2,137 research outputs found
Supporting_Information – Supplemental material for A one-pot synthesis of benzimidazoles via aerobic oxidative condensation of benzyl alcohols with <i>o</i>-phenylenediamines catalyzed by [MIMPs]<sup>+</sup>Cl<sup>-</sup>/NaNO<sub>2</sub>/TEMPO
No full textSupplemental material, Supporting_Information for A one-pot synthesis of benzimidazoles via aerobic oxidative condensation of benzyl alcohols with o-phenylenediamines catalyzed by [MIMPs]+Cl-/NaNO2/TEMPO by Zhenzhen Geng, Hong-yu Zhang, Guohui Yin, Yuecheng Zhang and Jiquan Zhao in Journal of Chemical Research</p
The packaging of DNA in chromatin:
No full textThe mechanical properties of DNA play a key role in its biological processing, determining how the long, thin, double-helical molecule responds to the binding of proteins and functions in confined spaces within a cell. In eukaryotes, about 75-90% of genomic DNA exists in the form of nucleosomes, which are the fundamental units of DNA packaging in chromatin and the primary determinate of DNA accessibility. The structure of chromatin undergoes various changes that depend, at least in part, upon the requirements of gene expression and other functional environments. The dynamics of DNA packaging in chromatin is thus fundamental to numerous biological processes.
The flexibility of DNA is important in packaging DNA over lengths comparable to its persistence length during genetic processing and the sequence-dependent properties of DNA determine the positioning of nucleosomes in the genome and the sites of binding of enzymes and transcription factors. In addition, understanding the correlation between DNA flexibility and histone-DNA interactions inside the nucleosome is essential for unraveling currently unsolved mechanisms of gene regulation. Furthermore, although many experimental techniques have emerged to examine the overall structure of chromatin fibers, the internal arrangement of DNA and histones remains unclear. Thus an appropriate computational model able to incorporate experimental observations is key to interpretation of the folding and unfolding of chromatin.
The major goal of this thesis is to understand some of biophysical mechanisms involved in the packaging of DNA into chromatin using computational techniques at multi-scales: (i) to determine the sequence-dependent flexibility of DNA by developing DNA deformation analysis tools and databases; (ii) to design DNA spatial configurations using knowledge-based Monte-Carlo sampling; (iii) to map protein-DNA recognition inside nucleosomes in terms of realistic molecular treatments; and (iv) to interpret the internal structure of chromatin fibers and examine chromatin looping using novel modeling and simulation methods.Ph.D.Includes bibliographical referencesby Guohui Zhen
传染病智慧化症状监测的进展与挑战
No full textIntelligent syndromic surveillance is an important part of multi-point triggering and multi-channel surveillance system of intelligent early warning of infectious diseases in China, and an inevitable development process of traditional syndromic surveillance as the constant emergence of new technologies. Intelligent syndromic surveillance collects not only the medical data of patients seeking medical care in hospitals but also massive non-medical information. However, along with its rapid development, challenges in intelligent syndromic surveillance have emerged, such as information explosion, cost-effective balance, information sharing, data security and privacy. This paper summarizes the concept and development of intelligent syndromic surveillance to provide references for the method and technique development of intelligent early warning of infectious diseases and new thought for the prevention and control of infectious diseases in China and in the world
sj-docx-4-jet-10.1177_15266028211036479 – Supplemental material for Extended Stent Coverage Decreases Distal Aortic Segmental Enlargement After the Endovascular Repair of Acute Complicated Type B Aortic Dissection: A Multi-Center Retrospective Study of 814 Patients
No full textSupplemental material, sj-docx-4-jet-10.1177_15266028211036479 for Extended Stent Coverage Decreases Distal Aortic Segmental Enlargement After the Endovascular Repair of Acute Complicated Type B Aortic Dissection: A Multi-Center Retrospective Study of 814 Patients by Junjun Liu, Chaojun Yan, Lubin Li, Hai Feng, Shengmao Xie, Guohui Zhang, Wei Cheng, Mingjin Guo and MingYuan Liu in Journal of Endovascular Therapy</p
sj-docx-1-jet-10.1177_15266028211036479 – Supplemental material for Extended Stent Coverage Decreases Distal Aortic Segmental Enlargement After the Endovascular Repair of Acute Complicated Type B Aortic Dissection: A Multi-Center Retrospective Study of 814 Patients
