560 research outputs found
Acid hydrolysis of corn stover for biohydrogen production using Thermoanaerobacterium thermosaccharolyticum W16
No full textPiCO QL: A software library for runtime interactive queries on program data
No full textAbstractPico ql is an open source c/c++ software whose scientific scope is real-time interactive analysis of in-memory data through sql queries. It exposes a relational view of a system’s or application’s data structures, which is queryable through sql. While the application or system is executing, users can input queries through a web-based interface or issue web service requests. Queries execute on the live data structures through the respective relational views. pico ql makes a good candidate for ad-hoc data analysis in applications and for diagnostics in systems settings. Applications of pico ql include the Linux kernel, the Valgrind instrumentation framework, a gis application, a virtual real-time observatory of stellar objects, and a source code analyser
Inhibition of microbial activity of activated sludge by ammonia in leachate
No full textAuthor name used in this publication: X. L. LiAccepted ManuscriptPublishedGreen (AAM
Recovery of ammonium-nitrogen from landfill leachate as a multi-nutrient fertilizer
No full textAuthor name used in this publication: X. Z. Li2002-2003 > Academic research: refereed > Publication in refereed journalAccepted ManuscriptPublishedGreen (AAM
Efficiency of biological treatment affected by high strength of ammonium-nitrogen in leachate and chemical precipitation of ammonium-nitrogen as pretreatment
No full textAuthor name used in this publication: X. Z. Li2000-2001 > Academic research: refereed > Publication in refereed journalAccepted ManuscriptPublishedGreen (AAM
Map precipitation from landfill leachate and seawater bittern waste
No full textAuthor name used in this publication: X. Z. Li2002-2003 > Academic research: refereed > Publication in refereed journalAccepted ManuscriptPublishedGreen (AAM
Quantitative strain analysis of misfit dislocations in a Ge/Si heterostructure interface by geometric phase analysis
No full textTo Extract Ontology Attribute Value Automatically Based on WWW
No full textAttribute value is among the most important information to describe Ontology. However, few researches have been done about attribute values extraction so fir. This paper proposes a method of extracting Ontology attribute values automatically based on WWW. Firstly, a method based on a seeds set is described about interaction between relevant sentences selection including attribute values and attribute values extraction, so that we can extract and expand the target attribute value set by the redundancy of WWW. Secondly, we construct the seeds set with an automatic method instead of by hand. Finally, we build hierarchical clusters of the candidate attribute values to gain more accurate and complete results. Experiments have been done to compute the precision and recall. Also automatically enriched Ontology information is used in webpage content extraction to show its usage.http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000266767300046&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701Computer Science, Theory & MethodsEngineering, Electrical & ElectronicEICPCI-S(ISTP)
Lignin bioconversion based on genome mining for ligninolytic genes in Erwinia billingiae QL-Z3
No full textAbstract Background Bioconversion of plant biomass into biofuels and bio-products produces large amounts of lignin. The aromatic biopolymers need to be degraded before being converted into value-added bio-products. Microbes can be environment-friendly and efficiently degrade lignin. Compared to fungi, bacteria have some advantages in lignin degradation, including broad tolerance to pH, temperature, and oxygen and the toolkit for genetic manipulation. Results Our previous study isolated a novel ligninolytic bacterial strain Erwinia billingiae QL-Z3. Under optimized conditions, its rate of lignin degradation was 25.24% at 1.5 g/L lignin as the sole carbon source. Whole genome sequencing revealed 4556 genes in the genome of QL-Z3. Among 4428 protein-coding genes are 139 CAZyme genes, including 54 glycoside hydrolase (GH) and 16 auxiliary activity (AA) genes. In addition, 74 genes encoding extracellular enzymes are potentially involved in lignin degradation. Real-time PCR quantification demonstrated that the expression of potential ligninolytic genes were significantly induced by lignin. 8 knock-out mutants and complementary strains were constructed. Disruption of the gene for ELAC_205 (laccase) as well as EDYP_48 (Dyp-type peroxidase), ESOD_1236 (superoxide dismutase), EDIO_858 (dioxygenase), EMON_3330 (monooxygenase), or EMCAT_3587 (manganese catalase) significantly reduced the lignin-degrading activity of QL-Z3 by 47–69%. Heterologously expressed and purified enzymes further confirmed their role in lignin degradation. Fourier transform infrared spectroscopy (FTIR) results indicated that the lignin structure was damaged, the benzene ring structure and groups of macromolecules were opened, and the chemical bond was broken under the action of six enzymes encoded by genes. The abundant enzymatic metabolic products by EDYP_48, ELAC_205 and ESOD_1236 were systematically analyzed via liquid chromatography–mass spectrometry (LC–MS) analysis, and then provide a speculative pathway for lignin biodegradation. Finally, The activities of ligninolytic enzymes from fermentation supernatant, namely, LiP, MnP and Lac were 367.50 U/L, 839.50 U/L, and 219.00 U/L by orthogonal optimization. Conclusions Our findings provide that QL-Z3 and its enzymes have the potential for industrial application and hold great promise for the bioconversion of lignin into bioproducts in lignin valorization
- …
