6 research outputs found
Perspective on in vivo SPME for human applications: Starting from monitoring doxorubicin during lung chemo-perfusion
No full text
Preparation and Application of Composite Lightweight Aggregate Concrete Self-Insulation Block
No full textEmbedding Mixed Sorbents in Binder: Solid-Phase Microextraction Coating with Wide Extraction Coverage and Its Application in Environmental Water Analysis
No full textSolid-phase
microextraction (SPME) is a simple and highly effective
sample-preparation technique for water analysis. However, the extraction
coverage of a given SPME device with a specific coating can be an
issue when analyzing multiple environmental contaminants. Therefore,
instead of synthesizing one sorbent material with dual or multiple
functions, we investigated a new strategy of preparing SPME blades
using a homogeneous slurry made by mixing three different sorbent
particlesnamely, hydrophobic/lipophilic balanced (HLB), HLB-weak
cationic exchange (HLB-WCX), and HLB-weak anionic exchange (HLB-WAX)with
a polyacrylonitrile (PAN) binder. The developed coating is matrix
compatible, as the binder functions not only as a glue for immobilizing
the sorbent particles but also as a porous filter, which only allows
small molecules to enter the pores and interact with the particles,
thus avoiding contamination from large elements. The results confirmed
that the proposed mixed-coating SPME device provides good extraction
performance for polar and nonpolar as well as positively and negatively
charged compounds. Based on this device, three comprehensive analytical
methodologieshigh-throughput SPME–LC–MS/MS (for
the quantitative analysis of targeted drugs of abuse and artificial
sweeteners), in-bottle SPME–LC-high resolution MS (HRMS) (for
the untargeted screening of organic contaminants), and on-site drone
sampling SPME–LC-HRMS (for on-site sampling and untargeted
screening)were developed for use in environmental water analysis.
The resultant data confirm that the proposed strategies enable comprehensive
water quality assessment by using a single SPME device
The Significance of Photoelectric Technology in the Study of Ceramics Unearthed from the Zhouqiao Site in Kaifeng, Henan Province
Get PDFThis article firstly introduces recent dilemmas of traditional archaeological methods in archaeological studies. To solve these problems, bringing in modern scientific technologies is necessary. Then explaining working principles of photoelectric technology equipment like TEM, SEM, XRF, XPS, and their applications in field of archaeology. Finally in a case study of unearthed ceramics from Zhouqiao site in Kaifeng, listing some practical academic problems can be solved by above technologies
Proteomic Analysis of Human Saliva via Solid-Phase Microextraction Coupled with Liquid Chromatography–Mass Spectrometry
No full textProteomics of human saliva samples was achieved for the
first time
via biocompatible solid-phase microextraction (bio-SPME) devices.
Upon introduction of a porogen to a conventional C18 coating, porous
C18/polyacrylonitrile (PAN) SPME blades were able to extract peptides
up to 3.0 kDa and more peptides than commercial SPME blades. Following
Trypsin digestion, salivary proteomic analysis was achieved via SPME-LC-MS/MS.
Seven endogenous proteins were consistently identified in all saliva
samples via bio-SPME. Taking advantage of this strategy, untargeted
peptidomics was applied for the comparison of saliva samples between
healthy and SARS-CoV-2 positive individuals. The results showed clear
peptidomic differences between the viral and healthy saliva samples.
This proof-of-concept study demonstrates the potential of bio-SPME-LC-MS/MS
for peptidomics and proteomics in biomedical applications
