD. (PDF)</p
DD-Akt(E40K) is inducible in a neuronal context.
No full text(A) Western immunoblot analysis of DD-Akt(E40K) induction by TMP in neuronal PC12 cells. Neuronal PC12 cells were infected as indicated with a lentiviral vector expressing either eGFP alone (pWPI) or the DD-Akt(E40K) fusion gene and eGFP. Four days post-infection, cells were treated with or without 10 μM TMP as indicated for 24 hr and then harvested for western immunoblotting. Blots were probed as indicated for total Akt, pAkt(pT308), pAkt(pS473) and GAPDH (loading control). A representative blot is shown. (B) Average fold induction of DD-Akt forms by TMP treatment in neuronal PC12 cultures. N = 4–6 independent experiments with duplicate or triplicate wells per condition. Graph shows means with SEM. *p ≤ 0.05, paired t-test, +TMP vs.–TMP levels. (C) The relative expressions of induced DD-pAkt(E40K) and endogenous pAkt levels in neuronal PC12 cells quantified and calculated as a percent ratio. (D) Western immunoblot analysis of total and phospho-S473 forms of DD-Akt(E40K) induction by TMP in cultured rat cortical neurons. Cultured rat cortical neurons were infected as indicated with a lentiviral vector expressing either eGFP alone (pWPI) or the DD-Akt(E40K) fusion gene and eGFP. Four days post-infection, cells were treated with or without 10 μM TMP as indicated for 24 hr and then harvested for western immunoblotting. Blots were probed as indicated for total Akt, pAktT308, pAktS473 and GAPDH (loading control). Assessment of GFP expression indicated an infection efficiency of about 50% of the neurons. A representative blot is shown. (E). Average fold induction of total and DD-pAkt(E40K-pS473) by TMP treatment in cortical neuron cultures. N = 2 samples per condition. Values are means with SEM. *p<0.05, **p<0.01, unpaired t-test, +TMP vs -TMP.</p
DD-Akt(E40K) induction is dose-responsive to TMP treatment and reversible.
No full text(A) Western immunoblotting analysis of dose-response of DD-Akt(E40K) induction by TMP. HEK293 cells were transfected with eGFP-only expressing vector (EV) or DD-Akt(E40K) fusion protein expression vector and treated with indicated doses of TMP for 24 hr before analysis by western immunoblotting with the indicated probes. (B) Quantification of DD-Akt(E40K) induction (as determined by western immunoblotting) by various doses of TMP. Values means with SEM. N = 3–4 independent experiments. (C) Time course of DD-Akt(E40K) induction by TMP. HEK293 cells were transfected with DD-Akt(E40K) expression vector, treated with 10 μM TMP for the indicated times and then assessed for total DD-Akt(E40K) levels by western immunoblotting as in (A). Values are means with SEM. N = 2–4 independent experiments. (D) Reversal of DD-Akt(E40K) induction after TMP washout. HEK293 cultures transfected with DD-Akt(E40K) as above were treated with 10 μM TMP for 24 hr, washed free of TMP and harvested for western immunoblotting at the indicated time points. Total DD-Akt(E40K) levels were determined by western immunoblotting as in (A). Data shown are from one experiment.</p
Structure diagram of Improved MIMO-DD-3Q Learning model.
No full textStructure diagram of Improved MIMO-DD-3Q Learning model.</p
Overview of HBV integration sites in TCGA-DD-A1EL.
No full textOverview of HBV integration sites in TCGA-DD-A1EL.</p
D’un anniversaire à l’autre… 10 ans à la tête de DD&T
Get PDFLa revue Développement Durable & Territoires (DD&T) a fêté il y a peu l’anniversaire de ses vingt ans de parution. Un numéro riche qui a permis – une fois n’est pas coutume – de rassembler les membres de la revue autour de rubriques revisitées, pour une mobilisation collective joyeuse et nourrie. À mon tour à présent par le biais de cet édito, qui prend la forme d’un billet personnel, de fêter un anniversaire…… mes dix ans à la tête du Comité de rédaction de DD&T, toujours en collaboration e..
DD-DeCaF/id-mapper: Initial release
No full text<p>This is the initial release for the deliverable D8.1 in the DD-DeCaF proposal.</p>
Diurnal locomotor activity of turbot in LD-DD-LD lighting conditions.
No full textA, D) Representative actograms of turbot swimming activity. Recording activity during 33 days is represented by double plot of two consecutive days. The white and black bars above the actograms indicate light and dark periods. The arrows above the white bar indicate the time where food is delivered. Fish were kept in LD and fed at ZT2 for 33 days (A), or randomly fed during the light phase (D). From day 12 to 14 fish were in DD and then returned to LD. Fishes showed daily periodicity in LD (B, E), but not in DD (C, D). Turbot displayed its activity mainly during the light phase.</p
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