No full textSupplemental material, sj-docx-1-jet-10.1177_15266028211036479 for Extended Stent Coverage Decreases Distal Aortic Segmental Enlargement After the Endovascular Repair of Acute Complicated Type B Aortic Dissection: A Multi-Center Retrospective Study of 814 Patients by Junjun Liu, Chaojun Yan, Lubin Li, Hai Feng, Shengmao Xie, Guohui Zhang, Wei Cheng, Mingjin Guo and MingYuan Liu in Journal of Endovascular Therapy</p
sj-docx-2-jet-10.1177_15266028211036479 – Supplemental material for Extended Stent Coverage Decreases Distal Aortic Segmental Enlargement After the Endovascular Repair of Acute Complicated Type B Aortic Dissection: A Multi-Center Retrospective Study of 814 Patients
No full textSupplemental material, sj-docx-2-jet-10.1177_15266028211036479 for Extended Stent Coverage Decreases Distal Aortic Segmental Enlargement After the Endovascular Repair of Acute Complicated Type B Aortic Dissection: A Multi-Center Retrospective Study of 814 Patients by Junjun Liu, Chaojun Yan, Lubin Li, Hai Feng, Shengmao Xie, Guohui Zhang, Wei Cheng, Mingjin Guo and MingYuan Liu in Journal of Endovascular Therapy</p
Data for: Change of the abundances of Fe, Ni and Zr isotopes due to neutron radiative capture reactions in heavy water reactors
No full textThis is the data for in the pape
sj-png-7-jet-10.1177_15266028211036479 – Supplemental material for Extended Stent Coverage Decreases Distal Aortic Segmental Enlargement After the Endovascular Repair of Acute Complicated Type B Aortic Dissection: A Multi-Center Retrospective Study of 814 Patients
No full textSupplemental material, sj-png-7-jet-10.1177_15266028211036479 for Extended Stent Coverage Decreases Distal Aortic Segmental Enlargement After the Endovascular Repair of Acute Complicated Type B Aortic Dissection: A Multi-Center Retrospective Study of 814 Patients by Junjun Liu, Chaojun Yan, Lubin Li, Hai Feng, Shengmao Xie, Guohui Zhang, Wei Cheng, Mingjin Guo and MingYuan Liu in Journal of Endovascular Therapy</p
Molecular mechanism of FAT signaling pathway coordinating growth and patterning in drosphila melanogaster
No full textFrom an RNAi lines screen, our lab found the Drosophila Zyxin family gene, Zyx102 (Zyx) as a negative regulator of Fat-Hippo signaling. Epistasis analysis put Zyx between Dachs and Warts (Wts) in the Fat-branch, and Zyx-RNAi suppresses the reduction of Wts level by fat RNAi. Zyx partially co-localizes with Dachs, and its localization was not altered by manipulating other Fat-branch component. Zyx protein binds to Dachs, and Dachs stimulates binding of Zyx to Wts, suggesting a molecular mechanism for how Fat signaling regulates Wts through Dachs. Our results identified a new component of Fat signaling, and investigated possible molecular mechanism for signal transduction from Fat to Wts. Among the vast amount of genes involved in organ morphogenesis, Fat gene stands out as it transduces both growth cue and planar cell polarity (PCP). But the molecular mechanism underlying is unknown. A structure-function approach is recruited to figure out the motifs in Fat that are required for signal transduction mechanism. By a fat genomic Bac Clone, which is expressed under endogenous condition, activities that could be missed by over-expression can be revealed. We find out the PCP activity is evolutionarily conserved from Drosophila to mammals. And the Hippo and PCP activity can be largely separated at the level of the Fat receptor. A specific motif was identified to mainly impair Fat-Hippo activity and reduce Fat phosphorylation. Manipulating the potential phosphorylation sites in this motif gives similar phenotypes, implicating phosphorylation as an essential factor in Fat-Hippo signal transduction. This motif overlaps with the previously identified Dco binding site, but retains normal binding affinity. Another conserved four amino acid motif is crucial for Fat-PCP, while the Fat-Hippo activity is almost normal. And this motif contributes differently to multiple PCP assays, suggesting there are different kinds of PCP in Drosophila. Different Fat activity leads to distinctive Dachs localization pattern, and directly manipulating Dachs localization could phenocopy loss of Fat activity. Thus Dachs localization influences both Hippo and PCP phenotypes. Our results identify a conserved mechanism of Fat signaling, establish functional significances for Fat ICD motif, phosphorylation sites, and Dachs membrane localization to downstream pathways.Ph. D.Includes bibliographical referencesIncludes vitaby Guohui Pa
Data for: Change of the abundances of Fe, Ni and Zr isotopes due to neutron radiative capture reactions in heavy water reactors
No full textThis is the data for in the pape
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